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Utilization of the nucleic acids of Escherichia coli and rumen bacteria by sheep

Published online by Cambridge University Press:  24 July 2007

R. C. Smith ,
N. M. Moussa  and
G. E. Hawkins
R. C. Smith
Affiliation:
Department of Animal and Dairy Sciences, Auburn University, Agricultural Experiment Station, Auburn, Alabama 36830, USA
N. M. Moussa
Affiliation:
Department of Animal and Dairy Sciences, Auburn University, Agricultural Experiment Station, Auburn, Alabama 36830, USA
G. E. Hawkins
Affiliation:
Department of Animal and Dairy Sciences, Auburn University, Agricultural Experiment Station, Auburn, Alabama 36830, USA
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Abstract

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1. Escherichia coli and mixed cultures of rumen bacteria were grown with [8-14C]adenine to label their nucleic acids specifically.

2. The labelled bacteria were injected into the rumen of sheep and the radioactivity incorporated into tissue nucleic acids and that excreted in the urine and faeces was determined.

3. The radioactivity was present in the cold trichloroacetic acid-soluble fraction and the RNA and DNA fractions of all tissues examined. Liver, kidney, spleen, and blood had the highest levels of radioactivity.

4. The radioactivity of the RNA was present only in adenosine monophosphate and guanosine monophosphate.

Type
General Nutrition
Information
British Journal of Nutrition , Volume 32 , Issue 3 , November 1974 , pp. 529 - 537
Copyright
Copyright © The Nutrition Society 1974

References

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