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Vitamin B6 deficiency experimentally-induced bone and joint disorder: microscopic, radiographic and biochemical evidence

Published online by Cambridge University Press:  10 October 2007

Priscille G. Massé ,
Kenneth P. H. Pritzker ,
Maria G. Mendes ,
Adele L. Boskey  and
Harald Weiser
Priscille G. Massé
Affiliation:
École de nutrition et d'etudes familiales, Université de Moncton, Moncton, New Brunswick, Canada, El A 3E9
Kenneth P. H. Pritzker
Affiliation:
The Connective Tissue Research Group, Department of Pathology, Mount Sinai Hospital. University of Toronto, Toronto, Canada, M5G 1X5
Maria G. Mendes
Affiliation:
The Connective Tissue Research Group, Department of Pathology, Mount Sinai Hospital. University of Toronto, Toronto, Canada, M5G 1X5
Adele L. Boskey
Affiliation:
The Laboratory of Ultrastructural Biochemistry, The Hospital for Special Surgery, New York, 10021, USA
Harald Weiser
Affiliation:
Department of Vitamin Research and Development, Hoffmann–La Roche Ltd., Basel, Switzerland
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Abstract

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In the present study the effect of pyridoxine deficiency on the utrastructure and morphology of bone and its metabolism was examined in the rapidly growing chick. Pyridoxine-deficient animals had tibias of reduced dry weight and cortical thickness. Histomorphometry demonstrated a disproportionately high eroded surface, lower amount of osteoid tissue and reduced mineralized trabecular width. Anterior–posterior radiographs of the tibiotarsometatarsal joint showed reduced secondary ossification centres and coarse trabeculation. Decalcified metaphyseal cartilage showed irregular trabeculas and a markedly reduced amount of Fast-green counterstain matrix suggesting that there is less collagen present and in turn less availability for matrix to be laid down for later calcification. Plasma activity of the bone alkaline phosphatase isoenzyme (EC) 3. 1.3.1) was decreased. Plasma Ca and PO4 levels did not vary. The present bone study referring to a pseudo-lathyritic state in which collagen maturation is not completely achieved supports the hypothesis that pyridoxine is an essential nutrient for the connective tissue matrix.

Type
Effect of vitamin D6 difficiency on bone
Information
British Journal of Nutrition , Volume 71 , Issue 6 , June 1994 , pp. 919 - 932
Copyright
Copyright © The Nutrition Society 1994

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