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Whole-body valine and cysteine kinetics and tissue fractional protein synthesis rates in lambs fed Sulla (Hedysarum coronarium) and infected or not infected with adult Trichostrongylus colubriformis

Published online by Cambridge University Press:  08 March 2007

Emma N. Bermingham ,
Warren C. McNabb ,
Ian A. Sutherland ,
Bruce R. Sinclair ,
Bryan P. Treloar  and
Nicole C. Roy
Emma N. Bermingham
Affiliation:
Nutrition and Metabolism Section, Food and Health Group, AgResearch Grasslands, Palmerston North, New Zealand
Warren C. McNabb*
Affiliation:
Nutrition and Metabolism Section, Food and Health Group, AgResearch Grasslands, Palmerston North, New Zealand
Ian A. Sutherland
Affiliation:
Animal Health Group, AgResearch Grasslands, Palmerston North, New Zealand
Bruce R. Sinclair
Affiliation:
Nutrition and Metabolism Section, Food and Health Group, AgResearch Grasslands, Palmerston North, New Zealand
Bryan P. Treloar
Affiliation:
Nutrition and Metabolism Section, Food and Health Group, AgResearch Grasslands, Palmerston North, New Zealand
Nicole C. Roy*
Affiliation:
Nutrition and Metabolism Section, Food and Health Group, AgResearch Grasslands, Palmerston North, New Zealand
*
*Corresponding authors: Dr Nicole Roy and Dr Warren McNabb, fax + 64 6 351 8003, email [email protected], email [email protected]
*Corresponding authors: Dr Nicole Roy and Dr Warren McNabb, fax + 64 6 351 8003, email [email protected], email [email protected]
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Abstract

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Poor growth during parasitic infection may be due to a redistribution of amino acids away from skeletal muscle protein synthesis to the intestinal site of infection. The effect of a Trichostrongylus colubriformis infection on whole-body amino acid kinetics and tissue fractional protein synthesis rates were determined in lambs fed fresh Sulla (Hedysarum coronarium; 800g DM/d). Lambs were dosed with 6000 L3 Trichostrongylus colubriformis larvae daily for 6d (n 6) or kept as parasite-free controls (n 6). On day 45 post-infection, the lambs received an intravenous injection of 2H2O and infusions (8h) of [35S]sulphate to measure the size of the whole-body water and sulphate pools, respectively. On day 48, the lambs were continuously infused for 8h with [3,4-3H]valine into the jugular vein as well as with [1-13C]valine and [35S]cysteine into the abomasum. After the 8h infusions, the lambs were killed and tissue samples collected from the duodenum, ileum, mesenteric lymph nodes, liver, spleen, thymus, muscle and skin. Feed intake (769 v. 689 (sd 47) g DM/d) was not affected by infection, whereas liveweight gains (50 v. −50 (sd 70) g/d) were lower and intestinal worm burdens(240 v. 18 000 (sd 7000) worms) higher in the infected lambs. Parasitic infection increased the fractional protein synthesis rates in the small intestine, mesenteric lymph nodes and liver but did not affect skin and skeletal muscle fractional protein synthesis rates during the established parasitic infection.

Keywords

Parasite infectionWhole-body amino acid kineticsTissue protein synthesisSheep
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 1 , January 2006 , pp. 28 - 38
Copyright
Copyright © The Nutrition Society 2006

References

Baracos, VE, Brun-Bellut, J, Marie, M, Tissue protein synthesis in lactating and dry goats. Br J Nutr (1991) 66 451–465.CrossRefGoogle ScholarPubMed
Beckett, PR, Cadenhead, A, Fuller, MF, Valine oxidation: the synthesis and evaluation of L-[-3H]-valine as a tracer in vivo. Br J Nutr (1992) 68 139–151.CrossRefGoogle ScholarPubMed
Bermingham, ENThe metabolic cost of an intestinal parasite infection on amino acid kinetics in sheep fed fresh forages. PhD Thesis, New Zealand. Massey University, 2004.Google Scholar
Bermingham, EN, Hutchinson, KJ, Revell, DK, Brookes, IM, McNabb, WC, The effect of condensed tannins in Sainfoin (Onobrychi Viciifolia) and Sulla (Hedysarum coronarium) on the digestion of amino acids in sheep. Proc NZ Soc Anim Prod (2001) 61 116–119.Google Scholar
Bidlingmeyer, BA, Cohen, SA, Tarvin, TL, Rapid analysis of amino acids using pre-column derivatisation. J Chromatogr (1984) 336 93–104.CrossRefGoogle Scholar
Breuillé, D, Rose, F, Arnal, M, Meilin, C, Obled, C, Sepsis modifies the contribution of different organs to whole-body protein synthesis in rats. Clin Sci (1994) 86 663–669.CrossRefGoogle ScholarPubMed
Burke, JL, Waghorn, GC, Brookes, IM, An evaluation of sulla (Hedysarum coronarium) with pasture, white clover and Lucerne for lambs. Proc NZ Soc Anim Prod (2002) 62 152–156.Google Scholar
Connell, A, Calder, AG, Anderson, SE, Lobley, GE, Hepatic protein synthesis in the sheep: effect of intake as monitored by use of stable-isotope-labelled glycine, leucine and phenylalanine. Br J Nutr (1997) 77 255–271.Google Scholar
Coop, RL, Angus, KW, The effect of continuous doses of Trichostrongylus colubriformis larvae on the intestinal mucosa of sheep and on liver vitamin A concentration. Parasitology (1975) 70 1–9.CrossRefGoogle ScholarPubMed
Davis, SR, Barry, TN, Hughson, GA, Protein synthesis in tissues of growing lambs. Br J Nutr (1981) 46 409–419.CrossRefGoogle ScholarPubMed
Davis, TA, Fiorotto, ML, Burrin, DG, Vann, RCProtein synthesis in organs and tissues: quantitative methods in laboratory animals InMethods for Investigation of Amino Acid and Protein Metabolism pp. (El-Khoury, A) New York: CRC Press (1999) 49–68.Google Scholar
Gaitonde, MK, A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids. Biochem J (1967) 104 627–633.CrossRefGoogle ScholarPubMed
Harris, PM, Skene, PA, Buchan, V, Milne, E, Calder, AG, Anderson, SE, Connell, A, Lobley, GE, Effect of food intake on hind-limb and whole-body protein metabolism in young growing sheep: chronic studies based on arterio-venous techniques. Br J Nutr (1992) 68 389–407.CrossRefGoogle ScholarPubMed
Huntington, GB, Reynolds, CK, Stroud, BH, Techniques for measuring blood flow in splanchnic tissues of cattle. J Dairy Sci (1989) 72 1583–1595.CrossRefGoogle ScholarPubMed
Jones, WO, Symons, LEA, Protein synthesis in the whole body, liver, skeletal muscle and kidney cortex of lambs infected withTrichostrongylus colubriformis. Int J Parasitol (1982) 12 295–301.CrossRefGoogle ScholarPubMed
Krempf, M, Hoerr, RA, Marks, L, Young, VR, Phenylalanine flux in adult men: estimates with different tracers and route of administration. Metabolism (1990) 39 560–562.CrossRefGoogle ScholarPubMed
Lee, J, Harris, PM, Sinclair, BR, Treloar, BP, Whole body metabolism of cysteine and glutathione and their utilisation in the skin of Romney sheep: consequences of wool growth. J Agric Sci (Camb) (1993) 121 111–124.CrossRefGoogle Scholar
Lee, J, Knutson, RJ, Davis, SR, Louie, K, Mackenzie, DDS, Harris, PM, Sulphur amino acid metabolism in the whole body and mammary gland of the lactating Saanen goat. Aust J Agric Res (1999) 50 413–423.CrossRefGoogle Scholar
Lobley, GE, Connell, A, Lomax, MA, Brown, DS, Milne, E, Calder, AG, Farningham, DAH, Hepatic detoxification of ammonia in the ovine liver: possible consequences for amino acid catabolism. Br J Nutr (1995) 73 667–685.CrossRefGoogle ScholarPubMed
Lobley, GE, Connell, A, Milne, E, Newman, A, Ewing, TA, Protein synthesis in splanchnic tissues of sheep offered two levels of intake. Br J Nutr (1994) 71 3–12.CrossRefGoogle ScholarPubMed
Lobley, GE, Connell, A, Revell, DK, Bequette, BJ, Brown, DS, Calder, AG, Splanchnic-bed transfers of amino acids in sheep blood and plasma, as monitored through use of a multiple U-13C-labelled amino acid mixture. Br J Nutr (1996) 75 217–235.CrossRefGoogle ScholarPubMed
MacRae, jc, Metabolic consequences of intestinal parasitism. Proc Nutr Soc (1993) 52 121–130.CrossRefGoogle ScholarPubMed
MacRae, jc, Lobley, ge, Physiological and metabolic implications of conventional and novel methods for the manipulation of growth and production. Livest Prod Sci (1991) 27 43–59.Google Scholar
MacRae, JC, Walker, A, Brown, D, Lobley, GE, Accretion of total protein and individual amino acids by organs and tissues of growing lambs and the ability of nitrogen balance techniques to quantitate protein retention. Anim Prod (1993) 57 237–245.Google Scholar
McNabb, WC, Waghorn, GC, Barry, TN, Shelton, ID, The effect of condensed tannins in Lotus pedunculatus on the digestion and metabolism of methionine, cystine and inorganic sulphur in sheep. Br J Nutr (1993) 70 647–661.CrossRefGoogle ScholarPubMed
Ortigues, I, Durand, DAdaptation of energy metabolism to undernutrition in ewes. Contribution of portal-drained viscera, liver and hindquarters. Br J Nutr (1995) 73 209–226.CrossRefGoogle ScholarPubMed
Sutherland, IA, Brown, AE, Green, RS, Miller, CM, Leathwick, DM, The immune response of sheep to larval challenge withOstertagia circumcinta and O. ostertagi. Vet Parasitol (1999a) 84 125–135.CrossRefGoogle Scholar
Sutherland, IA, Leathwick, DM, Green, R, Brown, AE, Miller, CM, The effect of continuous drug exposure on the immune response to Trichostrongylus colubriformis in sheep. Vet Parasitol (1999b) 80 261–271.CrossRefGoogle ScholarPubMed
Sykes, AR, Coop, RL, Intake and utilisation of food by growing lambs with parasitic damage to the small intestine caused by daily dosing with Trichostrongylus colubriformis larvae. J Agric Sci (Camb) (1976) 86 507–515.CrossRefGoogle Scholar
Sykes, AR, Poppi, DP, Elliot, DC, Effect of concurrent infection with Ostertagia circumcincta and Trichostrongylus colubriformis on the performance of growing lambs consuming fresh forages. J Agric Sci (Camb) (1988) 110 531–541.CrossRefGoogle Scholar
Symons, LEAEpithelial cell mitosis and morphology in wormfree regions of the intestine of the rat infected byNippostrongylus brasiliensis. J Parasitol (1978) 64 958–959.Google Scholar
Symons, LEA, Jones, WO, Protein metabolism 1. Incorporation of 14C-L-leucine into skeletal muscle and liver proteins of mice and guinea pigs infected withNematospiroides dubis and Trichostrongylus colubriformis. Exp Parasitol (1971) 29 230–241.Google Scholar
Symons, LEA, Jones, WO, Protein metabolism 2. Protein turnover, synthesis and muscle growth in suckling, young and adult mammals infected withNematospiriodes dubuis or Trichostrongylus colubriformis. Exp Parasitol (1972) 32 335–342.CrossRefGoogle ScholarPubMed
Symons, LEA, Jones, WO, Skeletal muscle, liver and wool protein synthesis by sheep infected by the nematodeTrichostrongylus colubriformis. Aust J Agric Res (1975) 26 1063–1072.Google Scholar
Symons, LEA, Jones, WO, Protein metabolism 5. Trichostrongylus colubriformis: changes of host body mass and protein synthesis in guinea pigs with light to heavy infections. Exp Parasitol (1978) 44 7–13.CrossRefGoogle ScholarPubMed
Symons, LEA, Jones, WO, Intestinal protein synthesis in guinea pigs infected withTrichostrongylus colubriformis. Int J Parasito (1983) 13 309–312.CrossRefGoogle ScholarPubMed
van Houtert, MFJ, Barger, IA, Steel, JW, Windon, RG, Emery, DL, Effects of dietary protein intake on responses of young sheep to infection withTrichostrongylus colubriformis. Vet Parasitol (1995) 56 163–180.CrossRefGoogle ScholarPubMed
Vlassoff, A, McKenna, PB, Nematode parasites of economic importance in sheep in New Zealand. NZ J Zool (1994) 21 1–8.Google Scholar
Wang, Y, Waghorn, GC, Barry, TN, Shelton, ID, The effect of condensed tannins in Lotus corniculatus on plasma metabolism of methionine, cystine and inorganic sulphate by sheep. Br J Nutr (1994) 72 923–935.CrossRefGoogle ScholarPubMed
Waterlow, JC, Garlick, PJ, Millward, DJ (1978) Protein Turnover in Mammalian Tissues and in the Whole Body Amsterdam: North-Holland Biomedical Press.Google Scholar
Whitlock, H, Some modifications of the McMaster helminth egg-counting technique and apparatus. J Council Sci Indusl Res Austr (1948) 21 177–180.Google Scholar
Wykes, LJ, Fiorotto, M, Burrin, DG, Del Rosario, M, Frazer, ME, Pond, WG, Jahoor, F, Chronic low protein intake reduces tissue protein synthesis in a pig model of protein malnutrition. J Nutr (1996) 126 1481–1488.CrossRefGoogle Scholar
Yang, D, Diraison, F, Beylot, M, Brunengraber, DZ, Samols, MA, Anderson, VE, Brunengraber, H, Assay of low deuterium enrichment of water by isotopic exchange with [U-13C3]acetone and gas chromatography-mass spectrometry. Anal Biochem (1998) 258 315–321.Google Scholar
Yu, F, Bruce, LA, Calder, AG, Milne, E, Coop, RL, Jackson, F, Horgan, GW, MacRae, JC, Subclinical infection with the nematode Trichostrongylus colubriformis increases gastrointestinal tract leucine metabolism and reduces availability of leucine for other tissues. J Anim Sci (2000) 78 380–390.CrossRefGoogle ScholarPubMed
Yu, YM, Wagner, DA, Tredget, EE, Walaszewski, JA, Burke, JA, Young, VR, Quantitative role of splanchnic region in leucine metabolism: L-[1-13C,15N]leucine and substrate balance studies. Am J Physiol (1990) 259 E36–E51.Google Scholar