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Aldehyde oxidases mediate plant toxicant susceptibility and fecundity in the red flour beetle, Tribolium castaneum

Published online by Cambridge University Press:  16 February 2022

Yonglei Zhang ,
Jiahao Zhang ,
Dongyu Li ,
Haidi Sun ,
Ruixue Lu ,
Se Yin ,
Xinlong Guo  and
Shanshan Gao Open the ORCID record for Shanshan Gao [Opens in a new window]
Yonglei Zhang
Affiliation:
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China
Jiahao Zhang
Affiliation:
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China
Dongyu Li
Affiliation:
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China
Haidi Sun
Affiliation:
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China
Ruixue Lu
Affiliation:
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China
Se Yin
Affiliation:
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China
Xinlong Guo
Affiliation:
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China
Shanshan Gao*
Affiliation:
College of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China
*
Author for correspondence: Shanshan Gao, Email: [email protected]
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Abstract

Aldehyde oxidases (AOXs) are a group of metabolic enzymes that play critical roles in the degradation of xenobiotics and chemicals. However, the physiological function of this enzyme in insects remains poorly understood. In this study, three TcAOX genes (TcAOX1, TcAOX2, TcAOX3) were identified and characterized from Tribolium castaneum genome. Spatiotemporal expression profiling showed that TcAOX1 expression was most highly expressed at the early pupal stage and was predominantly expressed in the antennae of adults, indicating that TcAOX1 was involved in the degradation of chemical signals; TcAOX2 expression was most highly expressed at the late pupal stage and was mainly expressed in the fat body, epidermis of larvae and adults, respectively; and TcAOX3 expression was in all stages and was primarily expressed in the head of adults. Moreover, the transcripts of TcAOX2 and TcAOX3 were significantly induced after exposure to plant oil, and RNA interference (RNAi) targeting of each of them enhanced the susceptibility of beetles to this plant toxicant, suggesting that these two genes are associated with plant toxicant detoxification. Intriguingly, knockdown of the TcAOX1 led to reductions in female egg-laying but unchanged the hatchability and the development of genital organs, suggesting that this gene may mediate fecundity by effecting the inactivation of chemical signals in T. castaneum. Overall, these results shed new light on the function of AOX genes in insects, and could facilitate the development of research on pest control management.

Keywords

Aldehyde oxidasesfecundityplant toxicant susceptibilityRNA interferenceTribolium castaneum
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 112 , Issue 5 , October 2022 , pp. 656 - 666
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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