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Investigation of insecticide-resistance status of Cydia pomonella in Chinese populations

Published online by Cambridge University Press:  17 March 2015

X.-Q. Yang  and
Y.-L. Zhang
X.-Q. Yang
Affiliation:
Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A & F University, Yangling712100, Shaanxi, China Key Laboratory of Economical and Applied Entomology of Liaoning province, College of Plant Protection, Shenyang Agriculture University, Shenyang110866, Liaoning, China
Y.-L. Zhang*
Affiliation:
Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A & F University, Yangling712100, Shaanxi, China
*
*Author for correspondence Phone: +86-29-8709-2190 Fax: +86-29-8709-2190 E-mail: [email protected]
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Abstract

The codling moth Cydia pomonella (L.) is an economically important fruit pest and it has been directly targeted by insecticides worldwide. Serious resistance to insecticides has been reported in many countries. As one of the most serious invasive pest, the codling moth has populated several areas in China. However, resistance to insecticides has not been reported in China. We investigated the insecticide-resistance status of four field populations from Northwestern China by applying bioassays, enzyme activities, and mutation detections. Diagnostic concentrations of lambda-cyhalothrin, chlorpyrifos-ethyl, carbaryl, and imidacloprid were determined and used in bioassays. Field populations were less susceptible to chlorpyrifos-ethyl and carbaryl than laboratory strain. Insensitive populations displayed an elevated glutathione S-transferases (GSTs) activity. Reduced carboxylesterase (CarE) activity was observed in some insecticide insensitive populations and reduced acetylcholinesterase activity was observed only in the Wuw population. The cytochrome P450 polysubstrate monooxygenases activities in four field populations were not found to be different from susceptible strains. Neither the known-resistance mutation F399V in the acetylcholinesterase (AChE) gene, ace1, nor mutations in CarE gene CpCE-1 were found in adult individuals from our field populations. Native-PAGE revealed that various CarE isozymes and AChE insensitivity were occurring among Chinese populations. Our results indicate that codling moth populations from Northwestern China were insensitivity to chlorpyrifos-ethyl and carbaryl. Increased GST activity was responsible for insecticides insensitivity. Decreased CarE activity, as well as the presence of CarE and AChE polymorphisms might also be involved in insecticides insensitivity. New management strategies for managing this pest are discussed.

Keywords

Cydia pomonellainsecticides resistancedetoxifying enzymesmanagementinvasions
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 105 , Issue 3 , June 2015 , pp. 316 - 325
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Copyright
Copyright © Cambridge University Press 2015 

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