Published online by Cambridge University Press: 27 February 2018
Until the early 1970s, selenium (Se) status in livestock had been assessed by the chemical measurement of the element Se. For example, the Watkinson (1966) method requires solubilizing Se by inorganic acids, forming a chemical complex, solvent extraction and fluorimetric detection. By contrast, erythrocyte glutathione peroxidase (GSH-Px), a Se-containing enzyme, is readily assayed in blood haemolysates on automated enzyme analyzers. This enzyme activity is usually related to dietary Se intake during preceding months. For both cattle and sheep, a correlation between erythrocyte GSH-Px and whole blood Se was shown (Anderson, Berrett and Patterson, 1978).