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Vernon Young and the development of current knowledge in protein and amino acid nutritionVernon Young 1937–2004

Published online by Cambridge University Press:  09 March 2007

D. Joe Millward
Affiliation:
Centre for Nutrition and Food SafetySchool of Biomedical and Molecular SciencesUniversity of SurreyGuildfordSurrey GU2 [email protected]
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Abstract

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Type
Appreciation
Copyright
Copyright © The Nutrition Society 2004

References

Alexis, SD, Young, VR & Gill, DMConcentration of elongation factor 2 in rat skeletal muscle during protein depletion and re-feeding. Biochem J (1974) 142, 185188.CrossRefGoogle ScholarPubMed
Bolourchi, S, Friedmann, CM & Mickelsen, OWheat flour as a source of protein for human subjects. Am J Clin Nutr (1968) 21, 827835.CrossRefGoogle ScholarPubMed
Calloway, DH & Margen, SVariation in endogenous nitrogen excretion and dietary nitrogen utilization as determinants of human protein requirements. J Nutr (1971) 101, 205216.CrossRefGoogle Scholar
Carpenter, KJA short history of nutritional science: part 1(1785–1885). J Nutr (2003 a) 133, 638645.CrossRefGoogle Scholar
Carpenter, KJA short history of nutritional science: part 2 (1885–1912). J Nutr (2003 b) 133, 975984.CrossRefGoogle Scholar
Carpenter, KJA short history of nutritional science: part 3 (1912–1944). J Nutr (2003 c) 133, 30233032.CrossRefGoogle Scholar
Carpenter, KJA short history of nutritional science: part 4 (1945–1985). J Nutr (2003 d) 133, 33313342.CrossRefGoogle Scholar
Chen, SC & Young, VRPreparation and some properties of rat skeletal muscle polyribosomes. Biochem J (1968) 106, 6167.CrossRefGoogle ScholarPubMed
El-Khoury, AE, Ajami, AM, Fukagawa, NK, Chapman, TE & Young, VRDiurnal pattern of the interrelationships between leucine oxidation, urea production and hydrolysis in humans. Am J Physiol (1996) 271, E563E573.Google ScholarPubMed
El-Khoury, AE, Fukagawa, NK, Sánchez, M, Tsay, RH, Gleason, RE, Chapman, TE & Young, VRThe 24 hour pattern and rate of leucine oxidation, with particular reference to tracer estimates of leucine requirements in healthy adults. Am J Clin Nutr (1994) 59, 10121020.CrossRefGoogle ScholarPubMed
Food and Agriculture Organization Protein Requirements:Report of the FAO Committee. FAO Nutritional Studies. no.16 Rome. FAO. (1957).Google Scholar
Food and Agriculture OrganizationWorld Health Organization Protein Requirements: Report of a Joint FAO/WHO Expert Group.WHO Technical Report Series no. 301 Geneva: WHO. (1965).Google Scholar
Food and Agriculture Organization/World Health Organization Energy and Protein Requirements. Report of a Joint FAO/WHO Ad Hoc Expert Committee. Technical Report Series. no. 522 Geneva: WHO. (1973).Google Scholar
Food and Agriculture Organization/World Health Organization/United Nations University Energy and Protein Requirements. Report of a Joint FAO/WHO/UNU Expert Consultation. Technical Report Series no. 724 Geneva: WHO. (1985).Google Scholar
Furst, P15 N-studies in severe renal failure. II. Evidence for the essentiality of histidine. Scand J Clin Lab Invest (1972) 30, 307312.CrossRefGoogle ScholarPubMed
Garza, C, Scrimshaw, NS & Young, VRHuman protein requirements: a long-term metabolic nitrogen balance study in young men to evaluate the 1973 FAO/WHO safe level of egg protein intake. J Nutr (1977 a) 107, 335352.CrossRefGoogle Scholar
Garza, C, Scrimshaw, NS & Young, VRHuman protein requirements: evaluation of the 1973 FAO/WHO safe level of protein intake for young men at high energy intakes. Br J Nutr (1977 b) 37, 403420.CrossRefGoogle ScholarPubMed
Garza, C, Scrimshaw, NS & Young, VRHuman protein requirements: interrelationships between energy intake and nitrogen balance in young men consuming the 1973 FAO/WHO safe level of egg protein, with added nonessential amino acids. J Nutr (1978) 108, 9096.CrossRefGoogle Scholar
Gersovitz, M, Motil, KJ,Munro, HN, Scrimshaw, NS & Young, VRHuman protein requirements: assessment of the adequacy of the current recommended dietary allowance for dietary protein in elderly men and women. Am J Clin Nutr (1982) 35, 614.CrossRefGoogle ScholarPubMed
Giordano, C, de Pascale, C, Baliestrieri, C, Cittadini, D & Crescenzi, AIncorporation of urea 15 N in amino acids of patients with chronic renal failure on low nitrogen diet. Am J Clin Nutr (1968) 21, 394404.CrossRefGoogle Scholar
Hegsted, DMVariation in requirements of nutrients - amino acids. Fed Proc (1963) 22, 14241430.Google ScholarPubMed
Hegsted, DMFrom chick nutrition to nutrition policy. Annu Rev Nutr (2000) 20, 119.CrossRefGoogle ScholarPubMed
Institute of Medicine Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol, Protein, and Amino Acids (Macronutrients) Washington, DC: National Academy Press. (2002).Google Scholar
Irwin, MI & Hegsted, DMA conspectus of research on amino acid requirements of man. J Nutr (1971) 101, 539566.CrossRefGoogle ScholarPubMed
Istfan, N, Murray, E, Janghorbani, M, Evans, WJ & Young, VYNutritional value of a soy protein concentrate (STAPRO-3200) for long-term protein nutritional maintenance in young men. J Nutr (1983 a) 113, 25242534.CrossRefGoogle ScholarPubMed
Istfan, N, Murray, E, Janghorbani, M & Young, VRAn evaluation of a soy protein concentrate in young men using the short-term N balance method. J Nutr (1983 b) 113, 25162523.CrossRefGoogle ScholarPubMed
Jackson, AASalvage of urea-nitrogen in the large bowel: functional significance in metabolic control and adaptation. Biochem Soc Trans (1998) 26, 231236.CrossRefGoogle ScholarPubMed
Jackson, AALimits of adaptation to high dietary protein intakes. Eur J Clin Nutr (1999) 53, Suppl. 1 S44S52.CrossRefGoogle ScholarPubMed
Jackson, AAMechanisms of adaptation: the constancy of urea production. In Nutritional Metabolism and Malnutrition: A Festschrift for John Conrad Waterlow, 7179 [Shetty, P editors]. London. Smith-Gordon. (2000).Google Scholar
James, WPT & Hay, AMAlbumin metabolism: effect of the nutritional state and the dietary protein intake. J Clin Invest (1968) 47, 19581972.CrossRefGoogle ScholarPubMed
Kien, CL, Rohrbaugh, DK, Burke, JF & Young, VRWhole body protein synthesis in relation to basal energy expenditure in healthy children and in children recovering from burn injury. Pediatr Res (1978 a) 12, 211216.CrossRefGoogle ScholarPubMed
Kien, CL, Young, VR, Rohrbaugh, DK & Burke, JFWhole body protein synthesis and breakdown rates in children before and after reconstructive surgery of the skin. Metabolism (1978 b) 27, 2734.CrossRefGoogle ScholarPubMed
Kien, CL, Young, VR, Rohrbaugh, DK & Burke, JFIncreased rates of whole body protein synthesis and breakdown in children recovering from burns. Ann Surg (1978 c) 187, 383391.CrossRefGoogle ScholarPubMed
Kurpad, AV, Raj, T, El-Khoury, A, Beaumier, L, Kuriyan, R, Srivatsa, A, Borgonha, S, Selvaraj, A, Regan, MM & Young, VRLysine requirement of healthy adult Indian subjects, measured by an indicator amino acid balance technique. Am J Clin Nutr (2001 a) 73, 900907.CrossRefGoogle ScholarPubMed
Kurpad, AV, Raj, T, El-Khoury, A, Kuriyan, R,Maruthy, K, Borgonha, S, Chandukudlu, D, Regan, MM & Young, VRDaily requirement for and splanchnic uptake of leucine in healthy adult Indians. Am J Clin Nutr (2001 b) 74, 747755.CrossRefGoogle ScholarPubMed
Kurpad, AV, Raj, T, Regan, MM, Vasudevan, J, Caszo, B, Nazareth, D, Gnanou, J & Young, VRThreonine requirements of healthy Indian adults, measured by a 24 h indicator amino acid oxidation and balance technique. Am J Clin Nutr (2002 a) 76, 789797.CrossRefGoogle ScholarPubMed
Kurpad, AV, Regan, MM, Nazareth, D, Nagaraj, S, Gnanou, J & Young, VRIntestinal parasites increase dietary lysine requirement in chronically undernourished adult Indian men. Am J Clin Nutr (2003 a) 78, 11451151.CrossRefGoogle Scholar
Kurpad, AV, Regan, MM, Raj, T, El-Khoury, A, Kuriyan, R, Vaz, M, Chandakudlu, D, Venkataswamy, VG, Borgonha, S & Young, VRLysine requirements of healthy adult Indian subjects receiving long-term feeding measured with a 24-h indicator amino acid oxidation and balance technique. Am J Clin Nutr (2002 b) 76, 404412.CrossRefGoogle ScholarPubMed
Kurpad, AV, Regan, MM, Raj, T, Varalakshmi, S, Gnanou, J, Thankachan, P & Young, VRLeucine requirement and splanchnic uptake of leucine in chronically undernourished adult Indian subjects. Am J Clin Nutr (2003 b) 77, 861867.CrossRefGoogle ScholarPubMed
Kurpad, AV, Regan, MM, Raj, T, Vasudevan, J, Kuriyan, R, Gnanou, J & Young, VRLysine requirement of chronically undernourished adult Indian men, measured by the 24 h indicator amino acid oxidation and balance technique. Am J Clin Nutr (2003 c) 77, 101108.CrossRefGoogle ScholarPubMed
Kurpad, AV, Regan, MM, Varalakshmi, S, Gnanou, J, Lingappa, A & Young, VREffect of cystine on the methionine requirement of healthy adult Indian subjects, determined using the 24 h indicator amino acid balance approach. Am J Clin Nutr, (2004) (In the Press).CrossRefGoogle Scholar
Kurpad, AV, Regan, MM, Varalakshmi, S, Vasudevan, J, Gnanou, J, Raj, T & Young, VRThe daily methionine requirements of healthy Indian men, measured by a 24 h indicator amino acid oxidation and balance technique. Am J Clin Nutr (2003 d) 77, 11981205.CrossRefGoogle Scholar
McLaren, DSThe great protein fiasco. Lancet (1974) ii, 9396.CrossRefGoogle Scholar
Marchini, JS, Cortiella, J, Hiramatsu, T, Chapman, TE & Young, VRRequirements for indispensable amino acids in humans: longer-term amino acid study with support for the adequacy of the MIT amino acid requirement pattern. Am J Clin Nutr (1993) 58, 670683.CrossRefGoogle ScholarPubMed
Matthews, DE, Bier, DM, Rennie, MJ, Edwards, RHT, Halliday, D, Millward, DJ & Clugston, GRegulation of leucinemetabolism in man: a stable isotope study. Science (1981) 214, 11291131.CrossRefGoogle Scholar
Meguid, MM, Matthews, DE, Bier, DM, Meredith, CN, Soeldner, JS, Young, VRLeucine kinetics at graded leucine intakes in young men. Am J Clin Nutr (1986 a) 43, 770780.CrossRefGoogle ScholarPubMed
Meguid, MM, Matthews, DE, Bier, DM, Meredith, CN & Young, VRValine kinetics at graded valine intakes in young men. Am J Clin Nutr (1986 b) 43, 781786.CrossRefGoogle ScholarPubMed
Meredith, C, Bier, DM, Meguid, MM, Matthews, DE, Wen, Z & Young, VRWhole body amino acid turnover with 13C tracers: a new approach for estimation of human amino acid requirements. In Clinical Nutrition 81, pp.4259 [RIC, Wesdorp and PB, Soeters, editors]. London and New York: Churchill Livingstone. (1982)Google Scholar
Meredith, CN, Wen, Z-M, Bier, DM, Mathews, DE & Young, VRLysine kinetics at graded lysine intakes in young men. Am J Clin Nutr (1986) 43, 787794.CrossRefGoogle ScholarPubMed
Metges, CC, El-Khoury, AE, Henneman, L, Petzke, KJ, Grant, I, Bedri, S, Pereira, PP, Ajami, AM, Fuller, MF & Young, VRAvailability of intestinal microbial lysine for whole body lysine homeostasis in human subjects. Am J Physiol (1999 a) 277, E597E607.Google ScholarPubMed
Metges, CC, Petzke, KJ, El-Khoury, AE, Henneman, L, Grant, I, Bedri, S, Fuller, MF & Young, VRIncorporation of urea and ammonia nitrogen into ileal and fecal microbial proteins and plasma free amino acids in normal men and ileostomates. Am J Clin Nutr (1999 b) 70, 10461058.CrossRefGoogle ScholarPubMed
Millward, DJAmino acid requirements in adult man. Am J Clin Nutr (1990) 51, 492493.CrossRefGoogle ScholarPubMed
Millward, DJCan we define indispensable amino acid requirements and assess protein quality in adults?. J Nutr (1994) 124, 1509S1516S.CrossRefGoogle ScholarPubMed
Millward, DJAn adaptive metabolic demand model for protein and amino acid requirements. Br J Nutr (2003) 90, 249260.CrossRefGoogle ScholarPubMed
Millward, DJ & Bates, PC3-Methylhistidine turnover in the whole body: the contribution of skeletal muscle and intestine to urinary 3-methylhistidine excretion in the adult rat. Biochem J (1983) 214, 603615.CrossRefGoogle ScholarPubMed
Millward, DJ, Bates, PC, Grimble, GK, Brown, JC, Natham, M & Rennie, MJThe contribution of non-skeletal muscle tissues to urinary 3-methylhistidine excretion in the rat. Biochem J (1980) 190, 225228.CrossRefGoogle Scholar
Millward, DJ, Forrester, T, Ah-Sing, E, Yeboah, N, Gibson, N, Badaloo, A, Boyne, M, Reade, M, Persaud, C & Jackson, AThe transfer of 15 N from urea to lysine in the human infant. Br J Nutr (2000) 83, 505512.CrossRefGoogle ScholarPubMed
Millward, DJ & Jackson, AProtein:energy ratios of current diets in developed and developing countries compared with a safe protein:energy ratio: implications for recommended protein and amino acid intakes. Public Health Nutr (2004) 7, 387405.CrossRefGoogle ScholarPubMed
Osborne, T & Mendel, LBThe amino-acid minimum for maintenance and growth as exemplified by further experiments with lysine and tryptophane. J Biol Chem (1916) 25, 112.CrossRefGoogle Scholar
Pencharz, PB, Steffee, WP, Cochran, W, Scrimshaw, NS, Rand, WM & Young, VRProtein metabolism in human neonates: nitrogen-balance studies, estimated obligatory losses of nitrogen and whole-body turnover of nitrogen. Clin Sci Molec Med (1977) 52, 485498.Google ScholarPubMed
Picou, D & Phillips, MUrea metabolism in malnourished and recovered children receiving a high or low protein diet. Am J Clin Nutr (1972) 25, 12611266.CrossRefGoogle ScholarPubMed
Raguso, C, Pereira, P & Young, VRAtracer investigation of the obligatory oxidative amino acid losses in healthy, young adults. Am J Clin Nutr (1999) 70, 474483.CrossRefGoogle ScholarPubMed
Rand, WM, Pellett, PL & Young, VRMeta-analysis of nitrogen balance studies for estimating protein requirements in healthy adults. Am J Clin Nutr (2003) 77, 109127.CrossRefGoogle ScholarPubMed
Rand, WM, Scrimshaw, NS & Young, VRAn analysis of temporal patterns in urinary nitrogen excretion of young adults receiving constant diets at two nitrogen intakes for 8 to 11 weeks. Am J Clin Nutr (1979) 32, 14081414.CrossRefGoogle ScholarPubMed
Rand, WM & Young, VRStatistical analysis of N balance data with reference to the lysine requirement in adults. J Nutr (1999) 129, 19201926.CrossRefGoogle Scholar
Rikimaru, T, Fujita, Y, Okuda, T, Kajiwara, N, Date, C, Heywood, PF, Alpers, MP & Koishi, HUtilization of urea nitrogen in Papua New Guinea highlanders. J Nutr Sci Vitaminol (Tokyo) (1984) 31, 393402.CrossRefGoogle Scholar
Rose, WCThe amino acid requirements of adult man. Nutr Abstr Rev (1957) 27, 631647.Google ScholarPubMed
Scrimshaw, NSW.O. Atwater Memorial Lecture. Through a glass darkly: discerning the practical implications of human dietary protein–energy interrelationships. Nutr Rev (1977) 35, 321336.CrossRefGoogle Scholar
Scrimshaw, NSHuman protein requirements: a brief update. Food Nutr Bull (1996) 17, 185190.CrossRefGoogle Scholar
Scrimshaw, NS, Hussein, MA, Murray, E, Rand, WM & Young, VRProtein requirements of man: variations in obligatory urinary and fecal nitrogen losses in young men. J Nutr (1972) 102, 15951604.CrossRefGoogle ScholarPubMed
Scrimshaw, NS, Wayler, AH, Murray, E, Steinke, FH, Rand, WM & Young, VRNitrogen balance response in young men given one of two isolated soy proteins or milk proteins. J Nutr (1983) 113, 24922497.CrossRefGoogle ScholarPubMed
Sheng, YB, Badger, TM, Asplund, JM & Wixom, RLIncorporation of 15 NH 4 Cl into histidine in adult man. J Nutr (1977) 107, 621630.CrossRefGoogle Scholar
Steffee, WP, Goldsmith, RS, Pencharz, PB, Scrimshaw, NS & Young, VRDietary protein intake and dynamic aspects of whole body nitrogen metabolism in adult humans. Metabolism (1976) 25, 281297.CrossRefGoogle ScholarPubMed
Sukhatme, PV & Margen, SModels for protein deficiency. Am J Clin Nutr (1978) 31, 12371256.CrossRefGoogle ScholarPubMed
Torrallardona, D, Harris, CI & Fuller, MFLysine synthesized by the gastrointestinal microflora of pigs is absorbed, mostly in the small intestine. Am J Physiol (2003) 284, E1177E1180.Google ScholarPubMed
Torun, B, Young, VR & Rand, WMProtein energy requirement studies in developing countries: evaluation of new data. Food Nutr Bull Suppl. (1981) Suppl. 5.Google Scholar
Uauy, R, Scrimshaw, NS & Young, VRHuman protein requirements: nitrogen balance response to graded levels of egg protein in elderly men and women. Am J Clin Nutr (1978 a) 31, 779785.CrossRefGoogle ScholarPubMed
Uauy, R, Winterer, JC, Bilmazes, C, Haverberg, LN, Scrimshaw, NS, Munro, HN & Young, VRThe changing patterns of whole body protein metabolism in aging humans. J Gerontol (1978 b) 33, 663671.CrossRefGoogle ScholarPubMed
United Nations International Action to Avert the Impending Protein Crisis. Report to the Economic and Social Council of the Advisory Committee on the Application of Science and Technology to Development New York: United Nations. (1968).Google Scholar
Waterlow, JCThe nature and significance of nutritional adaptation. Eur J Clin Nutr (1999) 53, Suppl 1 S2S5.CrossRefGoogle ScholarPubMed
Waterlow, JC, Garlick, PJ & Millward, DJProtein Turnover in Mammalian Tissues and the Whole Body Amsterdam: Elsevier/North-Holland Biomedical Press. (1978).Google Scholar
Waterlow, JC & Payne, PRThe protein gap. Nature (1975) 258, 113117.CrossRefGoogle ScholarPubMed
Wayler, A, Quieroz, E, Scrimshaw, NS, Steinke, FH, Rand, WM & Young, VRNitrogen balance studies in young men to assess the protein quality of an isolated soy protein in relation to meat proteins. J Nutr (1983) 113, 24852491.CrossRefGoogle ScholarPubMed
Williams, CDA nutritional disease of children associated with a maize diet. Lancet (1935) ii, 11511152.CrossRefGoogle Scholar
Winterer, JC, Steffee, WP, Davy, W, Perera, A, Uauy, R, Scrimshaw, NS & Young, VRWhole body protein turnover in aging man. Exp Gerontol (1976) 11, 7987.CrossRefGoogle ScholarPubMed
Young, VRRole of skeletal and cardiac muscle in the regulation of body protein metabolism. In Mammalian Protein Metabolism, (1970) Vol 4. Chapter 40 [Munro, HN, editors] New York: Academic Press, Inc.Google Scholar
Young, VRProtein and amino acid requirements in humans: metabolic basis and current recommendations. Scand J Nutr: Naringsforkning (1992) 36, 4756.Google Scholar
Young, VRAdult amino acid requirements: the case for a major revision in current recommendations. J Nutr (1994) 124, 1517S1523S.CrossRefGoogle Scholar
Young, VR, Bier, DM & Pellett, PLA theoretical basis for increasing current estimations of the amino acid requirements in adult man, with experimental support. Am J Clin Nutr (1989) 50, 8092.CrossRefGoogle ScholarPubMed
Young, VR & Borgonha, SNutritional adaptation (genetic, physiological and behavioral): implications for requirements. In From Nutritional Science to Nutrition Process for Better Global Health, Pp. 57160 [Fitzpatrick, DW, Anderson, JE and L'Abbé, ML, editors] Ottawa: Canadian Federation of Biological Societies. (1998).Google Scholar
Young, VR & Borgonha, SNitrogen and amino acid requirements: the Massachusetts Institute of Technology amino acid requirement pattern. J Nutr (2000) 130, 1841S1849S.CrossRefGoogle ScholarPubMed
Young, VR, Chen, SC & Macdonald, JThe sedimentation of rat skeletal-muscle ribosomes: effect of hydrocortisone, insulin and diet. Biochem J (1968 a) 106, 913919.CrossRefGoogle ScholarPubMed
Young, VR & El-Khoury, AECan amino acid requirements for nutritional maintenance in adult humans be approximated from the amino acid composition of body mixed proteins?. Proc Natl Acad Sci USA (1995) 92, 300304.CrossRefGoogle ScholarPubMed
Young, VR, Fajardo, L, Murray, E, Rand, WM & Scrimshaw, NSProtein requirements of man: comparative nitrogen balance response within the submaintenance to maintenance range of intakes of wheat and beef proteins. J Nutr (1975 a) 105, 534542.CrossRefGoogle ScholarPubMed
Young, VR, Gucalp, C, Rand, WM, Matthews, DE & Bier, DMLeucine kinetics during three weeks at submaintenance-to-maintenance intakes of leucine in men: adaptation and accommodation. Hum Nutr Clin Nutr (1987) 41, 118.Google ScholarPubMed
Young, VR, Hussein, MA & Scrimshaw, NSEstimate of loss of labile body nitrogen during acute protein deprivation in young adults. Nature (1968 b) 218, 568569.CrossRefGoogle ScholarPubMed
Young, VR & Munro, HNNτ-methylhistidine (3-methylhistidine) and muscle protein turnover. An overvewi. Fed Proc (1978) 37, 22912300.Google Scholar
Young, VR & Scrimshaw, NSEndogenous nitrogen metabolism and plasma amino acids in young adults given a 'protein-free' diet. Br J Nutr (1968) 22, 920.CrossRefGoogle Scholar
Young, VR, Steffee, WP, Pencharz, PB, Winterer, JC & Scrimshaw, NSTotal human body protein synthesis in relation to protein requirements at various ages. Nature (1975 b) 253, 192194.CrossRefGoogle ScholarPubMed
Young, VR, Taylor, YSM, Rand, WM & Scrimshaw, NSProtein requirements of man: efficiency of egg protein utilization at maintenance and submaintenance levels in young men. J Nutr (1973) 103, 11641174.CrossRefGoogle ScholarPubMed
Young, VR, Wayler, A, Garza, C, Steinke, FH, Murray, E, Rand, WM & Scrimshaw, NSA long-term metabolic balance study in young men to assess the nutritional quality of an isolated soy protein and beef proteins. Am J Clin Nutr (1984) 39, 815.CrossRefGoogle ScholarPubMed
Zhao, X-H, Wen, ZM, Meredith, CN, Matthews, DE, Bier, DM & Young, VRThreonine kinetics at graded threonine intakes in young men. Am J Clin Nutr (1986) 43, 795802.CrossRefGoogle ScholarPubMed