Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-28T20:43:11.500Z Has data issue: false hasContentIssue false
  • English
  • Français

Electrophoretic study of enzymes from cereal aphid populations. II. Use of electrophoresis for identifying aphidiid parasitoids (Hymenoptera) of Sitobion avenae (F.) (Hemiptera: Aphididae)

Published online by Cambridge University Press:  10 July 2009

P. Castañera ,
H. D. Loxdale  and
Kathy Nowak
P. Castañera
Affiliation:
Entomology Department, Rothamsted Experimental Station, Harpenden, Herts., AL5 2JQ, UK
H. D. Loxdale
Affiliation:
Entomology Department, Rothamsted Experimental Station, Harpenden, Herts., AL5 2JQ, UK
Kathy Nowak
Affiliation:
Entomology Department, Rothamsted Experimental Station, Harpenden, Herts., AL5 2JQ, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

The effect of parasitism by the aphidiid wasp Aphidius uzbekistanicus (Luzhetski) on the isoenzyme banding patterns of 14 soluble enzymes separated by one–dimensional slab polyacrylamide gel electrophoresis from crude homogenates of its main host, Sitobion avenae (F.), are described. Parasitism was found to be detectable using any 12 of these enzymes. The differences observed between the banding patterns for three enzymes (ester–ase, malate dehydrogenase and malic enzyme) separated from the adults of five primary parasitoid species attacking this aphid (A. picipes (Nees), A. uzbekistanicus, Ephedrus plagiator (Nees) Praon volucre (Hal.) and Toxares deltiger (Hal.)) are also described. Esterase was found to be the best enzyme for taxonomic differentiation of all aphidiid species examined. The use of electrophoresis for assessing percentage wasp parasitism in integrated management studies of cereal aphids is discussed.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 73 , Issue 4 , December 1983 , pp. 659 - 665
Copyright
Copyright © Cambridge University Press 1983

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Dean, G.Dewar, A. M.Powell, W.Wilding, N. (1980). Integrated control of cerealaphids. — Bull. SROP 3 (4), 30–47.Google Scholar
Dean, G. J.Jones, M. G.Powell, W. (1981). The relative abundance of the hymenopterous parasites attacking Metopolophium dirhodum (Walker) and Sitobion avenae (F.) (Hemiptera: Aphididae) on cereals during 1973–79 in southern England. — Bull. ent. Res. 71, 307–315.CrossRefGoogle Scholar
Furk, C.Powell, D. F.Heyd, S. (1980). Pirimicarb resistance in the melon and cotton aphid, Aphis gossypü Glover. — Pl. Path. 29, 191–196.CrossRefGoogle Scholar
George, K. S.Gair, R. (1979). Crop loss assessment on winter wheat attacked by the grain aphid, Sitobion avenae (F.), 1974–77. — Pl. Path. 28, 143–149.CrossRefGoogle Scholar
Graham-Bryce, I. J.Holloman, D. W.Lewis, T. (1979). Pest and disease control in cereals: a research viewpoint. — Jl R. agric. Soc. 140, 131–139.Google Scholar
Hagen, K. S.Van Den Bosch, R. (1968). Impact of pathogens, parasites, and predators on aphids. — A. Rev. Ent. 13, 325–384.CrossRefGoogle Scholar
Harris, H.Hopkinson, D. A. (1977). Handbook of enzyme electrophoresis in human genetics. — Amsterdam, North-Holland.Google Scholar
Houk, E. J.Griffiths, G. W. (1980). Intracellular symbiotes of the Homoptera. — A. Rev. Ent. 25, 161–187.CrossRefGoogle Scholar
Loxdale, H. D.Castañera, P.Brookes, C. P. (1983). Electrophoretic study of enzymes from cereal aphid populations. I. Electrophoretic techniques and staining systems for characterising isoenzymes from six species of cereal aphids (Hemiptera: Aphididae). — Bull. ent. Res. 73, 645–657.CrossRefGoogle Scholar
Muir, R. C. (1979). Insecticide resistance in damson-hop aphid, Phorodon humuli in commercial hop gardens in Kent. — Ann. appl. Biol. 92, 1–9.CrossRefGoogle Scholar
Needham, P. H.Devonshire, A. L. (1975). Resistance to some organophosphorus insecticides in field populations of Myzus persicae from sugar beet in 1974. — Pestic. Sci. 6, 547–551.CrossRefGoogle Scholar
Powell, W. (1980). Toxares deltiger (Haliday) (Hymenoptera: Aphidiidae) parasitising the cereal aphid, Metopolophium dirhodum (Walker) (Hemiptera: Aphididae), in southern England: a new host-parasitoid record. — Bull. ent. Res. 70, 407–409.CrossRefGoogle Scholar
Powell, W. (1982). The identification of hymenopterous parasitoids attacking cereal aphids in Britain. — Syst. Entomol. 7, 465–473.CrossRefGoogle Scholar
Powell, W.Dean, G. J.Dewar, A.Wilding, N. (1981). Towards integrated control of cereal aphids. — 201–206 in 1981 British Crop Protection Conference: Pests and Diseases (11th British Insecticide and Fungicide Conference). Volume1. — pp. 1–362. Croydon, UK, Br. Crop Prot. Coun.Google Scholar
Powell, W.Dean, G. J. W.Dewar, A. M.Wilding, N.Bardner, R.Fletcher, K. E.Lofty, J. R.Edwards, C. A.Stephenson, J. W. (1980). Pests of winter wheat. — Rep. Rothamsted exp. Stn.1979(1), 87–88.Google Scholar
Starý, P. (1976). Aphid parasites (Hymenoptera, Aphidiidae) of the Mediterranean area. — 95 pp. The Hague, Junk.CrossRefGoogle Scholar
Starý, P. (1981). Biosystematic synopsis of parasitoids on cereal aphids in the western Palaearctic (Hymenoptera, Aphidiidae; Homoptera, Aphidoidea). — Ada entomol. bohemoslov. 78, 382–396.Google Scholar
Stribley, M. F.Moores, G. D.Devonshire, A. L.Sawicki, R. M. (1983). Application of the FAO-recommended method for detecting insecticide resistance in Aphis fabae Scopoli, Sitobion avenae (F.), Metopolophium dirhodum (Walker) and Rhopalosiphum padi (L.) (Hemiptera: Aphididae). — Bull. ent. Res. 73, 107–115.CrossRefGoogle Scholar
Tomiuk, J.Wöhrmann, K. (1980). Population growth and population structure of natural populations of Macrosiphum rosae (L.) (Hemiptera, Aphididae). — Z. angew. Ent. 90, 464–473.CrossRefGoogle Scholar
Tomiuk, J.Wöhrmann, K.Eggers-Schumacher, H. A. (1979). Enzyme patterns as a characteristic for the identification of aphids. — Z. angew. Ent. 88, 440–446.CrossRefGoogle Scholar
Vickerman, G. P.Wratten, S. D. (1979). The biology and pest status of cereal aphids (Hemiptera: Aphididae) in Europe: a review. — Bull. ent. Res. 69, 1–32.CrossRefGoogle Scholar
Wool, D.Van Emden, H. F.Bunting, S. W. (1978). Electrophoretic detection of the internal parasite, Aphidius matricariae in Myzus persicae. — Ann. appl. Biol. 90, 21–26.CrossRefGoogle Scholar