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Use of manufactured foods enriched with fish oils as a means of increasing long-chain n−3 polyunsaturated fatty acid intake

Published online by Cambridge University Press:  09 March 2007

J. A. Lovegrove
Affiliation:
Hugh Sinclair Unit of Human Nutrition, Department of Food Science and Technology, The University of Reading, Whiteknights, Reading RG6 6AP
C. N. Brooks
Affiliation:
Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, GuildfordGU2 5XH
M. C. Murphy
Affiliation:
Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, GuildfordGU2 5XH
B. J. Gould
Affiliation:
Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, GuildfordGU2 5XH
C. M. Williams
Affiliation:
Hugh Sinclair Unit of Human Nutrition, Department of Food Science and Technology, The University of Reading, Whiteknights, Reading RG6 6AP
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Abstract

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The objectives of the present study were to determine the feasibility of using manufactured foods, enriched with eicosapentaenoic acid (EPA) and docosahexaenoic acid(DHA) as a means of increasing the intake of these n−3 polyunsaturated fatty acids (PUFA), and to determine the effect of the consumption of these foods on postprandial lipaemia and other metabolic responses to a high-fat mixed test meal. Nine healthy, normotriacylglycerolaemic, free-living male volunteers (aged 35–60 years) completed the randomized, controlled, single-blind, crossover study. The study consisted of two periods (each of 22d) of dietary intervention, separated by a 5-month washout period. During these two periods the subjects were provided with the manufactured foods enriched with EPA and DHA (n−3 enriched) or identical but unenriched foods (control). A mixed test meal containing 82g fat was given to the fasted subjects on day 22 of each dietary intervention period. Two fasting, and thereafter hourly, blood samples were collected from the subjects for an 8h period postprandially. Plasma triacylglycerol, total and HDL-cholesterol, non-esterified fatty acids (NEFA), glucose and immunoreactive insulin levels, post-heparin lipoprotein lipase (EC 3.1.1.34) activity and the plasma free fatty acid and phospholipid fatty acid compositions were measured. A mean daily intake of 1·4 g EPA + DHA (0·9 g EPA, 0·5 g DHA) was ingested during the n−3-enriched dietary period, which was significantly higher than the intake during the habitual and control periods (P <0·001) assessed by a 3 dweighed food intake. A significantly higher level of EPA + DHA enrichment of the plasma fatty acids and phospholipids (P < 0·001) after the n−3-enriched compared withthe control intervention periods was also found. The energy intake on both of the dietary intervention periods was found to be significantly higher than on the habitual diet (P <0·001), with an increase in body weight of the subjects, which reachedsignificance during the n−3 PUFA-enriched dietary intervention period (P < 0·04). The palatability of the enriched foods was not significantly different from that of the control foods. Significantly higher fasting plasma HDL-cholesterol and glucose concentrations were found after the n-3 PUFA-enriched compared with the control intervention period (P < 0·02 and P < 0·05 respectively). No significant differences were found for the postprandial lipid and hormone measurements, except for significantly lower levels of NEFA at 60min after the n−3-enriched intervention period (P< 0·04). Enriched manufactured foods were a feasible vehicle for increasing n−3 PUFA intake. However the nature of the foods provided as the n−3 vehicle may have contributed to the increased body weight and higher energy intakes which were adverse consequences of the intervention. These factors, together with the short duration of the study may have been reponsible for the failure to observe significant plasma triacylglycerol reductions in response to daily intakes of 1·4g EPA+DHA.

Type
Human and Clinical Nutrition
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Abbey, M., Clifton, P., Kestin, M., Belling, B. & Nestel, P. (1990). Effects of fish-oil on lipoproteins, lecithin: cholesterol acyltransferase and lipid transfer proteins activity in humans. Arteriosclerosis 85, 8594.CrossRefGoogle Scholar
Bang, H. O. & Dyerberg, J. (1972). Plasma lipids and lipoproteins in Greenlandic West-coast Eskimos. Acta Medica Scandinavica 4, 210217.Google Scholar
Bingham, S. A. (1987). The dietary assessment of individuals: methods, accuracy, new techniques and recommendations. Nutrition Abstracts and Reviews 57A, 705742.Google Scholar
Bjerregaard, P. & Dyerberg, J. (1988). Mortality from ischemic heart disease and cerebrovascular disease in Greenland. International Journal of Epidemiology 17, 514519.CrossRefGoogle ScholarPubMed
Blonk, M. C., Bilo, H. J. G., Nauta, J. J. P., Popp-Snijders, C., Mulder, C. & Donker, A. J. M. (1990). Doseresponse effects of fish-oil supplementation in healthy volunteers. American Journal of Clinical Nutrition 52, 120127.CrossRefGoogle ScholarPubMed
Borkman, M., Chisholm, D. J., Furler, S. M., Storlien, L. H., Kraegen, E. W., Simons, L. A. & Chesterman, C. N. (1989). Effects of fish-oil supplementation on glucoseand lipid metabolism in NIDDM. Diabetes 38, 13141319.CrossRefGoogle Scholar
Conner, W. A., Neuringer, M. & Reisbick, S. (1991). Essentiality of n-3 fatty acids:evidence from the primate model and implications for human nutrition. In Health Efsects of n-3 Polyunsaturated Fatty Acids in Seafoods. World Review of Nutrition and Dietetics, vol. 66, pp. 118132 [Simopoulos, A. P., Kifer, R. R., Martin, R. E. and Barlow, S. M., editors]. Basel: Karger.Google Scholar
Davidson, D. M. & Gold, K. V. (1988). Cardiovascular effects of n−3 fatty acids. New England Journal of Medicine 321, 580.Google Scholar
Department of Health (1994). Diet and risk. In Nutritional Aspects of Cardiovascular Disease. Report on Health and Social Subjects, no. 46, pp. 123144. London: H. M.Stationery Office.Google Scholar
Frayn, K. N., Williams, C. M. & Amer, P. (1996). Are increased plasma non-esterified fatty acid concentrations a risk marker for coronary heart disease and other chronic diseases? Clinical Science 90, 243253.CrossRefGoogle ScholarPubMed
Friday, K. E., Childs, M. T., Tsunehara, C. H., Fujimoto, W. Y., Bierman, E. L. & Ensinck, J. W. (1989). Elevated plasma glucose and lipid metabolism in NIDDM. Diabetes Care 12, 276281.CrossRefGoogle Scholar
Gustafsson, I.-B., Ohrvall, M., Ekstrand, B. & Vessby, B. (1996). Moderate amounts of n−3 fatty acid enriched seafood products are effective in lowering serum triglycerides and blood pressure in healthy subjects. Journal of Human Nutrition and Dietetics 9, 135145.CrossRefGoogle Scholar
Gregory, J., Foster, K., Tyler, H. & Wiseman, M. (1990). The Dietary and Nutritional Survey of British Adults. London: H. M. Stationery Office.Google Scholar
Grundy, S. M. & Mok, H. Y. I. (1976). Chylomicron clearance in normal and hyperlipidaemic men. Metabolism 25, 12251239.CrossRefGoogle Scholar
Hampton, S. (1984). C-peptide of proinsulin: its diagnostic use and a possible physiological role. PhD Thesis, university of Surrey.Google Scholar
Harris, W. S. (1989). Fish oils and plasma lipid and lipoprotein metabolism in humans: a critical review. Journal of Lipid Research 30, 785807.CrossRefGoogle ScholarPubMed
Harris, W. S., Windsor, S. L. & Dujovne, C. A. (1991). Effects of four doses of n−3 fatty acids given to hyperlipidaemic patients for six months. journal of the American college of Nutrition 10, 220227.CrossRefGoogle Scholar
Hirai, A., Hamazaki, T., Terano, T., Nishikawa, T., Tamura, Y. & Kumagai, A. (1980). Eicosapentaenoic acid and platelet function in Japanese. Lancet ii, 11321133.CrossRefGoogle Scholar
Lervang, H.-H., Schmidt, E. B., Moller, J., Svaneborg, N., Varming, K., Hadsen, P. H. & Dyerberg, J. (1993). The effect of low-dose supplementation with n-3 polyunsaturated fatty acids on some risk markers of coronary heart disease. Scandinavian Journal of Clinical and Laboratory Investigation 53, 417423.CrossRefGoogle ScholarPubMed
Li, X. & Steiner, M. (1991). Dose response of dietary fish oil supplementations on platelet adhesion. Arteriosclerosis and Thrombosis 11, 3946.CrossRefGoogle ScholarPubMed
Lindgren, F. T., Jensen, L. C. & Hatch, F. T. (1972). Isolation and quantitative analysis of serum lipoprotein. In Blood Lipids and Lipoproteins: Quantitation, Composition and Metabolism, pp. 181274 [Nelson, G. J., editor]. New York: John Wiley & Sons.Google Scholar
Nilsson-Ehle, P. & Schotz, M. C. (1976). A stable, radioactive substrate emulsion for assay of lipoprotein lipase. Journal of Lipid Research 17, 536541.CrossRefGoogle ScholarPubMed
Pike, C. N., Lovegrove, J. A., Murphy, M. C. & Williams, C. M. (1996). The use of n-3 polyunsaturated fatty acid-enriched foods in a dietary intervention study of middle-aged men. Proceedings of the Nutrition Society 55, 80A.Google Scholar
Roche, H. M. (1995). Postprandial lipaemia and haemostasis. PhD Thesis, University of Dublin.Google Scholar
Saynor, R. & Gillott, T. (1992). Changes in blood lipids and fibrinogen with a note on safety in a long-term study on the effects on n-3 fatty acids in Cubjects receiving fish-oil supclements and followed for seven years. Lipids 27. 533538.CrossRefGoogle Scholar
Schectman, G., Kaul, S., Cherayil, G. D., Lee, M. & Kissebah, A. (1989). Can the hypotriglyceridaemic effect of fish-oil concentrate be sustained? Annals of Internal Medicine 110, 346352.CrossRefGoogle Scholar
Schmidt, E. B., Lervang, H.-H., Varming, K., Madsen, P. & Dyerberg, J. (1992). Long-term supplementation with n-3 fatty acids, I: effect on blood lipids, haemostasis and blood pressure. Scandinavian Journal of Clinical and Laboratory Investigation 52, 221228.CrossRefGoogle ScholarPubMed
Schmidt, E. B., Varming, K., Ernst, E., Madsen, P. & Dyerberg, J. (1990). Dose-response studies on the effect of n−3 polyunsaturated fatty acids on lipids and haemostasis. Thrombosis and Huemostasis 63, 15.Google ScholarPubMed
Vessby, B. & Boberg, M. (1990). Dietary supplementation with n3 fatty acids may impair the glucose homeostasis in patients with non-insulin dependent diabetes mellitus. Journal of Internal Medicine 228, 165171.CrossRefGoogle ScholarPubMed
von Schacky, C., Fisher, S. & Weber, P. C. (1985). Long-term effects of dietary marine ω3 fatty acids upon plasma and cellular lipids, platelet function, and eicosanoid formation in humans. Journal of Clinical Investigation 76, 16261631.CrossRefGoogle ScholarPubMed
Weber, P. C. & Leaf, A. (1991). Cardiovascular effects of omega 3 fatty acids. In Health Esfects of w3 Polyunsaturated Fatty Acids in Seafoods. World Review of Nutrition and Dietetics, vol. 66, pp. 218232 [Simopoulos, S. P., Kifer, R. R., Martin, R. E. and Barlow, S. M., editors]. Basel: Karger.Google Scholar