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Detection of esterase activity in susceptible and organophosphate resistant strains of the cattle tick Boophilus microplus (Acari: Ixodidae)

Published online by Cambridge University Press:  10 July 2009

R. Rosario-Cruz ,
E. Miranda-Miranda ,
Z. Garcia-Vasquez  and
M. Ortiz-Estrada
R. Rosario-Cruz
Affiliation:
Department of Veterinary Pathobiology, Texas A & M University, College Station, Texas 77843–4467, USA
E. Miranda-Miranda
Affiliation:
Cenid-Parasitologia Veterinaria INIFAP-SARH, Apartado Postal 206 Civac, Morelos, CP 62500, Mexico
Z. Garcia-Vasquez
Affiliation:
Cenid-Parasitologia Veterinaria INIFAP-SARH, Apartado Postal 206 Civac, Morelos, CP 62500, Mexico
M. Ortiz-Estrada
Affiliation:
Direccion General de Salud Animal-SARH, Centro Nacional de Parasitologia Animal, Carretera Federal Cuernavaca-Cuautla km 12.5, Jiutepec, Morelos, Mexico
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Abstract

Two organophosphate (OP) resistant strains of the cattle tick Boophilus microplus (Canestrini) from Mexico and Costa Rica were used to analyse the presence of esterase activity associated with resistance. The concentrations of six major proteins in both resistant strains were increased compared to the susceptible Morelos strain, both when stained with Coomassie Brilliant Blue after SDS-PAGE, and when analysed for esterase activity by the hydrolysis of naphthyl acetate esters. Esterases were named A or B in relation to the substrate preference for alpha or beta naphthyl acetate and numbered according to their position on the SDS—PAGE. The molecular weights of these proteins were: 125, 115, 108, 77, 43 and 67 Kd for Est-Bl, Est-B2, Est-B3, Est-B4, Est-B5 and Est-A respectively. Est-B3 showed cholinesterase (ChE) activity. This study strengthens the hypothesis that the mechanism associated with OP resistance found in many other insects includes an increase of esterase activity, probably as a result of gene amplification. The genes encoding these enzymes could be potentially used as molecular markers to detect resistance in the cattle tick B. microplus using a DNA probe.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 87 , Issue 2 , April 1997 , pp. 197 - 202
Copyright
Copyright © Cambridge University Press 1997

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