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The detection of predation by Abax parallelepipedus and Pterostichus madidus (Coleoptera: Carabidae) on Mollusca using a quantitative Elisa

Published online by Cambridge University Press:  10 July 2009

W. O. C. Symondson  and
J. E. Liddell
W. O. C. Symondson*
Affiliation:
School of Pure and Applied Biology and Department of Biochemistry, University of Wales College of Cardiff, UK
J. E. Liddell
Affiliation:
Department of Biochemistry, University of Wales College of Cardiff, UK
*
Dr W. O. C. Symondson, School of Pure and Applied Biology, PO Box 915, Cardiff CF1 3TL, UK.
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Abstract

The potential of carabid beetles as natural control agents of slugs was investigated using a quantitative indirect enzyme-linked immunosorbent assay (ELISA). The crop contents of two species, Abax parallelepipedus (Piller & Mitterpacher) and Pterostichus madidus (Fabricius) collected between May and December 1990, were analysed using an anti-mollusc haemolymph antiserum. The mass, immunological reaction and calculated mollusc content of each beetle crop was determined. Mollusc content was calculated as ‘fresh mollusc equivalent’, and the probable quantities of degraded material present are discussed in relation to predator and prey species. 89.5% of A. parallelepipedus and 42% of P. madidus were found to contain mollusc proteins. Although approximately the same proportion of male and female A. parallelepipedus tested positive, females contained greater quantities of mollusc remains. Approximately 39% of male and 45% of female P. madidus tested positive, and overall female crops contained significantly more material. The calculated amount of mollusc remains found in females was also greater. Over time, the immunological reactivity of A. parallelepipedus crop samples varied significantly. However, when crop weight was taken into consideration, the calculated quantity of mollusc found in strongly reacting samples was not significantly different between months in either species. Neither the immunological response nor the quantity of mollusc remains varied over time in P. madidus, although significant differences were found in overall crop weights. A significant correlation was found between the proportion of mollusc in beetle crops and crop mass in A. parallelepipedus, but not in P. madidus. Correlations between soil temperatures and crop mass, immunological reactivity and mollusc content were not significant in either species. The improved methods of quantifying predation from ELISA data, employed in this study, were an important part of a larger on-going investigation of the role of predation in slug population dynamics within agricultural systems.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 83 , Issue 4 , December 1993 , pp. 641 - 647
Copyright
Copyright © Cambridge University Press 1993

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References

Bless, Von R. (1977) Untersuchungen zur Frage des Räuber-Beute-Verhältnisses von Carabiden und Gastropoden. Anzeiger fuer Schädlingskunde, Pflanzenschutz, Umweltschutz 50, 55–57.CrossRefGoogle Scholar
Cheeson, J. (1984) Effect of notonectids (Hemiptera: Notonectidae) on mosquitoes (Diptera: Culicidae): predation or selective oviposition? Environmental Entomology 13, 531–538.CrossRefGoogle Scholar
Cruze, E. & Weldon, J. (1989) Minitab reference manual. Release 7, 348 pp. State College, PA 16801, USA, Minitab Inc.Google Scholar
Davies, M.J. (1953) The contents of the crops of some British carabid beetles. Entomologist's Monthly Magazine 89, 18–23.Google Scholar
De Ruiter, P.C. & Ernsting, G. (1987) Effect of ration on energy allocation in a carabid beetle. Functional Ecology 1, 109–116.Google Scholar
Ernsting, G. & Huyer, F.A. (1984) A laboratory study on temperature relations of egg production and development in two related species of carabid beetle. Oecologia (Berlin) 62, 361–367.Google Scholar
Ernsting, G. & van der Werf, D.C. (1988) Hunger, partial consumption of prey and prey size choice preference in a carabid beetle. Ecological Entomology 13, 155–164.Google Scholar
Faasch, H. (1968) Beobachtungen zur Biologie und zum Verhalten von Cincindela hybrida L. und Cincindela campestris L. und experimentelle Analyse ihres Beutefangverhaltens. Zoologische Jahrbuecher, Abteilung fuer Systematic Oekologie und Geographie der Tiere 95, 477–522.Google Scholar
Filliben, J.J. (1975) The probability plot correlation coefficient test for normality. Technometrics 17, 111–117.Google Scholar
Forsythe, T.G. (1982) Feeding mechanisms of certain ground beetles (Coleoptera: Carabidae). Coleopterists Bulletin 36, 26–73.Google Scholar
Fry, J.C. (1993) Bivariate regression. pp. 81–125in Fry, J.C. (Ed.) Biological data analysis a practical approach. Oxford, Oxford University Press.Google Scholar
Greene, A. (1975) Biology of five species of Cychrini (Coleoptera, Carabidae) in the steppe region of southwestern Washington. Melanderia 19, 1–43.Google Scholar
Greenslade, P.J.M. (1964) Pitfall trapping as a method for studying populations of Carabidae (Coleoptera). Journal of Animal Ecology 33, 301–310.CrossRefGoogle Scholar
Gruntal, S. Yu. & Sergeyeva, T.K. (1989) Food relation characteristics of the beetles of the genera Carabus and Cychrus. Zoologichesku Zhurnal 58, 45–51.Google Scholar
Halsall, N.B. & Wratten, S.D. (1988) The efficiency of pitfall trapping for polyphagous predatory Carabidae. Ecological Entomology 13, 293–299.CrossRefGoogle Scholar
Heessen, H.J.L. (1980) Egg production of Pterostichus oblongopunctatus (Fabricius) (Col, Carabidae) and Philonthus decorus (Gravenhorst) (Col., Staphylinidae). Netherlands Journal of Zoology 30, 35–53.Google Scholar
Loreau, M. (1984) Experimental study of the feeding of Abax ater Villers Carabus problematicus Herbst and Cychrus attenuatus Fabricius (Coleoptera Carabidae). Annales, Société Royale Zoologique de Belgique 114, 227–240.Google Scholar
Luff, M.L. (1974) Adult and larval feeding habits of Pterostichus madidus (F.) (Coleoptera: Carabidae). Journal of Natural History 8, 403–409.Google Scholar
Mols, P.J.M. (1988) Simulation of hunger, feeding and egg production in the carabid beetle Pterostichus coerulescens L. ( = Poecilus versicolor Sturm). Agricultural University Wageningen Papers 88–3, The Netherlands, Agricultural University Wageningen.Google Scholar
Murdoch, W.W., Chesson, J. & Chesson, P.L. (1985) Biological control in theory and practice. American Naturalist 125, 344–366.CrossRefGoogle Scholar
Rohlf, F.J. & Sokal, R.R. (1981) Statistical tables. 2nd edn.219 PP. San Francisco, W.H. Freeman and Company.Google Scholar
Siegel, S. (1956) Nonparametric statistics for behavioural sciences. 312 pp. McGraw-Hill series in Psychology. London, McGraw-Hill Book Company.Google Scholar
Sopp, P.I. & Sunderland, K.D. (1989) Some factors affecting the detection period of aphid remains in predators using ELISA Entomologia Experimentalis et Applicata 51, 11–20.CrossRefGoogle Scholar
Sokal, R.R. & Rohlf, F.J. (1987) Introduction to biostatistics. 2nd edn.363 pp. New York, W. H. Freeman and Company.Google Scholar
Stephenson, J.W. (1968) A review of the biology and ecology of slugs of agricultural importance. Proceedings of the Malacological Society of London 38, 169–178.Google Scholar
Symondson, W.O.C. (1989) Biological control of slugs by carabids. pp. 295–300in Henderson, I. (Ed.) Slugs and snails in world agriculture. Thornton Heath, British Crop Protection Council (BCPC Monograph 41).Google Scholar
Symondson, W.O.C. (1992) The biological control of slugs by carabid beetles. PhD thesis, University of Wales College of Cardiff.Google Scholar
Symondson, W.O.C. (in press) The effects of crop development upon slug distribution and control by Abax parallelepipedus (Coleoptera: Carabidae). Annals of Applied Biology 123.Google Scholar
Symondson, W.O.C. & Liddell, J.E. (1993) A monoclonal antibody for the detection of arionid slug remains in carabid predators. Biological Control 3, 207–214.Google Scholar
Symondson, W.O.C. & Liddell, J.E. (in press a) Differential antigen decay rates during digestion of molluscan prey by carabid predators. Entomologia Experimentalis et Applicata 69.Google Scholar
Symondson, W.O.C. & Liddell, J.E. (in press b) The development and characterisation of an anti-haemolymph antiserum for the detection of mollusc remains within polyphagous carabids (Coleoptera: Carabidae). Biocontrol Science and Technology 3.Google Scholar
Tod, M.E. (1970) The significance of predation by soil invertebrates on field populations of Agriolimax reticulatus (Gastropoda, Limacidae). PhD thesis, University of Edinburgh.Google Scholar
Tod, M.E. (1973) Notes on beetle predators of molluscs. Entomologist 106, 196–201.Google Scholar
Wheater, C.P. (1989) Prey detection by some predatory Coleoptera (Carabidae and Staphylinidae). Journal of Zoology, London 218, 171–185.CrossRefGoogle Scholar