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Principles of techniques that rely on gas measurement in ruminant nutrition

Published online by Cambridge University Press:  27 February 2018

M. K. Theodorou ,
R. S. Lowman ,
Z. S. Davies ,
D. Cuddeford  and
E. Owen
M. K. Theodorou
Affiliation:
Department of Animal Science and Microbiology, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB
R. S. Lowman
Affiliation:
Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Roslin, Midlothian EH25 9RG
Z. S. Davies
Affiliation:
Department of Animal Science and Microbiology, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB
D. Cuddeford
Affiliation:
Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Roslin, Midlothian EH25 9RG
E. Owen
Affiliation:
Department of Agriculture, University of Reading, Earley Gate, Reading RG6 2AT
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Abstract

In vitro gas production techniques have become popular for characterizing the rate and extent of digestion of ruminant foods. In a typical gas production study, gas (predominantly carbon dioxide) is produced as particles of substrate are fermented by rumen micro-organisms in a bicarbonate buffered culture medium. Innovations in equipment design, including automated pressure recording systems and mathematical descriptions of the gas production profiles themselves, make the techniques both simple and precise and therefore good as laboratory procedure. The technique of measuring gas is of value in ruminant science because the kinetics of gas production and substrate degradation are very closely correlated. However, although it is relatively easy to measure gas volumes and to determine the kinetics of gas production, the underlying processes that give rise to the gas in the first place are complex and not well understood. Therefore, there is concern about what is being measured in gas production studies and how this relates to the digestion process in the ruminant animal. In this paper we review some of the fundamental properties of gases, describe their behaviour in liquids and consider some of the biological and chemical factors influencing gas production. From a knowledge of how gases behave in liquids and at gas-liquid interfaces, both at ambient and increased temperatures and pressures, it is possible to deduce what is happening in gas production studies. However, although the technique is invaluable for obtaining information about the digestion of particulate substrates in anaerobic ecosystems, we conclude that gas production should be used with caution in routine food evaluation studies.

Type
In vitro techniques for measuring rumen microbial activity
Information
BSAP Occasional Publication , Volume 22: In vitro techniques for measuring nutrient supply to ruminants , 1998 , pp. 55 - 63
Copyright
Copyright © British Society of Animal Science 1998

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References

Atkins, P. W. 1992. The elements of physical chemistry. Oxford University Press, Great Britain.Google Scholar
Atkins, P. W. and Clugston, T. 1987. Principles of physical chemistry. Longman, Great Britain.Google Scholar
Beuvink, J. M. W. and Spoelstra, S. F. 1992. Interactions between substrate, fermentation end-products, buffering systems and gas production upon fermentation of different carbohydrates by mixed rumen micro-organisms in vitro . Applied Microbiology and Biotechnology 37: 505–509.CrossRefGoogle Scholar
Beuvink, J. M. W. and Kogutk, J. 1993. Modelling gas production kinetics of grass silages incubated with buffered rumen fluid. Journal of Animal Science 71:1041–1046.CrossRefGoogle Scholar
Blummel, M. and Ørskov, E. R. 1993. Comparison of an in vitro gas production and nylon bag degradability of roughages in predicting feed intake in cattle. Animal Feed Science and Technology 40: 109–119.CrossRefGoogle Scholar
Chang, R. 1981. Physical chemistry with applications to biological system. Macmillan, New York.Google Scholar
Cone, J. W. 1997. The development, use and application of the gas production technique at ID-DLO, The Netherlands. In In vitro techniques for measuring nutrient supply to ruminants(ed. Deaville, E. R., Owen, E., Adesogan, A. T., Rymer, C., Huntington, J. A. and Lawrence, T. L. J.), pp. 00–00. British Society of Animal Science occasional publication no.22.Google Scholar
Davies, D. R. 1991. Growth and survival of anaerobic fungi in batch culture and in the digestive tract of ruminants. Ph.D. thesis, University of Manchester.Google Scholar
Davies, D. R., Theodorou, M. K., Baughan, J., Brooks, A. E. and Newbold, J. R. 1995. An automated pressure evaluation system (APES) for determining the fermentation characteristics of ruminant feeds. Annates de Zootechnie, 44: 36.CrossRefGoogle Scholar
Demayer, D. and Giesecke, D. 1973. Addau der Kohlenhydrate und Biochemie der Garung in Pansen. In Biologie und Biochemie der mikrobiellen Verdauung(ed. Giesecke, D. and Henderickx, H. K.), pp. 135–167. BLV Verlagsgesellschaft, Munchen.Google Scholar
France, J., Dhanoa, M. S., Theodorou, M. K., Lister, S. J. Davies, D. R. and Isaac, D. 1993. A model to interpret gas accumulation profiles associated with in vitro degradation of ruminant feeds. Journal of Theoretical Biology 163: 99–111.CrossRefGoogle Scholar
Groot, J. C. J., Cone, J .W., Williams, B. A., Debersaque, F. and Lantinga, E. A. 1996. Multiphasic analysis of gas production kinetics on in vitro ruminal fermentation. Animal Feed Science and Technology 64: 77–89. CrossRefGoogle Scholar
Hungate, R. E. 1966.The rumen and its microbes. Academic Press, New York.Google Scholar
Khan, I., Brimblecombe, P. and Clegg, S. L. 1995. Solubilities of pyruvic acid and the lower (C1-C6) carboxylic acids. Experimental determination of equilibrium vapour pressures above pure aqueous and salt solutions. Journal ofAtmospheric Chemistry 22: 285–302.CrossRefGoogle Scholar
Lowe, S. L., Theodorou, M. K., Trinci, A. P. J. and Hespell, R. B. 1985. Growth of anaerobic rumen fungi on defined and semi-defined media lacking rumen fluid. Journal of General Microbiology 131: 2225–2229.Google Scholar
Lowenadler, J. and Ronner, U. 1994. Determination of dissolved carbon-dioxide by coulometric titration in modified atmosphere systems. Letters in Applied Microbiology 18: 285–288.CrossRefGoogle Scholar
McDonald, P., Edwards, R. A., Greenhaugh, J. F. D. and Morgan, C. A. 1995. Animal Nutrition, fifth edition. Longman, Singapore Google Scholar
Meier-Schneiders, M., Schafer, F., Grosshans, U. and Busch, C. 1995. Impact of carbon-dioxide evolution on the calorimetric monitoring of fermentations. Thermochimica Acta 251: 85–97.CrossRefGoogle Scholar
Menke, K., Raab, L., Salewski, A., Steingass, H., Fritz, D. and Schneider, W. 1979. The estimation of digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro . Journal of Agricultural Science, Cambridge 3: 217–222.CrossRefGoogle Scholar
Morris, J. G. 1983. A biologist's physical chemistry, second edition. Edward Arnold, Great Britain.Google Scholar
Nielsen, B. B. 1996. A study of survival, growth and enzyme production in anaerobic fungi. Ph.D. thesis, University of Manchester.Google Scholar
Nyiri, L. and Lengyel, Z. L. 1968. Studies on ventilation of culture broths. 1. Behaviour of C02 in model systems. Biotechnology and Bioengineering 10: 133–150.CrossRefGoogle Scholar
Opatpatanakit, Y., Kellaway, R., Lean, I. J., Annison, G. and Kirby, A. 1994. Microbial fermentation of cereal grains in vitro. Australian Journal of Agricultural Research 45: 1247–1263.CrossRefGoogle Scholar
Pell, A. N. and Schofield, P. 1993. Nutrition, feeding and calves. Computerised monitoring of gas production to measure forage digestion in vitro. Journal of Dairy Science 76: 1063–1073.CrossRefGoogle Scholar
Russel, J. B. and Wallace, R. J. 1996. Energy yielding and consuming reactions. In The rumen microbial ecosystem, second edition(ed. Hobson, P. N.), pp. 185–215. Elsevier Applied Science, London.Google Scholar
Sagan, C. 1981. Cosmos. Macdonald Futura Publishers.Google Scholar
Schofield, P., Pitt, R. E. and Pell, A. N. 1994. Kinetics of fibre digestion from in vitro gas production. Journal of Animal Science 72:2980–2991.CrossRefGoogle ScholarPubMed
Sileshi, Z. 1994. Development of a simple in vitro gas production technique, using a pressure transducer, to estimate rate of digestion of some Ethiopian forages. Ph.D. thesis, University of Reading.Google Scholar
Theodorou, M. K., Williams, B. A., Dhanoa, M. S., McAllan, A. B. and France, J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48: 185–197.CrossRefGoogle Scholar
Theodorou, M. K., Davies, D. R., Nielsen, B. B., Lawrence, M. I. G. and Trinci, A. P. J. 1995. Determination of the growth of anaerobic fungi on soluble and cellulosic substrates using a pressure transducer. Journal of General Microbiology 141: 671–678.Google Scholar
Van Loo, E. N., Reheul, D., Cone, J. W. and Snijders, C. H. A., 1994. Genetic variation in perennial ryegrass for gas production during in vitro rumen fermentation. In: Breeding for quality(ed. Reheul, D. and Ghesquiere, A.), pp. 35–41. Eucarpia Fodder Crop Section, Belgium.Google Scholar
Wolin, M. J. 1975. Interactions between the bacterial species of the rumen. In Digestion and metabolism in the ruminant(ed. McDonald, I. W. and Warner, A. C.). Academic Press, London.Google Scholar