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Multivariate ratio analysis and DNA markers reveal a new Australian species and three synonymies in eucalypt-gall-associated Megastigmus (Hymenoptera: Megastigmidae)

Published online by Cambridge University Press:  27 May 2020

Ngoc Hoan Le Open the ORCID record for Ngoc Hoan Le [Opens in a new window] ,
Helen F. Nahrung Open the ORCID record for Helen F. Nahrung [Opens in a new window] ,
Jess A. T. Morgan  and
Simon A. Lawson Open the ORCID record for Simon A. Lawson [Opens in a new window]
Ngoc Hoan Le*
Affiliation:
Forest Industries Research Centre, University of the Sunshine Coast, Sippy Downs, QLD4556, Australia
Helen F. Nahrung
Affiliation:
Forest Industries Research Centre, University of the Sunshine Coast, Sippy Downs, QLD4556, Australia
Jess A. T. Morgan
Affiliation:
Department of Agriculture and Fisheries, EcoSciences Precinct, PO Box 267, BrisbaneQueensland4001, Australia
Simon A. Lawson
Affiliation:
Forest Industries Research Centre, University of the Sunshine Coast, Sippy Downs, QLD4556, Australia
*
Author for correspondence: Ngoc Hoan Le, Email: [email protected]
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Abstract

The genus Megastigmus Dalman, 1820 (Hymenoptera: Megastigmidae) contains potential biocontrol agents of the invasive eucalypt galling chalcid Leptocybe spp. (Hymenoptera: Eulophidae), with several species reported in various parts of the world. Species discrimination is challenging due to intraspecific morphological variation, difficulty in measuring sizes of body parts, and the lack of information regarding the global distribution of parasitic Megastigmus. We used two species commonly associated with Leptocybe in its native range to review taxonomic methods and determine the most reliable morphological characters in species delimitation. We examined size variation of body characters, and conducted species discrimination using multivariate ratio analysis, mitochondrial Cytochrome c oxidase subunit 1 (COI) and nuclear 28S rDNA (28S) sequences. Morphological traits were effective in species delimitation yet revealed high variation in several characters employed in current keys. Knowledge generated on morphology and DNA justified the description of a new species, M. manonae, sp. n., the first record of M. pretorianensis in Australia, and revised diagnostic characters for M. zvimendeli. Based on these diagnostic characters and molecular data, we synonymize three species (M. judikingae, syn. n., from Australia, M. sichuanensis, syn. n., from China and M. icipeensis, syn. n., from Kenya) with M. zvimendeli. Our findings highlight the importance of molecular markers in assisting taxonomic decision-making and the need for coordinated work in identifying Megastigmus associated with Leptocybe spp.

Keywords

LeptocybeMegastigmus manonaeMegastigmus zvimendelimorphologyparasitoidsspecies discrimination
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 110 , Issue 6 , December 2020 , pp. 709 - 724
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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