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6.2 Selenium Deficiency in Farm Livestock in Britain

Published online by Cambridge University Press:  27 February 2018

P. H. Anderson*
Affiliation:
MAFF Central Veterinary Laboratory, New Haw, Weybridge, Surrey, KT15 3NB
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Extract

Until the early 1970s, selenium (Se) status in livestock had been assessed by the chemical measurement of the element Se. For example, the Watkinson (1966) method requires solubilizing Se by inorganic acids, forming a chemical complex, solvent extraction and fluorimetric detection. By contrast, erythrocyte glutathione peroxidase (GSH-Px), a Se-containing enzyme, is readily assayed in blood haemolysates on automated enzyme analyzers. This enzyme activity is usually related to dietary Se intake during preceding months. For both cattle and sheep, a correlation between erythrocyte GSH-Px and whole blood Se was shown (Anderson, Berrett and Patterson, 1978).

Type
6. Poster Presentations
Copyright
Copyright © British Society of Animal Production 1983

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References

Anderson, P. H., Berrett, S. and Patterson, D. S. P. 1978. Glutathione peroxidase activity in erythrocytes and muscle of cattle and sheep and its relationship with selenium. J. Comp. Path. 88: 181189.CrossRefGoogle ScholarPubMed
Anderson, P. H., Berrett, S. and Patterson, D. S. P. 1979. The biological selenium status of livestock in Britain indicated by sheep erythrocyte glutathione peroxidase activity. Vet. Rec. 104: 235238.Google Scholar
Watkinson, J. H. 1966. Fluorimetrie determination of selenium in biological material with 2, 3-Diaminonaphthalene. Anal. Chem. 38: 9297.Google Scholar