Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-20T07:31:29.990Z Has data issue: false hasContentIssue false
  • English
  • Français

Enantiomeric selectivity in behavioural and electrophysiological responses of Aedes aegypti and Culex quinquefasciatus mosquitoes

Published online by Cambridge University Press:  18 May 2011

J.I. Cook ,
S. Majeed ,
R. Ignell ,
J.A. Pickett ,
M.A. Birkett  and
J.G. Logan
J.I. Cook
Affiliation:
Centre for Sustainable Pest and Disease Management, Chemical Ecology Group, Biological Chemistry Department, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK
S. Majeed
Affiliation:
Division of Chemical Ecology, Department of Plant Protection Biology, PO Box 102, Sundsvägen 14, SLU 230 53, Alnarp, Sweden
R. Ignell
Affiliation:
Division of Chemical Ecology, Department of Plant Protection Biology, PO Box 102, Sundsvägen 14, SLU 230 53, Alnarp, Sweden
J.A. Pickett
Affiliation:
Centre for Sustainable Pest and Disease Management, Chemical Ecology Group, Biological Chemistry Department, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK
M.A. Birkett
Affiliation:
Centre for Sustainable Pest and Disease Management, Chemical Ecology Group, Biological Chemistry Department, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK
J.G. Logan*
Affiliation:
Department of Disease Control, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
*
*Author for correspondence Fax: +44 (0)207 927 2918 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

1-Octen-3-ol is a kairomone for many haematophagous insects including mosquitoes. Numerous studies have examined the effects of racemic 1-octen-3-ol; however, few studies have investigated the role of individual enantiomers in relation to mosquito attraction. In the present study, we investigated the behavioural and electrophysiological responses of two mosquito species, Aedes aegypti and Culex quinquefasciatus, to individual enantiomers and mixtures of 1-octen-3-ol, employing a laboratory Y-tube olfactometer and single sensillum recordings. The olfactory receptor neurons of both Ae. aegypti and Cx. quinquefasciatus had a significantly higher response to the (R)-1-octen-3-ol enantiomer compared to the (S)-1-octen-3-ol enantiomer at 10−9 g μl−1 to 10−6 g μl−1. Behaviourally, Ae. aegypti was more responsive to the (R)-1-octen-3-ol enantiomer, showing an increase in flight activity and relative attraction compared to Cx. quinquefasciatus. The (R)-1-octen-3-ol enantiomer caused an increase in activation for Cx. quinquefasciatus. However, the most notable effect was from an (R:S)-1-octen-3-ol mixture (84:16) that caused significantly more mosquitoes to sustain their flight and reach the capture chambers (demonstrated by a reduced non-sustained flight activity), suggesting that it may have a behaviourally excitatory effect. For Cx. quinquefasciatus, a reduced relative attraction response was also observed for all treatments containing the (R)-1-octen-3-ol enantiomer, either on its own or as part of a mixture, but not with the (S)-1-octen-3-ol enantiomer. This is the first time enantiomeric selectivity has been shown for Ae. aegypti using electrophysiology in vivo. The implications of these results for exploitation in mosquito traps are discussed.

Keywords

mosquito1-octen-3-olenantiomerelectrophysiologybehaviour
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 101 , Issue 5 , October 2011 , pp. 541 - 550
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Becker, N., Zgomba, M., Petric, D. & Ludwig, M. (1995) Comparison of carbon dioxide, octenol and a host-odour as mosquito attractants in the Upper Rhine Valley, Germany. Medical and Veterinary Entomology 9, 377–380.CrossRefGoogle Scholar
Bohbot, J.D. & Dickens, J.C. (2009) Characterization of an enantioselective odourant receptor in the yellow fever mosquito Aedes aegypti. PloS One 4, e7032.Google Scholar
Burkett, D.A., Lee, W.J., Lee, K.W., Kim, H.C., Lee, H.I., Lee, J.S., Shin, E.H., Wirtz, R.A., Cho, H.W., Claborn, D.M., Coleman, R.E. & Klein, T.A. (2001) Light, carbon dioxide, and octenol-baited mosquito trap and host seeking activity evaluations for mosquitoes in a malarious area of the Republic of Korea. Journal of the American Mosquito Control Association 17, 196–205.Google Scholar
Bursell, E. (1984) Effects of host odour on the behaviour of tsetse. Insect Science and its Application 5, 345–349.Google Scholar
Canyon, D.V. & Hii, J.L.K. (1997) Efficacy of carbon dioxide, 1-octen-3-ol, and lactic acid in modified Fay-Prince traps as compared to man-landing catch of Aedes aegypti. Journal of the American Mosquito Control Association 13, 66–70.Google ScholarPubMed
Eiras, A.E. & Jepson, P.C. (1994) Responses of female Aedes aegypti (Diptera: Culicidae) to host odours and convection currents using an olfactometer bioassay. Bulletin of Entomological Research 84, 207–211.CrossRefGoogle Scholar
Gibson, G. & Torr, S.J. (1999) Visual and olfactory responses of haematophagous Diptera to host stimuli. Medical and Veterinary Entomology 13, 2–23.Google Scholar
Hall, D.R., Beevor, P.S., Cork, A., Nesbitt, B.F. & Vale, G.A. (1984) 1-Octen-3-ol a potent olfactory stimulant and attractant for tsetse isolated from cattle odours. Insect Science and its Application 5, 335–339.Google Scholar
Hill, S.R., Hansson, B.S. & Ignell, R. (2009) Characterization of antennal trichoid sensilla from female southern house mosquito, Culex quinquefasciatus Say. Chemical Senses 34(3), 231–252.Google Scholar
Kemme, J.A., Van Essen, P.H.A., Ritchie, S.A. & Kay, B.H. (1993) Response of mosquitoes to carbon dioxide and 1-octen-3-ol in southeast Queensland, Australia. Journal of the American Mosquito Control Association 9, 431–435.Google ScholarPubMed
Khan, A.A., Maibach, H.I., Strauss, W.G. & Fenley, W.R. (1966) Quantitation of effect of several stimuli on the approach of Aedes aegypti. Journal of Economic Entomology 59, 690–694.CrossRefGoogle ScholarPubMed
Kline, D.L. & Lemire, G.F. (1995) Field evaluation of heat as an added attractant to traps baited with carbon dioxide and octenol for Aedes taeniohynchus. Journal of the American Mosquito Control Association 11, 454–456.Google Scholar
Kline, D.L., Allan, S.A., Bernier, U.R. & Welch, C.H. (2007) Evaluation of the enantiomers of 1-octen-3-ol and 1-octyn-3-ol as attractants for mosquitoes associated with a freshwater swamp in Florida, U.S.A. Medical and Veterinary Entomology 21, 323–331.Google Scholar
Knudsen, J.T., Tollsten, L. & Bergstrom, G. (1993) Floral scents – A checklist of volatile compounds isolated by head-space techniques. Phytochemistry 33, 253–280.Google Scholar
Logan, J.G., Birkett, M.A., Clark, S.J., Powers, S., Seal, N.J., Wadhams, L.J., Mordue, A.J. & Pickett, J.A. (2008) Identification of human-derived volatile chemicals that interfere with attraction of Aedes aegypti mosquitoes. Journal of Chemical Ecology 34, 308–322.Google Scholar
Lu, T., Qiu, Y.T., Wang, G., Kwon, J.Y., Rutzler, M., Kwon, H.W., Pitts, R.J., Van Loon, J.J.A., Takken, W., Carson, J.R. & Zwiebel, L.J. (2007) Odor coding in the maxillay palp of the malaria vector mosquito Anopheles gambiae. Current Biology 17, 1533–1544.Google Scholar
Mboera, L.E.G., Takken, W. & Sambu, E.Z. (2000) The response of Culex quinquefasciatus (Diptera: Culicidae) to traps baited with carbon dioxide, 1-octen-3-ol, acetone, butyric acid and human foot odour in Tanzania. Bulletin of Entomological Research 90, 155–159.Google Scholar
McMahon, C., Guerin, P.M. & Syed, Z. (2001) 1-Octen-3-ol isolated from bont ticks attracts Amblyomma variegatum. Journal of Chemical Ecology 27, 471–486.CrossRefGoogle ScholarPubMed
Miller, R.J., Wing, J., Cope, S., Davey, R.B. & Kline, D.L. (2005) Comparison of carbon dioxide- and octenol-baited enchepalitis virus surveillance mosquito traps at the Shoalwater Bay Training area, Queensland, Australia. Journal of the American Mosquito Control Association 21, 497–500.CrossRefGoogle ScholarPubMed
Pates, H.V., Takken, W. & Curtis, C.F. (2005) Laboratory studies on the olfactory behaviour of Anopheles quadriannulatus. Entomologia Experimentalis et Applicata 114, 153–159.CrossRefGoogle Scholar
Pierce, A.M., Pierce, H.D. Jr, Borden, J.H. & Oehlschlager, A.C. (1989) Production dynamics of cucujolide pheromones and identification of 1-octen-3-ol as a new aggregation pheromone for Oryzaephilus surinamensis and O. mercator (Coleoptera: Cucujidae). Environmental Entomology 18, 747–755.CrossRefGoogle Scholar
Rueda, L.M., Harrison, B.A., Brown, J.S., Whitt, P.B., Harrison, R.L. & Gardner, R.C. (2001) Evaluation of 1-octen-3-ol, carbon dioxide, and light as attractants for mosquitoes associated with two distinct habitats in North Carolina. Journal of the American Mosquito Control Association 17, 61–66.Google ScholarPubMed
Russell, R.C. (2004) The relative attractiveness of carbon dioxide and octenol in CDC- and EVS-type light traps for sampling the mosquitoes Aedes aegypti (L.), Aedes polynesiensis Marks, and Culex quinquefasciatus Say in Moorea, French Polynesia. Journal of Vector Ecology 29, 309–314.Google Scholar
Shone, S.M., Ferrao, P.N., Lesser, C.R., Glass, G.E. & Norris, D.E. (2003) Evaluation of carbon dioxide- and 1-octen-3-ol-baited Centers for Disease Control Fay-Prince traps to collect Aedes albopictus. Journal of the American Mosquito Control Association 19, 445–447.Google Scholar
Syed, Z. & Leal, W.S. (2007) Maxillary palps are broad spectrum odourant detectors in Culex quinquefasciatus. Chemical Senses 32, 727–738.Google Scholar
Takken, W. (1991) The role of olfaction in host-seeking of mosquitoes: a review. Insect Science and its Application 12, 287–295.Google Scholar
Takken, W. & Kline, D.L. (1989) Carbon dioxide and 1-octen-3-ol as mosquito attractants. Journal of the American Mosquito Control Association 5, 311–316.Google ScholarPubMed
Vale, G.A. & Hall, D.R. (1985a) The role of 1-octen-3-ol, acetone and carbon dioxide in the attraction of tsetse flies, Glossina spp. (Diptera: Glossinidae), to ox odour. Bulletin of Entomological Research 75, 209–217.CrossRefGoogle Scholar
Vale, G.A. & Hall, D.R. (1985b) The use of 1-octen-3-ol, acetone and carbon dioxide to improve baits for tsetse flies, Glossina spp. (Diptera: Glossinidae). Bulletin of Entomological Research 75, 219–231.Google Scholar
Van Essen, P.H.A., Kemme, J.A., Ritchie, S.A. & Kay, B.H. (1994) Differential responses of Aedes and Culex mosquitoes to octenol or light in combination with carbon dioxide in Queensland, Australia. Medical and Veterinary Entomology 8, 63–67.CrossRefGoogle ScholarPubMed
Vythilingam, I., Lian, C.G. & Thim, S. (1992) Evaluation of carbon dioxide and 1-octen-3-ol as mosquito attractants. Southeast Asian Journal of Tropical Medicine and Public Health 23, 328–332.Google Scholar
Zinser, M., Ramberg, F. & Willott, E. (2004) Culex quinquefasciatus (Diptera: Culicidae) as a potential West Nile virus vector in Tuscon, Arizona: blood meal analysis indicates feeding on both humans and birds. Journal of Insect Science 4, 20.Google Scholar