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Exploring plant responses to aphid feeding using a full Arabidopsis microarray reveals a small number of genes with significantly altered expression

Published online by Cambridge University Press:  04 October 2007

C. Couldridge ,
H.J. Newbury ,
B. Ford-Lloyd ,
J. Bale  and
J. Pritchard
C. Couldridge
Affiliation:
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
H.J. Newbury
Affiliation:
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
B. Ford-Lloyd
Affiliation:
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
J. Bale
Affiliation:
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
J. Pritchard*
Affiliation:
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
*
*Author for correspondence Fax: 0121 414 5925 E-mail: [email protected]
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Abstract

The aim of this study was to determine which Arabidopsis thaliana (L.) genes had significantly altered expression following 2–36 h of infestation by the aphid Myzus persicae (Sulzer). Six biological replicates were performed for both control and treatment at each time point, allowing rigorous statistical analysis of any changes. Only two genes showed altered expression after 2 h (one up- and one down-regulated) while two were down-regulated and twenty three were up-regulated at 36 h. The transcript annotation allowed classification of the significantly altered genes into a number of classes, including those involved in cell wall modification, carbon metabolism and signalling. Additionally, a number of genes were implicated in oxidative stress and defence against other pathogens. Five genes could not currently be assigned any function. The changes in gene expression are discussed in relation to current models of plant-insect interactions.

Keywords

aphid feedingMyzus persicaephloemsieve elementmicroarray Arabidopsis thalianafunctional genomicsdefence
Type
Research Article
Information
Bulletin of Entomological Research , Volume 97 , Issue 5 , October 2007 , pp. 523 - 532
Copyright
Copyright © Cambridge University Press 2007

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