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Development, validation and application of a three-step in vitro procedure for estimating intestinal digestion of protein in ruminants

Published online by Cambridge University Press:  27 February 2018

S. Calsamiglia  and
M. D. Stern
S. Calsamiglia
Affiliation:
Departamento de Patologia y Produccion Animal, Universitat Autonoma de Barcelona, 08193-Bellaterra, Spain
M. D. Stern
Affiliation:
Department of Animal Science, University of Minnesota, St Paul, MN 55108, USA
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Extract

The total amount of dietary protein available for absorption is dependent on the flow of dietary nitrogen (N) to the duodenum and its intestinal digestibility. Variation in intestinal digestion among protein supplements has been reported in vivo (Stern et al., 1985) and in situ (Hvelplund, 1985). Obtaining estimates of protein digestion in the small intestine is expensive, labour intensive and requires the use of surgically prepared animals. The objective of this study was to develop a reliable in vitro technique to estimate intestinal digestion of proteins in ruminants.

Type
Use of enzymes in in vitro methods
Information
BSAP Occasional Publication , Volume 22: In vitro techniques for measuring nutrient supply to ruminants , 1998 , pp. 127 - 129
Copyright
Copyright © British Society of Animal Science 1998

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