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Classification of tsetse flies Glossina spp. (Diptera: Glossinidae) by gas chromatographic analysis of cuticular components

Published online by Cambridge University Press:  10 July 2009

D.A. Carlson ,
S.K. Milstrey  and
S.K. Narang
D.A. Carlson*
Affiliation:
USDA, ARS, Medical and Veterinary Entomology Research Laboratory, Gainesville, Florida, USA*
S.K. Milstrey
Affiliation:
USDA, ARS, Medical and Veterinary Entomology Research Laboratory, Gainesville, Florida, USA*
S.K. Narang
Affiliation:
USDA, ARS, Biosciences Research Laboratory, Fargo, North Dakota, USA
*
Dr D.A. Carlson, USDA, ARS, MAVERL, PO Box 14565, Gainesville, FL 32604, USA.
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Abstract

Gas liquid chromatography (GC) was used to analyse the cuticular alkanes of 26 species and subspecies of tsetse flies (Glossina spp.). Unique interspecific and intraspecific (males vs. females) chromatographic patterns were observed. Solvent extraction of dried museum specimens and fresh specimens were equally successful, and left specimens undamaged. GC peaks were used as characters with one of five character states per peak, to show phenetic relationships among species by sex using a UPGMA algorithm, using 23 peaks for males and 40 peaks for females. Comparisons among species of the morsitans group often agreed with recognized morsitans group classification using morphological techniques, with G. austeni Newstead being somewhat aberrant. Glossina tachinoides Westwood was less closely related to others in the palpalis group than previously described using morphology. Eleven members of the rarely studied fusca group were also classified by this methodology.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 83 , Issue 4 , December 1993 , pp. 507 - 515
Copyright
Copyright © Cambridge University Press 1993

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