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Wine constituents inhibit thrombosis but not atherogenesis in C57BL/6apolipoprotein E-deficient mice

Published online by Cambridge University Press:  08 March 2007

Thierry Soulat ,
Catherine Philippe ,
Claire Bal dit Sollier ,
Christophe Brézillon ,
Natacha Berge ,
Pierre-Louis Teissedre ,
Jacques Callebert ,
Sylvie Rabot  and
Ludovic Drouet
Thierry Soulat
Affiliation:
Laboratoire de Thrombose et d'Athérosclérose Expérimentales, IVS, Hôpital Lariboisière, 2 rue Ambroise Paré, 75475 Paris Cedex 10, France
Catherine Philippe
Affiliation:
Unité d'Ecologie et Physiologie du Système Digestif, INRA, 78352 Jouy-en-Josas Cedex, France
Claire Bal dit Sollier
Affiliation:
Laboratoire de Thrombose et d'Athérosclérose Expérimentales, IVS, Hôpital Lariboisière, 2 rue Ambroise Paré, 75475 Paris Cedex 10, France
Christophe Brézillon
Affiliation:
Unité d'Ecologie et Physiologie du Système Digestif, INRA, 78352 Jouy-en-Josas Cedex, France
Natacha Berge
Affiliation:
Laboratoire de Thrombose et d'Athérosclérose Expérimentales, IVS, Hôpital Lariboisière, 2 rue Ambroise Paré, 75475 Paris Cedex 10, France
Pierre-Louis Teissedre
Affiliation:
Faculté d'Œnologie, Université Victor Segalen Bordeaux 2, 351 cours de la Libération, 33405 Talence Cedex, France
Jacques Callebert
Affiliation:
Laboratoire de Biochimie, Hôpital Lariboisière, 2 rue Ambroise Parè, 75475 Paris Cedex 10, France
Sylvie Rabot
Affiliation:
Unité d'Ecologie et Physiologie du Système Digestif, INRA, 78352 Jouy-en-Josas Cedex, France
Ludovic Drouet*
Affiliation:
Laboratoire de Thrombose et d'Athérosclérose Expérimentales, IVS, Hôpital Lariboisière, 2 rue Ambroise Paré, 75475 Paris Cedex 10, France
*
*Corresponding author: Professor Ludovic Drouet, fax +33 1 49 95 63 97, email [email protected]
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Abstract

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Regular and moderate wine consumption is one of the explanations suggested for the lower incidence of cardiovascular events in France compared with other industrialized countries. We evaluated whether alcohol alone or combined with red wine polyphenols reduced plaque size and/or attenuated thrombotic reactivity at the site of advanced atherosclerotic lesions. Red wine extract, or purified (+)-catechin with alcohol, or alcohol alone, was added for 12 weeks to the drinking water of apoE-deficient (apoE−/−) C57B/ mice and wild-type counterparts. In the apoE−/− mice, all alcohol-containing mixtures were associated with a larger size of aortic atherosclerotic lesions. On the other hand, red wine extract and (+)-catechin significantly inhibited blood thrombotic reactivity (P<0·05) as assessed in a cylindrical perfusion chamber model of experimental thrombosis: area reductions in cross-sectional surface of the ex vivo thrombus were 64% and 63%, respectively. In the wild-type mice, red wine extract and (+)-catechin tended to reduce thrombogenicity, which was on the whole less marked than in the apoE−/− mice. These findings suggest that a moderate and regular consumption of red wine may protect against clinical cardiovascular events, mainly by attenuating the thrombogenic response rather than by reducing the development of atherosclerotic lesions. This antithrombogenic effect may include normalization of the abnormally high thrombogenic responsiveness in apoE−/− mice as well as a direct antithrombotic effect.

Keywords

AtherosclerosisThrombosisPolyphenolsAlcoholApolipoprotein E-deficient mouse
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 2 , August 2006 , pp. 290 - 298
Copyright
Copyright © The Nutrition Society 2006

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