Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-26T16:02:25.279Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular diagnostics of economically important Ceratitis fruit fly species (Diptera: Tephritidae) in Africa using PCR and RFLP analyses

Published online by Cambridge University Press:  09 March 2007

N.B. Barr ,
R.S. Copeland ,
M. De Meyer ,
D. Masiga ,
H.G. Kibogo ,
M.K. Billah ,
E. Osir ,
R.A. Wharton  and
B.A. McPheron
N.B. Barr*
Affiliation:
Department of Entomology, Pennsylvania State University, University Park, PA 16802, USA Center for Plant Health Science and Technology, Pest Detection Diagnostics and Management Laboratory, USDA-APHIS, Moore Air Base, Edinburg, TX 78541, USA
R.S. Copeland
Affiliation:
Department of Entomology, Texas A&M University, College Station, TX 77843, USA International Centre of Insect Physiology and Ecology, PO Box 30772, Nairobi, Kenya
M. De Meyer
Affiliation:
Royal Museum for Central Africa, Entomology Section, Belgium
D. Masiga
Affiliation:
International Centre of Insect Physiology and Ecology, PO Box 30772, Nairobi, Kenya Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, Kenya
H.G. Kibogo
Affiliation:
International Centre of Insect Physiology and Ecology, PO Box 30772, Nairobi, Kenya
M.K. Billah
Affiliation:
International Centre of Insect Physiology and Ecology, PO Box 30772, Nairobi, Kenya
E. Osir
Affiliation:
International Centre of Insect Physiology and Ecology, PO Box 30772, Nairobi, Kenya
R.A. Wharton
Affiliation:
Department of Entomology, Texas A&M University, College Station, TX 77843, USA
B.A. McPheron
Affiliation:
Department of Entomology, Pennsylvania State University, University Park, PA 16802, USA
*
*Fax: (956) 580 7300 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The predominantly Afrotropical fruit fly genus Ceratitis contains many species of agricultural importance. Consequently, quarantine of Ceratitis species is a major concern for governmental regulatory agencies. Although diagnostic keys exist for identification of all described Ceratitis species, these tools are based on adult characters. Flies intercepted at ports of entry are usually immatures, and Ceratitis species cannot be diagnosed based on larval morphology. To facilitate identification of Ceratitis pests at ports of entry, this study explores the utility of DNA-based diagnostic tools for a select group of Ceratitis species and related tephritids, some of which infest agriculturally important crops in Africa. The application of the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method to analyse three mitochondrial genes (12S ribosomal RNA, 16S ribosomal RNA, and NADH-dehydrogenase subunit 6) is sufficient to diagnose 25 species and two species clusters. PCR analysis of the internal transcribed spacer region 1 (ITS-1) is able to distinguish three of the five species left unresolved by mitochondrial DNA analysis.

Keywords

mitochondrial DNAITSCeratitisquarantine
Type
Research Article
Information
Bulletin of Entomological Research , Volume 96 , Issue 5 , October 2006 , pp. 505 - 521
Copyright
Copyright © Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Armstrong, K.F. & Ball, S.L. (2005) DNA barcodes for biosecurityΧ invasive species identification. Philosophical Transactions of the Royal Society B 360, 1813–1823.CrossRefGoogle ScholarPubMed
Armstrong, K.F., Cameron, C.M. & Frampton, E.R. (1997) Fruit fly (DipteraΧ Tephritidae) species identificationΧ a rapid molecular diagnostic technique for quarantine application. Bulletin of Entomological Research 87, 111–118.CrossRefGoogle Scholar
Baliraine, F.N., Bonizzoni, M., Osir, E.O., Lux, S.A., Mulaa, F.J., Zheng, L., Gomulski, L.M., Gasperi, G. & Malacrida, A.R. (2003) Comparitive analysis of microsatellite loci in four fruit fly species of the genus Ceratitis (DipteraΧ Tephritidae). Bulletin of Entomological Research 93, 1–10.CrossRefGoogle Scholar
Baliraine, F.N., Bonizzoni, M., Guglielmino, C.R, Osir, E.O., Lux, S.A., Mulaa, F.J., Gomulski, L.M., Zheng, L., Quilici, S., Gasperi, G. & Malacrida, A.R. (2004) Population genetics of the potentially invasive African fruit fly species, Ceratitis rosa and Ceratitis fasciventris (DipteraΧ Tephritidae). Molecular Ecology 13, 683–695.CrossRefGoogle ScholarPubMed
Barr, N.B. (2004) Molecular diagnostics and systematics of the genus Ceratitis (Tephritidae). PhD thesis, The Pennsylvania State University, Pennsylvania.Google Scholar
Barr, N.B. & McPheron, B.A. (2006) Molecular phylogenetics of the genus Ceratitis (DipteraΧTephritidae). Molecular Phylogenetics and Evolution 38, 216–230.CrossRefGoogle Scholar
Carey, J.R. (1984) Host-specific demographic studies of the Mediterranean fruit fly, Ceratitis capitata. Ecological Entomology 9, 261–270.CrossRefGoogle Scholar
Copeland, R.S., Wharton, R.A., Luke, Q. & De Meyer, M. (2002) Indigenous hosts of Ceratitis capitata (DipteraΧTephritidae) in Kenya. Annals of the Entomological Society of America 95, 672–694.CrossRefGoogle Scholar
De Meyer, M. (1996) Revision of the subgenus Ceratitis (Pardalaspis) Bezzi, 1918 (DipteraΧTephritidaeΧ Ceratitini). Systematic Entomology 21, 15–26.CrossRefGoogle Scholar
De Meyer, M. (1998) Revision of the subgenus Ceratitis (Ceratalaspis) Hancock (DipteraΧ Tephritidae). Bulletin of Entomological Research 88, 257–290.CrossRefGoogle Scholar
De Meyer, M. (2000) Systematic revision of the subgenus Ceratitis MacLeay s. s. (DipteraΧ Tephritidae). Zoological Journal of the Linnean Society 128, 439–467.CrossRefGoogle Scholar
De Meyer, M. (2001a) Distribution patterns and host-plant relationships within the genus Ceratitis MacLeay (DipteraΧTephritidae) in Africa. Cimbebasia 17, 219–228.Google Scholar
De Meyer, M. (2001b) On the identity of the Natal fruit fly Ceratitis rosa Karsch (Diptera, Tephritidae). Entomologie 71, 55–62.Google Scholar
De Meyer, M. & Copeland, R.S. (2005) Description of new Ceratitis MacLeay (DipteraΧTephritidae) species from Africa. Journal of Natural History 39, 1283–1297.CrossRefGoogle Scholar
De Meyer, M. & Freidberg, A. (in press) Revision of the subgenus Ceratitis (Pterandrus) Bezzi (DipteraΧTephritidae). Proceedings of the 1st Tephridologists MeetingIsrael.Google Scholar
De Meyer, M., Copeland, R.S., Lux, S.A., Mansell, M., Quilici, S., Wharton, R., White, I.M. & Zenz, N.J. (2002) Annotated check list of host plants for afrotropical fruit flies (Diptera: Tephritidae) of the genus Ceratitis. Documentations Zoologiques, Musee Royal de l'Afrique Centrale 27, 1–91.Google Scholar
De Meyer, M., Copeland, R.S., Wharton, R.A. and McPheron, B.A. (2004) On the geographical origin of the medfly, Ceratitis capitata (Wiedemann) (DipteraΧTephritidae). Proceedings of the 6th International Symposium on Fruit Flies of Economic Importance, Stellenbosch, South Africa, pp. 45–53.Google Scholar
Douglas, L.J. & Haymer, D.S. (2001) Ribosomal ITS1 polymorphism in Ceratitis capitata and Ceratitis rosa (DipteraΧTephritidae). Annals of the Entomological Society of America 94, 726–731.CrossRefGoogle Scholar
Duyck, P.F. & Quilici, S. (2002) Survival and development of different life stages of three Ceratitis spp. (DipteraΧTephritidae) reared at five constant temperatures. Bulletin of Entomological Research 92, 461–469.CrossRefGoogle ScholarPubMed
Duyck, P.F., David, P. & Quilici, S. (2004) A review of relationships between interspecific competition and invasions in fruit flies (DipteraΧTephritidae). Ecological Entomology 29, 511–520.CrossRefGoogle Scholar
Freidberg, A. (1991) A new species of Ceratitis (Ceratitis) (DipteraΧTephritidae), key to species of subgenera Ceratitis and Pterandrus, and record of Pterandrus fossil. Bishop Museum Occasional Papers 31, 166–173.Google Scholar
Gasparich, G.E., Sheppard, W.S., Han, H.-Y., McPheron, B.A. & Steck, G.J. (1995) Analysis of mitochondrial DNA and development of PCR-based diagnostic markers for Mediterranean fruit fly (Ceratitis capitata) populations. Insect Molecular Biology 4, 61–67.CrossRefGoogle ScholarPubMed
Gasparich, G.E., Silva, J.G., Han, H.-Y., McPheron, B.A., Steck, G.J. & Sheppard, W.S. (1997) Population genetic structure of the Mediterranean fruit fly (DipteraΧTephritidae) and implications for worldwide colonization patterns. Annals of the Entomological Society of America 90, 790–797.CrossRefGoogle Scholar
Han, H.-Y. & McPheron, B.A. (1997) Molecular phylogenetic study of Tephritidae (InsectaΧDiptera) using partial sequences of the mitochondrial 16S ribosomal DNA. Molecular Phylogenetics and Evolution 7, 17–32.CrossRefGoogle ScholarPubMed
Han, H.-Y. & McPheron, B.A. (2000) Nucleotide sequence data as a tool to test phylogenetic relationships among higher groups of TephritidaeΧ a case study using mitochondrial ribosomal DNA. pp.115–134in Aluja, M. & Norrbom, A.L. (Eds) Fruit flies (Tephritidae)Χ phylogeny and evolution of behavior. Boca Raton, Florida, CRC Press.Google Scholar
Kakouli-Durante, T., Casey, D.G. & Burnell, A.M. (2001) Development of a diagnostic DNA probe for the fruit flies Ceratitis capitata and Ceratitis rosa (DipteraΧTephritidae) using amplified fragment-length polymorphism. Journal of Economic Entomology 94, 989–997.CrossRefGoogle Scholar
Kibogo, H.G. (2005). Identification of fruit fly species in the genera Ceratitis and Trirhithrum (DipteraΧTephritidae). MSc thesis, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.Google Scholar
Liquido, N.J., Barr, P.G. & Cunningham, R.T. (1998) MEDHOST, an encyclopedic bibliography of the host plants of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), version 1. Diptera dissemination disk 1 in Thompson, F.C. (Ed.) Fruit fly expert identification system and systematic information database.Google Scholar
Malacrida, A.R., Marinoni, F., Torti, C., Gomulski, L.M., Sebastiani, F., Bonvicini, C., Gasperi, G. & Gugliemino, C.R. (1998) Genetic aspects of the worldwide colonization process of Ceratitis capitata. Journal of Heredity 89, 501–507.CrossRefGoogle ScholarPubMed
Muraji, M. & Nakahara, S. (2002) Discrimination among pest species of Bactrocera (DipteraΧTephritidae) based on PCR–RFLP of the mitochondrial DNA. Applied Entomology and Zoology 37, 437–446.CrossRefGoogle Scholar
Naelole, C.K.M. & Haymer, D.S. (2003) Use of oligonucleotide arrays for molecular taxonomic studies of closely related species in the oriental fruit fly (Bactrocera dorsalis) complex. Molecular Ecology 3, 662–665.CrossRefGoogle Scholar
Ochando, M.D., Reyes, A., Callejas, C., Segura, D. & Fernandez, P. (2003) Molecular genetic methodologies applied to the study of fly pests. Trends in Entomology 3, 73–85.Google Scholar
Salazar, M., Theoduloz, C., Vega, A., Poblete, F., Gonzalez, E., Badilla, R. & Meza-Basso, L. (2002) PCR–RFLP identification of endemic Chilean species of Rhagoletis (DipteraΧTephritidae) attacking Solanaceae. Bulletin of Entomological Research 92, 337–341.CrossRefGoogle ScholarPubMed
Saunders, M., Coulson, R.N. & Folse, L.J. (1993) Natural resource management and agriculture, applications and artificial intelligence. pp.149–162Kent, A., Williams, J.G. (Eds)Encyclopedia of microcomputers vol. 12, multistrategy learning to operations research, microcomputers applications. New York, Marcel Dekker, Inc.Google Scholar
Saunders, M.C. and Miller, B.J. (1997). A graphical tool for knowledge engineers designing natural resource management softwareΧNetWeaver. 1997 ACSM/ASPRS Annual Convention and Exposition Technical Papers, Seattle, Washington Χ Resource Technology, Vol. 4, 380–389.Google Scholar
Sheppard, W.S., Steck, G.J. and McPheron, B.A. (1992) Geographic populations of the medfly may be differentiated by mitochondrial DNA variation. Experientia 48, 1010–1013.CrossRefGoogle Scholar
Silva, J.G., Meixner, M.D., McPheron, B.A., Steck, G.J. & Sheppard, W.S. (2003) Recent Mediterranean fruit fly (DipteraΧTephritidae) infestations in Florida – a genetic perspective. Journal of Economic Entomology 96, 1711–1718.CrossRefGoogle Scholar
Smith, P.T., McPheron, B.A. & Kambhampati, S. (2002) Phylogenetic analysis of mitochondrial DNA supports the monophyly of Dacini fruit flies (DipteraΧTephritidae). Annals of the Entomological Society of America 95, 658–664.CrossRefGoogle Scholar
Spanos, L., Koutroumbas, G., Kotsyfakis, M. & Louis, C. (2000) The mitochondrial genome of the Mediterranean fruit fly, Ceratitis capitata. Insect Molecular Biology 9, 139–144.CrossRefGoogle ScholarPubMed
Steck, G.J., Carroll, L.E., Celedonio, H. & Guillen, J.C. (1990) Methods for identification of Anastrepha larvae (DipteraΧTephritidae), and key to 13 species. Proceedings of the Entomological Society of Washington 92, 333–346.Google Scholar
Vera, M.T., Rodriguez, R., Segura, D.F., Cladera, J.L. & Sutherst, R.W. (2002) Potential geographical distribution of the Mediterranean fruit fly, Ceratitis capitata (DipteraΧTephritidae), with emphasis on Argentina and Australia. Environmental Entomology 31, 1009–1022.CrossRefGoogle Scholar
White, I.M. & Elson-Harris, M.M. (1992) Fruit flies of economic significanceΧ their identification and bionomics. 600 pp. Wallingford, Oxon, CAB International.CrossRefGoogle Scholar
Yuval, B. & Hendrichs, J. (2000) Behavior of flies in the genus Ceratitis (DacinaeΧCeratitidini). pp. 429–457in Aluja, M. & Norrbom, A.L. (Eds) Fruit flies (Tephritidae): phylogeny and evolution of behavior. Boca Raton, Florida, CRC Press.Google Scholar