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trans-10, cis-12 Conjugated linoleic acid inhibits lipoprotein lipase but increases the activity of lipogenic enzymes in adipose tissue from hamsters fed an atherogenic diet

Published online by Cambridge University Press:  08 March 2007

Amaia Zabala ,
Itziar Churruca ,
Alfredo Fernández-Quintela ,
Víctor M. Rodríguez ,
M. Teresa Macarulla ,
J. Alfredo Martínez  and
María P. Portillo
Amaia Zabala
Affiliation:
Department of Nutrition and Food ScienceUniversity of País VascoPaseo de la Universidad 701006 VitoriaSpain
Itziar Churruca
Affiliation:
Department of Nutrition and Food ScienceUniversity of País VascoPaseo de la Universidad 701006 VitoriaSpain
Alfredo Fernández-Quintela
Affiliation:
Department of Nutrition and Food ScienceUniversity of País VascoPaseo de la Universidad 701006 VitoriaSpain
Víctor M. Rodríguez
Affiliation:
Department of Nutrition and Food ScienceUniversity of País VascoPaseo de la Universidad 701006 VitoriaSpain
M. Teresa Macarulla
Affiliation:
Department of Nutrition and Food ScienceUniversity of País VascoPaseo de la Universidad 701006 VitoriaSpain
J. Alfredo Martínez
Affiliation:
Department of Physiology and NutritionUniversity of Navarrac/Irunlarrea s/n31008 PamplonaSpain
María P. Portillo*
Affiliation:
Department of Nutrition and Food ScienceUniversity of País VascoPaseo de la Universidad 701006 VitoriaSpain
*
*Corresponding author: Dr Maráa P. Portillo, fax +34 945 013014, email [email protected]
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Abstract

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The aim of the present work was to investigate the effects of trans-10,cis-12conjugated linoleic acid (CLA) on the activity and expression of lipogenic enzymes and lipoprotein lipase (LPL), as well as on the expression of transcriptional factors controlling these enzymes, in adipose tissue from hamsters, and to evaluate the involvement of these changes in the body fat-reducing effect of this CLA isomer. Thirty male hamsters were divided into three groups and fed atherogenic diets supplemented with 0 (linoleic group), 5 or 10g trans-10,cis-12CLA/kg diet, for 6 weeks. Body and adipose tissue weights, food intake and serum insulin were measured. Total and heparin-releasable LPL and lipogenic enzyme activities (acetyl-CoA carboxylase (ACC); fatty acid synthase (FAS); glucose-6-phosphate dehydrogenase (G6PDH); and malic enzyme (ME)) were assessed. ACC, FAS, LPL, sterolregulatory element-binding proteins (SREBP-1a), SREBP-1c and PPARγ mRNA levels were alsodetermined by real-time PCR. CLA did not modify food intake, body weight and serum insulin level. CLA feeding reduced adipose tissue weight, LPL activity and expression, and increased lipogenic enzyme activities, despite a significant reduction in ACC and FAS mRNA levels. The expression of the three transcriptional factors analysed (SREBP-1a, SREBP-1c and PPARγ) wasalso reduced. These results appear to provide a framework for partially understanding the reduction in body fat induced by CLA. Inhibition of LPL activity seems to be an important mechanism underlying body fat reduction in hamsters. Further research is needed to better characterizethe effects of CLA on lipogenesis and the role of these effects in CLA action.

Keywords

Conjugated linoleic acidLipogenesisLipoprotein lipaseAdipose tissue
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 6 , June 2006 , pp. 1112 - 1119
Copyright
Copyright © The Nutrition Society 2006

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