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The trans-10,cis-12 isomer of conjugated linoleic acid reduces hepatic triacylglycerol content without affecting lipogenic enzymes in hamsters

Published online by Cambridge University Press:  09 March 2007

Amaia Zabala ,
Itziar Churruca ,
M. Teresa Macarulla ,
Víctor M. Rodríguez ,
Alfredo Fernández-Quintela ,
J. Alfredo Martínez  and
María P. Portillo
Amaia Zabala
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
Itziar Churruca
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
M. Teresa Macarulla
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
Víctor M. Rodríguez
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
Alfredo Fernández-Quintela
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
J. Alfredo Martínez
Affiliation:
Department of Physiology and Nutrition, University of Navarra, c/ Irunlarrea s/n, 31008 Pamplona, Spain
María P. Portillo*
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
*
*Corresponding author: fax + 34 945 013014, Email [email protected]
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Abstract

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Conjugated linoleic acid (CLA) refers to the positional and geometric dienoic isomers of linoleic acid. The dietary intake of CLA has been associated with changes in lipid metabolism. The aim of the present work was to assess the effects of the two main isomers of CLA on sterol regulatory element binding protein (SREBP)-1a and SREBP-1c mRNA levels, as well as on mRNA levels and the activities of several lipogenic enzymes in liver. For this purpose hamsters were fed an atherogenic diet supplemented with 5 g linoleic acid, cis-9,trans-11 or trans-10,cis-12 CLA/kg diet for 6 weeks. The trans-10,cis-12 isomer intake produced significantly greater liver weight, but also significantly decreased liver fat accumulation. No changes in mRNA levels of SREBP-1a, SREBP-1c and lipogenic enzymes, or in the activities of these enzymes, were observed. There was no effect of feeding cis-9,trans-11 CLA. These results suggest that increased fat accumulation in liver does not occur on the basis of liver enlargement produced by feeding the trans-10,cis-12 isomer of CLA in hamsters. The reduction in hepatic triacylglycerol content induced by this isomer was not attributable to changes in lipogenesis.

Keywords

Conjugated linoleic acidLipogenesisSterol regulatory element binding proteinLiverHamster
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 3 , September 2004 , pp. 383 - 389
Copyright
Copyright © The Nutrition Society 2004

References

Amemiya-Kudo, M, Shimano, H & Hasty, AHTranscriptional activities of nuclear SREBP-1a, -1c, and -2 to different target promoters of lipogenic and cholesterolgenic genes. J Lipid Res (2002) 43, 1220–1235.CrossRefGoogle Scholar
Azain, MJ, Hausman, DB, Sisk, MB, Flatt, WP & Jewell, DEDietary conjugated linoleic acid reduces rat adipose tissue cell size rather than cell number. J Nutr (2000) 130, 1548–1554.CrossRefGoogle ScholarPubMed
Belury, MA & Kempa-Steczko, AConjugated linoleic acid modulates hepatic lipid composition in mice. Lipids (1997) 32, 199–204.CrossRefGoogle ScholarPubMed
Bradford, MMA rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem (1976) 72, 248–254.CrossRefGoogle ScholarPubMed
Chakrabarty, K & Leveille, GAAcetyl-CoA carboxylase and fatty acid synthase activities in liver and adipose tissue of meal-fed rats. Proc Sci Exp Biol Med (1969) 131, 1051–1054.CrossRefGoogle Scholar
Chang, HC, Seidman, I, Teebor, G & Lane, DHLiver acetyl-CoA carboxylase and fatty acid synthase: relative activities in the normal state and in hereditary obesity. Biochim Biophys Res Comm (1967) 28, 682–686.CrossRefGoogle Scholar
Chin, SF, Storkson, JM, Albright, KJ, Cook, ME & Pariza, MWConjugated linoleic acid is a growth factor for rats as shown by enhanced weight gain and improved feed efficiency. J Nutr (1994) 124, 2344–2349.CrossRefGoogle ScholarPubMed
Clément, L, Poirier, H, Niot, I, Bocher, V, Guerre-Millo, M, Krief, S, Staels, B & Bernard, PDietary trans-10,cis-12 conjugated linoleic acid induces hyperinsulinemia and fatty liver in the mouse. J Lipid Res (2002) 43, 1400–1409.CrossRefGoogle ScholarPubMed
Corbalan, MS, Margareto, J, Martínez, JA & Martí, AHigh-fat feeding reduced muscle uncoupling protein 3 expression in rats. J Physiol Pharmacol (1999) 55, 67–72.Google ScholarPubMed
DeLany, JP, Blohm, F, Truett, AA, Scimeca, JA & West, DBConjugated linoleic acid rapidly reduces body fat content in mice without affecting energy intake. Am J Physiol (1999) 276, R1172–R1179.Google ScholarPubMed
Ealey, KN, El-Sohemy, A & Archer, MCEffects of dietary conjugated linoleic acid on the expression of uncoupling proteins in mice and rats. Lipids (2002) 37, 853–861.CrossRefGoogle ScholarPubMed
Field, FJ, Born, E & Mathur, SNFatty acid flux suppress fatty acid synthesis in hamsters intestine independently of SREBP1 expression. J Lipid Res (2003) 44, 1199–1208.CrossRefGoogle Scholar
Folch, J, Lees, M, Sloane Stanley, GHA simple method for the isolation and purification of total lipides from the animal tissues. J Biol Chem (1957) 226, 497–509.CrossRefGoogle ScholarPubMed
Hamura, M, Yamatoya, H & Kudo, SGlycerides rich in conjugated linoleic acid (CLA) improve blood glucose control in diabetic C57BLKS-lepr db /lepr db mice. J Oleo Sci (2001) 50, 889–894.CrossRefGoogle Scholar
Horton, JD, Bashmakov, Y, Shimomura, I & Shimano, HRegulation of sterol regulatory element binding proteins in livers of fasted and refed mice. Proc Natl Acad Sci USA (1998) 95, 5987–5992.CrossRefGoogle ScholarPubMed
Horton, JD, Goldstein, JL & Brown, MSSREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest (2002) 109, 1125–1131.CrossRefGoogle ScholarPubMed
Hsu, RY & Lardy, HAMalic enzyme. Methods Enzymol (1969) 17, 230–235.CrossRefGoogle Scholar
Kim, HJ, Takahashi, M & Ezaki, OFish oil feeding decreases mature sterol regulatory element-binding protein 1 (SREBP-1) by down-regulation of SREBP-1c mRNA in mouse liver. A possible mechanism for down-regulation of lipogenic enzyme mRNAs. J Biol Chem (1999) 274, 25892–25898.CrossRefGoogle ScholarPubMed
Kim, MR, Park, Y, Albright, KJ & Pariza, MWDifferential responses of hamsters and rats fed high-fat or low-fat diets supplemented with conjugated linoleic acid. Nutr Res (2002) 22, 715–722.CrossRefGoogle Scholar
Kuby, SA & Noltmann, EAGlucose 6-phosphate dehydrogenase from brewers yeast. Methods Enzymol (1966) 9, 116–117.CrossRefGoogle Scholar
Lynen, FYeast fatty acid synthase. Methods Enzymol (1969) 14, 14–17.Google Scholar
Martin, JC, Grégoire, S, Siess, MH, Genby, M, Chardigny, JM, Berdeaux, O, Juanéda, P & Sébédio, JLEffects of conjugated linoleic acid isomers on lipid-metabolizing enzymes in male rats. Lipids (2000) 35, 91–98.CrossRefGoogle ScholarPubMed
Miner, JL, Cederberg, CA, Nielsen, MK, Chen, X & Baile, CAConjugated linoleic acid (CLA), body fat, and apoptosis. Obes Res (2001) 9, 129–134.CrossRefGoogle ScholarPubMed
Moya-Camarena, SY & Belury, MASpecies differences in the metabolism and regulation of gene expresión by conjugated linoleic acid. Nutr Rev (1999) 57, 336–340.CrossRefGoogle Scholar
Osborne, TFSterol regulatory element-binding proteins (SREBPs): key regulators of nutritional homeostasis and insulin action. J Biol Chem (2000) 275, 32379–32382.CrossRefGoogle ScholarPubMed
Pai, JT, Guryev, O, Brown, MS & Goldstein, JLDifferential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins. J Biol Chem (1998) 273, 26138–26148.CrossRefGoogle ScholarPubMed
Pariza, MW, Park, Y & Cook, MEMechanisms of action of conjugated linoleic acid: evidence and speculation. Proc Soc Exp Biol Med (2000) 223, 8–13.Google ScholarPubMed
Park, Y, Albright, KJ, Liu, W, Storkson, JM, Cook, ME & Pariza, MWEffect of conjugated linoleic acid on body composition in mice. Lipids (1997) 32, 853–858.CrossRefGoogle ScholarPubMed
Reeves, PG, Nielsen, FH & Fahey, GC JrAIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76 rodent diet. J Nutr (1993) 123, 1939–1951.CrossRefGoogle Scholar
Roche, HM, Noone, E, Nugent, A & Gibney, MJConjugated linoleic acid: a novel therapeutic nutrient?. Nutr Res Rev (2001) 14, 173–187.CrossRefGoogle Scholar
Rodríguez, VM, Picó, C, Portillo, MP, Macarulla, MT & Palou, ADietary fat source regulated ob gene expression in white adipose tissue of rats under hyperphagic feeding. Br J Nutr (2002) 87, 427–434.CrossRefGoogle ScholarPubMed
Sakono, M, Miyanaga, F, Kawahara, S, Yamauchi, K, Fukuda, N, Watanabe, K, Iwata, T & Sugano, MDietary conjugated linoleic acid reciprocally modifies ketogenesis and lipid secretion by the rat liver. Lipids (1999) 34, 997–1000.CrossRefGoogle ScholarPubMed
Shimano, HSterol regulatory element-binding proteins (SREBPs): transcriptional regulators of lipid synthetic genes. Prog Lipid Res (2001) 40, 439–452.CrossRefGoogle ScholarPubMed
Shimano, H, Horton, JD, Shimomura, I, Hammer, RE, Brown, MS & Goldstein, JLIsoform 1c of sterol regulatory element binding protein is less active than isoform 1a in livers of transgenic mice and in cultured cells. J Clin Invest (1997) 99, 846–854.CrossRefGoogle ScholarPubMed
Shimomura, I, Bashmakov, Y & Horton, JDIncreased levels of nuclear SREBP-1c associated with fatty livers in two mouse models of diabetes mellitus. J Biol Chem (1999) 274, 30028–30032.CrossRefGoogle ScholarPubMed
Stangl, GIHigh dietary levels of conjugated linoleic acid mixture alter hepatic glycerophospholipid class profile and cholesterol-carrying serum lipoproteins of rats. J Nutr Biochem (2000) 11, 184–191.CrossRefGoogle ScholarPubMed
Takahashi, Y, Kushiro, M, Shinohara, K & Ide, TActivity and mRNA levels of enzymes involved in hepatic fatty acid synthesis and oxidation in mice fed conjugated linoleic acid. Biochim Biophys Acta (2003) 1631, 265–273.CrossRefGoogle ScholarPubMed
Terpstra, AH, Beynen, AC, Everts, H, Kocsis, S, Katan, MB & Zock, PLThe decrease in body fat in mice fed conjugated linoleic acid is due to increases in energy expenditure and energy loss in the excreta. J Nutr (2002) 132, 940–945.CrossRefGoogle ScholarPubMed
Tsuboyama-Kasaoka, N, Miyazaki, H, Kasaoka, S & Ezaki, OIncreasing the amount of fat in a conjugated linoleic acid-supplemented diet reduces hipodystrophy in mice. J Nutr (2003) 133, 1793–1799.CrossRefGoogle Scholar
Tsuboyama-Kasaoka, N, Takahashi, M, Tanemura, K, Kim, HJ, Tange, T, Okuyama, H, Kasai, M, Ikemoto, S & Ezaki, OConjugated linoleic acid supplementation reduces adipose tissue by apoptosis and develops lipodystrophy in mice. Diabetes (2000) 49, 1534–1542.CrossRefGoogle ScholarPubMed
Wang, YM, Rahman, SM, Nagao, K, Han, SY, Buang, Y, Cha, JY & Yanagita, TConjugated linoleic acid reduces hepatic microsomal triacylglycerol transfer protein activity and hepatic triacylglycerol mass in obese rats. J Oleo Sci (2003) 52, 129–134.CrossRefGoogle Scholar
Warren, JM, Simon, VA, Bartolini, G, Erickson, KL, Mackey, BE & Kelley, DStrans -10, cis -12 CLA increases liver and decreases adipose tissue lipids in mice: possible roles of specific lipid metabolism genes. Lipids (2003) 38, 497–504.CrossRefGoogle ScholarPubMed
West, DB, DeLany, JP, Camet, P, Blohm, FY, Truett, AA & Scimeca, JEffects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am J Physiol (1998) 275, R667–R672.Google ScholarPubMed
Xu, J, Cho, H, O'Malley, S, Park, JH & Clarke, SDDietary polyunsaturated fats regulate rat liver sterol regulatory element binding proteins-1 and -2 in three distinct stages and by different mechanisms. J Nutr (2002) 132, 3333–3339.CrossRefGoogle ScholarPubMed
Xu, J, Nakamura, MT, Cho, HP & Clarke, SDSterol regulatory element binding protein-1 expression is suppressed by dietary polyunsaturated fatty acids. A mechanism for the coordinate suppression of lipogenic genes by polyunsaturated fats. J Biol Chem (1999) 274, 23577–23583.CrossRefGoogle ScholarPubMed
Yahagi, N, Shimano, H, Hasty, AHA crucial role of sterol regulatory element-binding protein-1 in the regulation of lipogenic gene expression by polyunsaturated fatty acids. J Biol Chem (1999) 274, 35840–35844.CrossRefGoogle ScholarPubMed
Zabala, A, Chávarri, M, Macarulla, MT, Navarro, V, Fernández, A, Rodríguez, V & Portillo, MPEffect of conjugated linoleic acid on hepatic oxidation of fatty acids. Nutrition (2003) 19, 1053 .Google Scholar