Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-25T13:07:50.014Z Has data issue: false hasContentIssue false
  • English
  • Français

Time course of changes in in vitro lipolysis of intra-abdominal fat depots in relation to high-fat diet-induced hepatic steatosis in rats

Published online by Cambridge University Press:  08 March 2007

Pascal Collin ,
Natalie Chapados ,
Elise Dufresne ,
Pierre Corriveau ,
Pascal Imbeault  and
Jean-Marc Lavoie
Pascal Collin
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
Natalie Chapados
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
Elise Dufresne
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
Pierre Corriveau
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
Pascal Imbeault
Affiliation:
School of Human Kinetics, University of Ottawa, 125 University Street Ottawa, KIN 6N5, Ontario, Canada
Jean-Marc Lavoie*
Affiliation:
Département de kinésiologie, Université de Montréal, C.P. 6128, Succ. centre-ville, Montréal, Québec, Canada H3C 3J7
*
*Corresponding author: Dr Jean-Marc Lavoie, fax +1 514 343 2181, email: [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The purpose of the present study was to determine the time course of changes in in vitro lipolysis and in perilipin content (Western blot) in the mesenteric and/or the retroperitoneal fat depots in relation to the development of hepatic steatosis in high-fat diet-fed rats. Female Sprague-Dawley rats were submitted to a high-fat diet (HF diet; 42% as kJ) or a standard diet (SD diet) for 1, 2, 3 or 8 weeks. Fat accretion in the mesenteric and retroperitoneal tissues was higher (P<0·01) in HF diet-fed than in SD diet-fed rats as soon as 1 week after the beginning of the diet. Liver triacylglycerol concentrations were significantly (P<0·01) higher in HF diet-fed than in SD diet-fed rats throughout the experiment, the highest values being reached at week 2 of the diet. Basal and stimulated lipolysis (10−4 to 10−7m-isoproterienol) in the mesenteric and retroperitoneal fat depots was not changed during the first 3 weeks, regardless of the diet. Lipolysis in the mesenteric adipose tissue in the basal and stimulated states was, however, higher (P<0·01) in HF diet-fed than in SD diet-fed rats after 8 weeks of the diets. There were no significant (P>0·05) effects of diet and time on perilipin content of mesenteric tissue. In spite of a rapid fat accretion, the present results do not provide any evidence of a rapid (3 weeks) increase in in vitro lipolysis in intra-abdominal fat depots upon the undertaking of an HF diet at a time where liver lipid infiltration is the most significant.

Keywords

PerilipinPlasma NEFALeptinHepatic steatosisIsoproterenol-induced lipolysis
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 2 , August 2006 , pp. 268 - 275
Copyright
Copyright © The Nutrition Society 2006

References

Bellantani, S, Saccoccio, G, Masutti, F, Croce, LS, Brandi, G, Sasso, F, Cristanini, G & Tiribelli, CPrevalence and risk factors for hepatic steatosis in Northern Italy. Ann Intern Med (2000) 132, 112–117.CrossRefGoogle Scholar
Berger, JJ & Barnard, RJEffect of diet on fat cell size and hormone-sensitive lipase activity. J Appl Physiol (1999) 87, 227–232.CrossRefGoogle ScholarPubMed
Brasaemle, DL, Rubin, B, Harten, IA, Gruia-Gray, J, Kimmel, AR & Londos, CPerilipin A increases triacylglycerol storage by decreasing the rate of triacyglycerol hydrolysis. J Biol Chem (2000) 275, 38486–38493.CrossRefGoogle Scholar
Chitturi, S & Farrell, GCEtiopathogenesis of non-alcoholic steatohepatitis. Semin Liver Dis (2001) 21, 27–41.Google Scholar
Enevoldsen, LH, Stallknecht, B, Fluckey, JD & Galbo, CEffect of exercise training on in vivo lipolysis in intra-abdominal adipose tissue in rats. Am J Physiol Endocrinol Metab (2000) 279, E585–E592.CrossRefGoogle ScholarPubMed
Festi, D, Colecchia, A, Sacco, T, Bondi, M, Roda, E & Marchesini, GHepatic steatosis in obese patients: clinical aspects and prognostic significance. Obes Rev (2004) 5, 27–42.CrossRefGoogle ScholarPubMed
Frayn, KNVisceral fat and insulin resistance–causative or correlative?. Br J Nutr (2000) 83, s71–s77.CrossRefGoogle ScholarPubMed
Frayn, KN & Maycock, PFSkeletal muscle triacylglycerol in the rat: methods for sampling and measurement and studies of biological variability. J Lipid Res (1980) 21, 139–144.CrossRefGoogle ScholarPubMed
Gauthier, M-S, Couturier, K, Charbonneau, A & Lavoie, J-MEffects of introducing physical training in the course of a 16-week high-fat diet regimen on hepatic steatosis, adipose tissue fat accumulation, and plasma lipid profile. Int J Obes (2004) 28, 1064–1071.CrossRefGoogle ScholarPubMed
Gauthier, M-S, Couturier, K, Latour, JG & Lavoie, J-MConcurrent exercise prevents high-fat-diet-induced macrovesicular hepatic steatosis. J Appl Physiol (2003) 94, 217–2134.CrossRefGoogle ScholarPubMed
Gauthier, M-S, Favier, R & Lavoie, J-MTime course of the development of non-alcoholic hepatic steatosis in response to high-fat diet-induced obesity in rats. Br J Nutr (2006) 95, 273–281.CrossRefGoogle ScholarPubMed
Greenberg, A.S, Egan, JJ, Wek, SA, Garty, NB, Blanchette-Mackie, EJ & Londos, CPerilipin, a major hormonally regulated adipocyte specific phosphoprotein associated with the periphery of lipid storage droplets. J Biol Chem (1991) 266, 11341–11346.CrossRefGoogle Scholar
Havel, RJ & Hamilton, RLHepatic catabolism of remnant lipoproteins: where the action is. Arterioscler Thromb Vasc Biol (2004) 24, 213–215.CrossRefGoogle ScholarPubMed
James, O & Day, CNon-alcoholic steatohepatitis. Another disease of affluence. Lancet (1999) 353, 1634–1636.CrossRefGoogle ScholarPubMed
Kraegen, EW, Clark, PW, Jenkins, AB, Daley, EA, Chisholm, DJ & Storlien, LHDevelopment of muscle insulin resistance after liver insulin resistance in high-fat-fed rats. Diabetes (1991) 40, 1397–1403.CrossRefGoogle ScholarPubMed
Large, V & Arner, PRegulation of lypolysis in humans. Pathophysiological modulation in obesity, diabetes, and hyperlipidaemia. Diabetes Metab (1998) 24, 409–418.Google Scholar
Lewis, GF, Carpentier, A, Adeli, K & Giacca, ADisordered fat storage and mobilization in the pathogenesis of insulin resistance and type 2 diabetes. Endocrine Rev (2002) 23, 201–229.Google Scholar
Londos, C, Brasaemle, DJ, Schultz, CJ, Segrest, JP & Kimmel, ARPerilipins, ADRP, and other proteins that associate with intracellular neutral lipid droplets in animal cells. Semin Cell Dev Biol (1999) 10, 51–58.CrossRefGoogle ScholarPubMed
Londos, C, Sztalryd, C, Tansey, JT & Kimmel, ARRole of PAT proteins in lipid metabolism. Biochimie (2005) 87, 45–49.CrossRefGoogle ScholarPubMed
Marchesini, G, Brizi, M, Bianchi, G, Tomassetti, S, Bugianesi, E, Lenzi, M, McCullogh, AJ, Natale, S, Forlani, G & Melchionda, NNonalcoholic fatty liver disease. A feature of the metabolic syndrome. Diabetes (2001) 50, 1844–1850.CrossRefGoogle ScholarPubMed
Miles, JM, Park, YS, Walewicz, D, Russell-Lopez, C, Windsor, S, Isley, WL, Coppack, SW & Harris, WSSystemic and forearm triglyceride metabolism: fate of lipoprotein lipase-generated glycerol and free fatty acids. Diabetes (2004) 53, 521–527.CrossRefGoogle ScholarPubMed
Mottagui-Tabar, S, Ryden, M, Lofgren, P, Faulds, G, Hoffstedt, J, Brookes, AJ, Andersson, I & Arner, PEvidence for an important role of perilipin in the regulation of human adipocyte lipolysis. Diabetologia (2003) 46, 789–797.Google ScholarPubMed
Must, A, Spadano, J, Coakley, EH, Field, AE, Colditz, G & Dietz, WHThe disease burden associated with overweight and obesity. JAMA (1999) 282, 1523–1529.CrossRefGoogle ScholarPubMed
Riemens, SC, Sluiter, WJ & Dullaart, RPEnhanced escape of non-esterified fatty acids from tissue uptake: its role in impaired insulin-induced lowering of total rate of appearance in obesity and type 2 diabetes mellitus. Diabetologia (2000) 43, 416–426.Google Scholar
Rodbell, MMetabolism of isolated fat cells I. Effects of hormones on glucose metabolism and lipolysis. Diabetologia (2000) 43, 416–426.Google Scholar
Rodbell, MMetabolism of isolated fat cells I. Effects of hormones on glucose metabolism and lipolysis. J Biol Chem (1964) 239, 375–380.CrossRefGoogle ScholarPubMed
Samuel, VT, Liu, Z-X, Qu, X, Elder, BD, Bilz, S, Belfroy, D, Romanelli, AJ & Shulman, GIMechanism of hepatic insulin resistance in non-alcoholic fatty liver disease. J Biol Chem (2004) 279, 32345–32353.CrossRefGoogle ScholarPubMed
Scheen, AJ & Luyckx, FHObesity and liver disease. Best Pract Res Clin Endocrinol Met (2002) 16, 703–716.CrossRefGoogle ScholarPubMed
Sniderman, AD, Cianflone, K, Arner, P, Summers, LKM & Frayn, KNThe adipocyte, fatty acid trapping, and atherogenesis. Arterioscler Thromb Vasc Biol (1998) 18, 147–151.CrossRefGoogle ScholarPubMed
Sztalryd, C, Xu, G, Dorward, H, Tansey, JT, Contreras, JA, Kimmel, AR & Londos, CPerilipin A is essential for the translocation of hormone-sensitive lipase during lipolytic activation. J Cell Biol (2003) 161, 1011–1012.CrossRefGoogle ScholarPubMed
Wang, Y, Sullivan, S, Trujillo, M, Lee, M-J, Schneider, S, Brolin, RE, Kang, YH, Werber, Y, Greenberg, AS & Fried, SKPerilipin expression in human adipose tissues: effects of severe obesity gender, and depot. Obes Res (2003) 11, 930–936.CrossRefGoogle ScholarPubMed