Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-05T13:09:37.943Z Has data issue: false hasContentIssue false
  • English
  • Français

Digestibility of rumen undegradable protein from 40 browse species measured by mobile nylon bag and in vitro techniques

Published online by Cambridge University Press:  27 February 2018

R. J. Kaitho ,
N. N. Umunna ,
I. V. Nsahlai ,
S. Tamminga  and
J. van Bruchem
R. J. Kaitho
Affiliation:
International Livestock Research Institute, PO Box 5689, Addis Ababa, Ethiopia Wageningen Institute of Animal Sciences, Marijkeweg 40, PO Box 338, 6700 AH, Wageningen, The Netherlands
N. N. Umunna
Affiliation:
International Livestock Research Institute, PO Box 5689, Addis Ababa, Ethiopia
I. V. Nsahlai
Affiliation:
International Livestock Research Institute, PO Box 5689, Addis Ababa, Ethiopia
S. Tamminga
Affiliation:
Wageningen Institute of Animal Sciences, Marijkeweg 40, PO Box 338, 6700 AH, Wageningen, The Netherlands
J. van Bruchem
Affiliation:
Wageningen Institute of Animal Sciences, Marijkeweg 40, PO Box 338, 6700 AH, Wageningen, The Netherlands
Get access

Extract

Crude protein concentration and energy value of cereal crop residues and poor quality grasses are below animal requirements during much of the year and browses have been incorporated in the feeding systems to improve the nutritional status of ruminants. Leaves and fruits of leguminous browses can be used as protein supplements for ruminants and are commonly used as foods in many agricultural systems. The new systems of protein evaluation (Agricultural and Food Research Council, 1992; Tamminga et al., 1994) partition food nitrogen into the amount degraded in the rumen and that which escapes ruminal degradation. Protein available for absorption post-ruminally is influenced by the amount of food nitrogen that is resistant to ruminal degradation plus microbial protein (Brown and Pitman, 1991). Several studies have been carried out to assess the digestibility of ruminal escape protein (Hvelplund, 1985; Frydrych, 1992), and the digestibility varies considerably across foods (Krishnamoorthy et al., 1982). Values between 0·80 and 0·85 are adopted for the true digestibility of protein from temperate foods and from microbial amino acids (van Bruchem et ah, 1989). However, no data are available on intestinal digestibility of browses although they have been recognized as good protein supplements. The mobile nylon bag (MNB) method is useful for assessing the digestibility of rumen undegradable food protein in the intestine (de Boer et ah, 1987). Since the MNB is labourious, it was desirable to assess the accuracy with which an in vitro (pepsin/pancreatin) procedure could predict results from the former. Therefore, the objective of this study was to determine apparent digestibility of browse rumen undegradable protein using MNB method and pepsin/pancreatin in vitro technique.

Type
Use of enzymes in in vitro methods
Information
BSAP Occasional Publication , Volume 22: In vitro techniques for measuring nutrient supply to ruminants , 1998 , pp. 123 - 126
Copyright
Copyright © British Society of Animal Science 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agricultural and Food Research Council. 1992. Technical Committee on Responses to Nutrients, report no 9. Nutritive requirements of ruminant animals: protein. Nutrition Abstracts and Reviews, Series B 62: 787–835.Google Scholar
Antoniewicz, A., Pisulewski, P. and Szymczyk, B. 1988. The effect of formaldehyde treatment of full-fat rapeseed on pepsin-pancreatin solubility and amino acid composition of rumen undegraded protein. Proceedings of the VI world conference on animal production, 27 June-1 July 1988, Helsinki, p. 306.Google Scholar
Association of Official Analytical Chemists. 1990. Official methods of analysis, 14th edition. Association of Official Analytical Chemists, Washington, DC, USA.Google Scholar
Boer, G. de, , Murphy, J. J. and Kennelly, J. J. 1987. Mobile nylon bag for estimating intestinal availability of rumen undegradable protein, journal of Dairy Science 70: 977–982.Google ScholarPubMed
Brown, W. F. and Pitman, W. D. 1991. Concentration and degradation of nitrogen and fibre fractions in selected grasses and legumes. Tropical Grasslands 25: 305–312.Google Scholar
Buxton, D. R. 1996. Quality-related characteristics of forages as influenced by plant environment and agronomic factors. Animal Feed Science and Technology 59: 37–49.CrossRefGoogle Scholar
Eys, J. E. van, , Mathius, I. W., Pongsapan, P. and Johnson, W. L. 1986. Foliage of the tree legumes Gliricidia, Leucaena and Sesbania as supplement to napier grass diets for growing goats. Journal of Agricultural Science, Cambridge 107: 227–233.Google Scholar
Frydrych, Z. 1992. Intestinal digestibility of rumen undegraded protein of various feeds as estimated by the mobile bag technique. Animal Feed Science and Technology 37: 161–172.CrossRefGoogle Scholar
Hvelplund, T. 1985. Digestibility of rumen microbial protein and undegraded dietary protein estimated in the small intestine of sheep and by in sacco procedure. Acta Agricultural Scandinavica, Supplement 25: 132–144.Google Scholar
Kaitho, R. J., Tamminga, S. and Bruchem, J. van, . 1993. Chemical composition and nylon bag degradation characteristics of dried Calliandra calothyrsus leaves. Animal Feed Science and Technology 43: 23–45.CrossRefGoogle Scholar
Kaitho, R. J., Umunna, N. N., Nsahlai, I. V., Tamminga, S., Bruchem, J. van, , Hanson, J. and Wouw, M. van de, . 1996. Palatability of multipurpose tree species: effect of species and length of study on intake and relative palatability by sheep. Agroforestry Systems 33: 249–261.CrossRefGoogle Scholar
Krishnamoorthy, U., Sniffen, C. J. and Van Soest, P. J. 1982. Nitrogen fractionation in ruminant feedstuffs for feed evaluation. Proceedings of the Cornell nutrition conference, pp. 95–102.Google Scholar
Statistical Analysis Systems Institute. 1987. Procedures guide for personal computers, version 6 edition. Statistical Analysis Systems Institute Inc., Cary, NC, USA.Google Scholar
Tamminga, S., Van Straalen, W. M., Subnel, A. P. J., Meijer, R. G. M., Steg, A., Wever, C. J. G. and Blok, M. C. 1994. The Dutch protein evaluation system: the DVE/OEB-system. Livestock Production Science 40: 139–155.CrossRefGoogle Scholar
Van Bruchem, J. Van, , Bongers, L. J. G. M, Lammers-Wienhoven, S. C. W., Bangma, G. A. and Adrichem, P. W. M. van, . 1989. Apparent and true digestibility of protein and amino acids in the small intestine of sheep as related to the duodenal passage of protein and non-protein dry matter. Livestock Production Science 23: 317–327.CrossRefGoogle Scholar
Van Soest, P. J. and Robertson, J. B. 1985. Analysis of forages and fibrous foods. AS 613 manual, Department of Animal Science, Cornell University, Ithaca, NY.Google Scholar
Straalen, W. M. van, , Dooper, F. M. H., Antoniewicz, A. M., Kosmala, I. and Vuuren, A. M. van, . 1993. Intestinal digestibility in dairy cows of protein from grass and clover measured with mobile nylon bag and other methods. Journal of Dairy Science 76: 2970–2981.CrossRefGoogle ScholarPubMed