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The application of gene technology to the improvement of fish

Published online by Cambridge University Press:  27 February 2018

Norman Maclean  and
David Penman
Norman Maclean
Affiliation:
Department of Biology, Southampton University, England, U.K.
David Penman
Affiliation:
Department of Biology, Southampton University, England, U.K.
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Abstract

Because fish lay large numbers of eggs, in which both fertilisation and development is external, they are rather suitable organisms for transgenic induction. To date fish of many different species have been used in such work - rainbow trout, atlantic salmon, channel cat fish, tilapia, medaka, zebrafish, goldfish, carp and loach. Normally the induction of transgenics has involved injection of multiple copies of the chosen novel gene into the egg cytoplasm soon after fertilisation, followed by assays for subsequent gene integration, expression and transmission to progeny.

Genes which have been utilised for transgenic induction in fish include somatotrophin (growth hormone) of both mammalian and piscine origin, metallothionein, 'antifreeze' protein, and crytallin coding sequences. A number of laboratories have demonstrated by Southern blotting of restricted DNA fragments that genomic incorporation has been achieved, although in others there appears to be a continuing problem of long term persistance of extrachromosomal DNA material. Expression of the injected sequences tends to be at a low level and in some cases not to be detectable. This is probably due to the inefficiency of mammalian gene promoters in fish cells and will hopefully be overcome by the use of genes of piscine origin. Germ line integration and transmission to progeny of the novel sequence has been established in a few laboratories.

Transgenic fish may also offer some potential as expression systems for valuable pharmaceutical products. Problems of containment and planned release of transgenic fish are also briefly discussed.

Type
Molecular Biology and Genetic Manipulation
Information
BSAP Occasional Publication , Volume 12: Animal Breeding Opportunities , 1988 , pp. 58 - 70
Copyright
Copyright © British Society of Animal Production 1988

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References

Brem, G., Brenig, B., Horstgen-Schwark, G. and Winnacker, E.L. (1988) Gene transfer in tilapia (Oreochromis niloticus) Aquaculture 68 209–219.Google Scholar
Bye, V.J. and Lincoln, R.F. (1986) Commercial methods for the control of sexual maturation in rainbow trout (salmo gairdneri) Aquaculture 57 299–309.Google Scholar
Chourrout, D., Guyomard, R., Houdebine, L. (1986) High efficiency gene transfer in rainbow trout (Salmo gairdneri) by microinjection into egg cytoplasm. Aquaculture 51 143–150.CrossRefGoogle Scholar
Chourrout, D. and Quillet, E. (1982) Induced gyno-genesis in the rainbow trout: sex and survival of progenies. Production of all triploid populations. Theor. Appli. Genet. 63 201–205.CrossRefGoogle ScholarPubMed
Dunham, R.A., Eash, J., Askins, J., and Townes, T.M. (1987) Transfer of the metallothionein-human growth hormone fusion gene into channel catfish. Trans. Am. Fish Soc. 116 87–91.Google Scholar
Etkin, L.D. and Balcells, S. (1985) Transformed xenopus embryos as a transient expression system to analyse gene expression at the midblastula transition. Developmental Biology 108 173–178.Google Scholar
Fletcher, G.L., Kao, M.H., and Fourney, R.M. (1988) Antifreeze peptides confer freezing resistance to fish. Can. J. Zool. 64 1897–1901.CrossRefGoogle Scholar
Fletcher, G.L., Shears, M.A., King, M.J., Davies, P.L. and Hew, C.L. (1988) Evidence for antifreeze protein gene transfer in Atlantic salmon (Salmo salar) Can. J. Fisheries and Aquatic Sciences 45 352–357.Google Scholar
Gill, J.A., Sumpter, J.P., Donaldson, E.M., Dye, H.M., Souza, L., Berg, T., Wypych, J. and Langley, K. (1985). Recombinant chicken and bovine growth hormones accelerate growth in aquacultured juvenile Pacific salmon Oncorhynchus kisutch . Bio Technology 3 643–646.Google Scholar
Guyomard, R., Chourrout, U., Houdebine, L. (1988) Production of stable transgenic fish by cytoplasmic injection of purified genes. Proceedings of UCLA Symposium on Gene transfer and Gene Therapy February 1988, Colorado USA (in the press).Google Scholar
Maclean, N., Penman, D., and Zhu, Z. (1987) Introduction of novel genes into fish. Biotechnology 5 257–261.Google Scholar
Ozato, K., Kondoh, H., Inohara, H., Iwamatsu, T., Wakamatsu, Y, and Okado, T.S. (1986) Production of transgenic fish: introduction and expression of chicken crystallin gene in medaka embryos. Cell Differ. 19 237–244.Google Scholar
Palmiter, R.D., and Brinster, R.L. (1986) Germ line transformation of mice. A. Rev. Genet. 20 465–499.Google Scholar
Palmiter, R.D., Brinster, R.L., Hammer, R.E., Trumbauer, M.E., Rosenfeld, M.G., Birnberg, N.C. and Evans, R.M. (1982) Dramatic growth of mice that develop from eggs microinjected withmetal lothionein-growth hormone fusion genes. Nature 300 611–615.CrossRefGoogle ScholarPubMed
Penman, D.J., Beeching, A.J., Penn, S., and Maclean, N. (1988) Factors affecting survival and integration following microinjection, of novel DNA into rainbow trout eggs. Aquaculture (in the press).Google Scholar
Scott, G.K., Hew, C.L., and Davies, P.L. (1985) Antifreeze protein genes are tandemly linked and clustered in the genome of the winter flounder. Proc. Natl. Acad. Sci. 82 2613–2617.Google Scholar
Sekine, S., Mizukami, T., Nishi, T., Kuana, Y., Saito, A., Sato, M., Itoh, S. and Kawauhi, H. (1985) Cloning and expression of cUNA for salmon growth hormone in Escherichia coli. Proc. Nat. Acad. Sci. 82 4306–4310.Google Scholar
Spradling, A.C. and Rubin, C.M. (1983) The effect of chromosomal position on the expression of the Drosophila xanthine dehydrogenase gene. Cell 34: 47–57.Google Scholar
Stuart, G.W., McMurray, J.V. and Westerfield, (1988) Replication, integration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos. Development 103 403–412.Google Scholar
Thorgaard, G.H. (1986) Ploidy manipulation and performance. Aquaculture 57 57–64.CrossRefGoogle Scholar
Yoon, S.J., Liu, Z., Kapuscinski, A.R., Hackett, P.B., Faras, A., and Guise, K.S. (1988) Gene transfer by microinjection in fish. Aquaculture (in the press).Google Scholar
Zhu, Z., Li, G., He, L., and Chen, S. (1985) Novel gene transfer into the fertilised eggs of gold fish (Carassius auratus) A. angew lchthyol 1 32–34.Google Scholar
Zhu, Z., Xu, K., Li, G., Xie, Y. and He, L. (1986) Biological effects of human growth hormone gene microinjected into the fertilised eggs of loach Misgurnus anguillicaudatus . Kexue Tongbao Academia Sinica 31. 988–990.Google Scholar