Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-30T00:54:20.489Z Has data issue: false hasContentIssue false
  • English
  • Français

The subcellular localization of administered N-acetylneuraminic acid in the brains of well-fed and protein restricted rats

Published online by Cambridge University Press:  09 March 2007

Brian L. G. Morgan  and
Myron Winick
Brian L. G. Morgan
Affiliation:
Institute of Human Nutrition, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
Myron Winick
Affiliation:
Institute of Human Nutrition, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. This study investigated the subcellular localization of injected N-acetylneuraminic acid (NeuNAc) in brain. Forty pregnant rats were distributed into four groups. Two groups were given a 200 g casein/kg diel and the other two groups a 100 g casein/kg diet throughout gestation. One group from each of the low- and high-protein groups were given their respective diets for the first 11 d of lactation. On day 12 of lactation, 2·5 μCi [14]NeuNAc/kg body-weight were injected intraperitoneally into their pups. After 1 h the pups were killed, their brains removed and subjected to subcellular fractionation. On day 16 of lactation the other two groups were similarly treated.

2. In all groups of animals 80% of the [14C]NeuNAc incorporated into the brains was found in the synaptosomalfraction and the remainder distributed among the other subcellular fractions in proportion to their total NeuNA ccontent.

3. These results suggest that NeuNAc exerts its effects on behaviour via the synaptic membrane.

Type
Papers of direct reference to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 46 , Issue 2 , September 1981 , pp. 231 - 238
Copyright
Copyright © The Nutrition Society 1981

References

Bruning, J. L. & Kintz, B. L. (1968). Computational Handbook of Statistics, pp. 12–15. Glenview, Illinois: Scott Foresman and Co.Google Scholar
Brunngraber, E. G., Dekirmenjian, M. & Brown, R. D. (1967). Biochem. J. 103, 73.CrossRefGoogle Scholar
Lapetina, E. G., Soto, E. F. & DeRobertis, E. (1967). Biochim. Biophys. Acta 135, 33.CrossRefGoogle Scholar
Ledeen, R. W. (1978). J. Supramol. Struct. 8, 1.CrossRefGoogle Scholar
Merat, A. & Dickerson, J. W. T. (1974). Biol. Neonate 25, 158.CrossRefGoogle Scholar
Morgan, B. L. G. & Winick, M. (1980 a). J. Nutr. 110, 425.CrossRefGoogle Scholar
Morgan, B. L. G. & Winick, M. (1980 b). J. Nutr. 110, 416.Google Scholar
Salas, M., Diaz, S. & Nieto, A. (1974). Brain Res. 73, 139.CrossRefGoogle Scholar
Warren, L. (1959). J. biol. Chem. 234, 1971.Google Scholar
Weseman, W. (1969). FEBS Lett. 3, 80.CrossRefGoogle Scholar
Weseman, W., Henkel, R. & Marx, R. (1971). Biochem. Pharm. 20, 1961.CrossRefGoogle Scholar
Wolfe, L. S. (1961). Biochem. J. 79, 348.CrossRefGoogle Scholar