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Vitamin-A-dependent zinc-binding protein and intestinal absorption of Zn in chicks

Published online by Cambridge University Press:  09 March 2007

Nadezhda I. Berzin  and
Victoria K. Bauman
Nadezhda I. Berzin
Affiliation:
Institute of Biology, Salaspils, Latvian SSR, 229021, USSR
Victoria K. Bauman
Affiliation:
Institute of Biology, Salaspils, Latvian SSR, 229021, USSR
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Abstract

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1. It was demonstrated that the development of experimental avitaminosis A in chicks led to secondary zinc deficiency. The balance of Zn in the chick became negative, while the Zn content of various tissues decreased. Thus in vitamin-A-deficient chicks the serum Zn content was 1258 (SD 26.3) μg/l which was considerably lower than 1652 (SD 97.8) μg/l in controls.

2. Zn absorption was considerably reduced throughout the entire small intestine of vitamin-A-deficient chicks and most markedly in the ileal region. Within 72 h after retinyl acetate administration Zn absorption was fully restored in this region of the intestine.

3. The 65Zn-binding capacity of soluble proteins, present in the supernatant fraction of ileal-mucosa homogenates of vitamin-A-deficient chicks, was found to increase 2.6 times by 72 h after the administration of a single dose of retinyl acetate.

4. A vitamin-A-dependent Zn-binding protein (ZnBP), absent in vitamin-A-deficient chicks, was isolated from the ileal mucosa after their repletion with vitamin A. Competitive-binding studies (calcium, cadmium, copper) showed the protein to be highly specific for Zn ions.

5. The molecular weight of ZnBP wa5 83 kDa. The association constant of the protein-Zn complex was 0.8 x 106/mol. The protein was acidic with approximately 20% of its amino acid residues belonging to dicarboxylic acids. ZnBP was found to be a glycoprotein, and it contained hexose as a carbohydrate component.

6. It is suggested that ZnBP is involved in the binding of Zn in the ileal mucosa of chicks.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 57 , Issue 2 , March 1987 , pp. 255 - 268
Copyright
Copyright © The Nutrition Society 1987

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