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Acute, sublethal and combination effects of azadirachtin and Bacillus thuringiensis toxins on Helicoverpa armigera (Lepidoptera: Noctuidae) larvae

Published online by Cambridge University Press:  24 July 2007

G. Singh ,
P. J. Rup  and
Opender Koul
G. Singh
Affiliation:
Insect Biopesticide Research Centre, 30 Parkash Nagar, Jalandhar 144 003, India
P. J. Rup
Affiliation:
Department of Zoology, Guru Nanak Dev University, Amritsar 143 005, India
Opender Koul*
Affiliation:
Insect Biopesticide Research Centre, 30 Parkash Nagar, Jalandhar 144 003, India
*
*Fax: 181-2274336 E-mail: [email protected]
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Abstract

The efficacy of neem (1500 ppm azadirachtin (AI)), Delfin®WG™, a biological insecticide based on selected strain of Bacillus thuringiensis Berliner (Bt) subspecies kurstaki, and Cry1Ac protein, either individually or in combination, were examined against first to fourth instar Helicoverpa armigera (Hübner) larvae. Using an oral administration method, various growth inhibitory concentrations (EC) and lethal concentrations (LC) were determined for each bioagent. Combinations of sublethal concentrations of Bt spray formulation with azadirachtin at EC50 or EC95 levels not only enhanced the toxicity, but also reduced the duration of action when used in a mixture. The LC20 and LC50 values for Cry1Ac toxin were 0.06 and 0.22 μg ml−1, respectively. Bt–azadirachtin combinations of LC50+EC20 and LC50+EC50 result in 100% mortality. The mortality also was significant in LC20+EC20 and LC20+EC50 mixtures. These studies imply that the combined action is not synergistic but complimentary, with azadirachtin particularly facilitating the action of Bt. The Bt spray–azadirachtin combination is more economical than combinations that involve isolating the toxic protein, as the Bt spray formulations can be combined in a spray mixture with neem. These combinations may be useful for controlling bollworm populations that have acquired resistance to Bt as they may not survive the effect of mixture. Azadirachtin may be useful as a means of reducing the endotoxin concentrations in a mixture, to promote increased economic savings and further reduce the probability of resistance development to either insect control agent.

Keywords

bollwormneemBt toxinscontrolazadirachtinHelicoverpa armigera
Type
Research Article
Information
Bulletin of Entomological Research , Volume 97 , Issue 4 , August 2007 , pp. 351 - 357
Copyright
Copyright © Cambridge University Press 2007

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References

Altre, J.A., Grafius, E.J. & Whalon, M.E. (1996) Feeding behaviour of CryIIIA-resistant and susceptible Colorado potato beetle (Coleoptera: Chrysomelidae) larvae on Bacillus thuringiensis tenebrionis-transgenic CryIIIA treated and untreated potato foliage. Journal of Economic Entomology 89, 311–317.CrossRefGoogle Scholar
Alves, A.P., Spencer, T.A., Tabashnik, B.E. & Siegfried, B.D. (2006) Inheritance of resistance to the Cry1Ab Bacillus thuringiensis toxin in Ostrinia nubilalis (Lepidoptera: Crambidae). Journal of Economic Entomology 99, 494–501.CrossRefGoogle Scholar
Bradford, M.M. (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 74, 248–258.CrossRefGoogle Scholar
Champagne, D.E., Koul, O., Isman, M.B., Scudder, G.E. & Towers, G.H.N. (1992) Biological activity of limonoids from the rutales. Phytochemistry 31, 377–394.CrossRefGoogle Scholar
Devaki, K. & Krishnayya, P.V. (2004) Combination effects of three proprietary formulations of B.t. with neem against Spodoptera litura (Fab.). Indian Journal of Plant Protection 32, 34–36.Google Scholar
Ferre, J. & van Rie, J. (2002) Biochemistry and genetics of insect resistance to Bacillus thuringiensis. Annual Review of Entomology 47, 501–533.CrossRefGoogle ScholarPubMed
Finney, D.J. (1971) Probit analysis. 350 pp. Cambridge, Cambridge University Press.Google Scholar
Gould, F.A., Anderson, A., Jones, A., Sumerford, D., Heckel, D.G., Lopez, J., Micinski, S., Leonard, R. & Laster, M. (1997) Initial frequency of alleles for resistance to Bacillus thuringiensis toxins in field populations of Heliothis virescens. Proceedings of the National Academy of Sciences, USA 94, 3519–3523.CrossRefGoogle ScholarPubMed
Govindachari, T.R., Narasimhan, N.S., Suresh, G., Partho, P.D., Gopalakrishnan, G. & Krishnakumari, G.N. (1995) Structure-related insect antifeedant and growth regulating activities of some limonoids. Journal of Chemical Ecology 21, 1585–1600.CrossRefGoogle ScholarPubMed
Gujar, G.T., Kumari, A., Kallia, V. & Chandrashekar, K. (2000) Spatial and temporal variation in susceptibility of the American bollworm, Helicoverpa armigera to Bacillus thuringiensis var. kurstaki in India. Current Science 78, 995–1001.Google Scholar
Janmaat, A.F. & Myers, J.H. (2003) Rapid evolution and the cost of resistance to Bacillus thuringiensis in greenhouse populations of cabbage loopers, Trichoplusia ni. Proceedings of the Royal Society of London, B 270, 2263–2270.CrossRefGoogle ScholarPubMed
Koul, O. (2005) Insect antifeedants. 1005 pp. Boca Raton, Florida, CRC Press.Google Scholar
Koul, O. & Wahab, S. (2004) Neem: today and in the new millennium. 276 pp. Netherlands, Kluwer Academic Publishers.CrossRefGoogle Scholar
Koul, O., Smirle, M.J. & Isman, M.B. (1990) Asarones from Acorus calamus L. oil: their effect on feeding behaviour and dietary utilization in Peridroma saucia. Journal of Chemical Ecology 16, 1911–1920.CrossRefGoogle Scholar
Koul, O., Shankar, J.S. & Kapil, R.S. (1996) The effect of neem allelochemicals on nutritional physiology of larval Spodoptera litura. Entomologia Experimentalis et Applicata 79, 43–50.CrossRefGoogle Scholar
Koul, O., Shankar, J.S., Mehta, N., Taneja, S.C., Tripathi, A.K. & Dhar, K.L. (1997) Bioefficacy of crude extracts of Aglaia species (Meliaceae) and some active fractions against lepidopteran larvae. Journal of Applied Entomology 121, 245–248.CrossRefGoogle Scholar
Koul, O., Multani, J.S., Goomber, S., Daniewski, W.M. & Berlozecki, S. (2004) Activity of some non-azadirachtin limonoids from Azadirachta indica against lepidopteran larvae. Australian Journal of Entomology 43, 189–195.CrossRefGoogle Scholar
Lee, M.K., Milne, A. & Dean, D.H. (1992) Location of Bombyx mori receptor binding region of a Bacillus thuringiensis δ-endotoxin. Journal of Biological Chemistry 267, 3115–3121.CrossRefGoogle ScholarPubMed
Lowery, D.T. & Isman, M.B. (1994) Insect growth regulating effects of neem extract and azadirachtin on aphids. Entomologia Experimentalis et Applicata 72, 77–84.CrossRefGoogle Scholar
Moar, W.J. & Trumble, J.T. (1987) Toxicity, joint action, and mean time of mortality of Dipel 2X, avermectin B1, neem, and thuringiensis against beet armyworms (Lepidoptera: Noctuidae). Journal of Economic Entomology 80, 588–592.CrossRefGoogle Scholar
Murray, K.D., Alford, A.R., Groden, E., Drummond, F.A., Storch, R.H., Bentley, M.D. & Sugathapala, P.M. (1993) Interactive effects of an antifeedant used with Bacillus thuringiensis var. san diego delta endotoxin on Colorado potato beetle (Coleoptera: Chrysomelidae). Journal of Economic Entomology 86, 1793–1801.CrossRefGoogle Scholar
Pimentel, D. & Burgess, M. (1985) Effects of single versus combinations of insecticides on the development of resistance. Environmental Entomology 14, 582–589.CrossRefGoogle Scholar
Pree, D.J. & Daly, J.C. (1996) Toxicity of mixtures of Bacillus thuringiensis with endosulfan and other insecticides to the cotton boll worm Helicoverpa armigera. Pesticide Science 48, 199–204.3.0.CO;2-9>CrossRefGoogle Scholar
Rajyashri, K.R. & Mohan, M. (2004) Gene pyramiding: a transgenic approach to enhancing resistance durability in plants. pp. 219–260in Koul, O. & Dhaliwal, G.S (Eds) Transgenic crop protection: concepts and strategies. Enfield, New Hampshire, Science Publishers Inc.Google Scholar
Roush, R.T. (1989) Designing resistance management programs: how can you choose? Pesticide Science 26, 423–441.CrossRefGoogle Scholar
Salama, H.S., Foda, M.S., Zaki, F.N. & Moawad, S. (1984) Potency of combinations of Bacillus thuringiensis and chemical insecticides on Spodoptera littoralis (Lepidoptera: Noctuidae). Journal of Economic Entomology 77, 885–890.CrossRefGoogle Scholar
Shelton, A.M., Zhao, J.-Z. & Roush, R.T. (2002) Economic, ecological, food safety, and social consequences of the deployment of Bt transgenic plants. Annual Review of Entomology 47, 845–881.CrossRefGoogle ScholarPubMed
Sivamani, E., Rajendran, N., Senrayan, R., Ananthakrishnan, T.N. & Kunthala, J. (1992) Influence of some plant phenolics on the activity of δ-endotoxin of Bacillus thuringiensis var. galleriae on Heliothis armigera. Entomologia Experimentalis et Applicata 63, 243–248.CrossRefGoogle Scholar
SPSS, Inc. (1999) SPSS 10.0.6. Chicago, Illinois, SPSS Inc.Google Scholar
Stark, J.D. & Walter, J.F. (1995) Neem oil and neem oil components affect the efficacy of commercial neem insecticides. Journal of Agricultural and Food Chemistry 43, 507–512.CrossRefGoogle Scholar
Tabashnik, B.E. (1989) Managing resistance with multiple pesticide tactics: theory, evidence, and recommendations. Journal of Economic Entomology 82, 1263–1269.CrossRefGoogle ScholarPubMed
Tabashnik, B.E. (1994) Evolution of resistance to Bacillus thuringiensis. Annual Review of Entomology 39, 47–79.CrossRefGoogle Scholar
Tabashnik, B.E., Carriere, Y., Dennehy, T.J., Morin, S., Sisterson, M.S., Roush, R.T., Shelton, A.M. & Zhao, J.-Z. (2003) Insect resistance to transgenic Bt crops: lessons from the laboratory and field. Journal of Economic Entomology 96, 1031–1038.CrossRefGoogle ScholarPubMed
Trisyono, A. & Whalon, M.E. (1997) Fitness costs of resistance to Bacillus thuringiensis in Colorado potato beetle (Coleoptera: Chrysomelidae). Journal of Economic Entomology 90, 267–271.CrossRefGoogle Scholar
Trisyono, A. & Whalon, M.E. (1999) Toxicity of neem applied alone and in combination with Bacillus thuringiensis to Colorado potato beetle. Journal of Economic Entomology 92, 1281–1288.CrossRefGoogle Scholar
Wilkinson, L. (1990) Systat: the system of statistics. Evanston, Illinois, Systat Inc.Google Scholar