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Use of Tween 40 and Tween 80 to deliver a mixture of phytochemicals to human colonic adenocarcinoma cell (CaCo-2) monolayers

Published online by Cambridge University Press:  09 March 2007

Sinead M. O'Sullivan ,
Julie A. Woods  and
Nora M. O'Brien
Sinead M. O'Sullivan
Affiliation:
Department of Food and Nutritional Sciences, University College, Cork, Republic of Ireland
Julie A. Woods
Affiliation:
Photobiology Unit, University of Dundee, Ninewells Hospital, Dundee DD1 9SY, Scotland, UK
Nora M. O'Brien*
Affiliation:
Department of Food and Nutritional Sciences, University College, Cork, Republic of Ireland
*
*Corresponding author: Dr Nora M. O'Brien, fax +353 21 4270244, email [email protected]
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Abstract

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Epidemiological evidence suggests that dietary intake of carotenoids and tocopherols may influence the risk of certain chronic diseases, such as cancer and CVD. In vitro studies investigating the synergistic effects of mixtures of carotenoids and tocopherols have been hindered due to the difficulty of solubilising these lipophilic compounds. The objective of the present study was to develop a system for delivering tocopherols and carotenoids simultaneously to cells in culture. Differentiated human colonic adenocarcinoma cells (CaCo-2) were incubated with a mixture of these phytochemicals for 24 h. The phytochemical mixture included carotenoids (astaxanthin, canthaxanthin, lutein, lycopene, α-carotene, β-carotene) and tocopherols (α-tocopherol and γ-tocopherol). The emulsifiers polyoxyethylene sorbitan monopalmitate (Tween 40) and polyoxyethylene sorbitan monooleate (Tween 80) were employed as the delivery vehicles, and were compared with tetrahydrofuran (THF). Each vehicle was added at a maximum concentration of 1 ml/l. No toxic effects to the CaCo-2 cells were noted when Tween 40 or Tween 80 were used. Both Tween 40 and Tween 80 resulted in greater solubility of the mixture and delivered substantially more carotenoids and tocopherols to the cells than THF. In particular, lycopene was detected within the cells when Tween 40 and Tween 80 were employed, whereas it was below the limits of detection by HPLC when THF was used as the delivery vehicle. The phytochemicals were retained within the cells for 24 h after supplementation. Tween 40 and Tween 80 have potential as simple, rapid and non-toxic methods for delivering mixtures of carotenoids and tocopherols to cells in culture.

Keywords

CarotenoidsPolyoxyethylene sorbitan estersTweenTetrahydrofuranCaCo-2 cells
Type
Research Article
Information
British Journal of Nutrition , Volume 91 , Issue 5 , May 2004 , pp. 757 - 764
Copyright
Copyright © The Nutrition Society 2004

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