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Steroids in the intestinal tract of rats are affected by dietary-fibre-rich barley-based diets

Published online by Cambridge University Press:  09 March 2007

Gerhard Dongowski ,
Mario Huth  and
Erich Gebhardt
Gerhard Dongowski*
Affiliation:
Department of Food Chemistry and Preventive Nutrition, German Institute of Human Nutrition, Potsdam-Rehbrücke, Bergholz-Rehbrücke, Germany
Mario Huth
Affiliation:
Department of Food Chemistry and Preventive Nutrition, German Institute of Human Nutrition, Potsdam-Rehbrücke, Bergholz-Rehbrücke, Germany
Erich Gebhardt
Affiliation:
Institute of Nutritional and Environmental Research, Bergholz-Rehbrücke, Germany
*
*Corresponding author: Dr G. Dongowski, fax +49 33200 88444, email [email protected]
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Abstract

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The aim of the present study was to investigate the influence of dietary-fibre (DF)-rich barley-based diets on bile acids (BA) and neutral sterols (NS) in the intestinal tract of rats. For this purpose, young male Wistar rats (n 50; ten per group) weighing about 67g were fed either a barley-free diet (control group) or diets containing 500g barley meal extrudates/kg or a barley meal–Novelose mixture (groups A–D) for 6 weeks. These barley products contained 7–24g resistant starch/100g and 7–12g (1→3),(1→4)-β-glucan/100g. More steroids were transported towards the lower parts of the intestinal tract when higher concentrations of macromolecular DF were present in the diets (P<0·001). Tauroconjugated and primary BA dominated in the contents of the small intestine. Intense enzymic conversion of BA occurred in the caecum and colon. The fermentation of DF affected indirectly the amount of formed secondary BA. The main BA present in the caecal contents were muricholic acids, hyodeoxycholic acid and cholic acid. The BA spectrum in the colonic contents was different from that in the caecum. A higher concentration of NS appeared in the intestinal contents of the groups fed the barley-based diets than in the controls (P<0·005). The microbial conversion of cholesterol to coprostanol, cholestanone and coprostanone was influenced by the amount and composition of the DF in the gut. DF in the diet may affect the concentration and spectrum of steroids in the intestinal tract. The results are relevant for the discussion of mechanisms behind the cholesterol-lowering effects of DF.

Keywords

Barleyβ-GlucanResistant starchSteroids
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 5 , November 2003 , pp. 895 - 906
Copyright
Copyright © The Nutrition Society 2003

References

Asp, N-G, Van Amelsvoort, JMM & Hautvast, JGAJ (1996) Nutritional implications of resistant starch. Nutr Res Rev 9, 1–31.CrossRefGoogle ScholarPubMed
Bach Knudsen, KE, Jensen, BB & Hansen, I (1993) Digestion of polysaccharides and other major components in the small and large intestine of pigs fed on diets consisting of oat fractions rich in β- d -glucan. Br J Nutr 70, 537–556.CrossRefGoogle Scholar
Baron, SF & Hylemon, PB (2000) Biotransformation of bile acids, cholesterol, and steroid hormones. In Gastrointestinal Microbiology, vol. 1. Gastrointestinal Ecosystems and Fermentations, chapter 13, pp. 470–510 [Mackie, RI and White, BA, editors]. New York: Chapman & Hall.Google Scholar
Bartram, H-P, Englert, S, Scheppach, W et al. (1994) Antagonistic effects of deoxycholic acid and butyrate on epithelial cell proliferation in the proximal and distal human colon. Z Gastroenterol 32, 389–392.Google ScholarPubMed
Berry, CS (1986) Resistant starch: formation and measurement of starch that survives exhaustive digestion with amylolytic enzymes during the determination of dietary fibre. J Cereal Sci 4, 301–314.CrossRefGoogle Scholar
Bowles, RK, Morgan, KR, Furneau, RH & Coles, GD (1996) 13 C CP/MAS NMR study of the interaction of bile acids with barley β- D -glucan. Carbohydr Polym 29, 7–10.CrossRefGoogle Scholar
Braaten, TJ, Wood, PJ, Scott, FW et al. (1994) Oat β-glucan reduces blood cholesterol concentration in hypercholesterolemic subjects. Eur J Clin Nutr 48, 465–474.Google ScholarPubMed
Buhman, KK, Furumoto, EJ, Donkin, SS & Story, JA (1998) Dietary psyllium increases fecal bile acid excretion, total steroid excretion and bile acid synthesis. J Nutr 128, 1199–1203.CrossRefGoogle Scholar
Delaney, B, Nicolosi, RJ, Wilson, TA et al. (2003) β-Glucan fractions from barley and oats are similarly antiatherogenic in hypercholesterolemic Syrian golden hamsters. J Nutr 133, 468–475.CrossRefGoogle ScholarPubMed
Demigne, C, Levrat, MA, Behr, SR, Moudras, C & Remesy, C (1998) Cholesterol-lowering action of guar gum in the rat: changes in bile acids and sterols excretion and in enterohepatic cycling of bile acid. Nutr Res 18, 1215–1225.CrossRefGoogle Scholar
Deutsche Gesellschaft für Ernährung (2000) Referenzwerte für die Nährstoffzufuhr, pp. 59–63. Frankfurt am Main, Germany: Umschau-Braus Verlagsgesellschaft.Google Scholar
Dongowski, G (1995) Influence of pectin structure on the interaction with bile acids under in vitro conditions. Z Lebensm Untersuch Forsch 201, 390–398.CrossRefGoogle ScholarPubMed
Dongowski, G (1997) Effect of pH on the in vitro interactions between bile acids and pectin. Z Lebensm Untersuch Forsch 205, 185–192.CrossRefGoogle Scholar
Dongowski, G & Ehwald, R (1999) Binding of water, oil and bile acids to dietary fibers of the cellan type. Biotechnol Progr 15, 250–258.CrossRefGoogle ScholarPubMed
Dongowski, G, Huth, M, Gebhardt, E & Flamme, W (2002) Dietary fiber-rich barley products beneficially affect the intestinal tract of rats. J Nutr 132, 3704–3714.CrossRefGoogle ScholarPubMed
Doublier, J-L & Wood, PJ (1995) Rheological properties of aqueous solutions of (1→3)(1→4)-β- D -glucan from oats ( Avena sativa L.). Cereal Chem 72, 335–340.Google Scholar
Dvir, I, Chayoth, R, Sod-Moriah, U et al. (2000) Soluble polysaccharide and biomass of red microalga Porphyridium sp. alter intestinal morphology and reduce serum cholesterol in rats. Br J Nutr 84, 469–476.CrossRefGoogle ScholarPubMed
Fadden, K, Hill, MJ & Owen, RW (1997) Effect of fibre on bile acid metabolism by human faecal bacteria in batch and continuous culture. Eur J Cancer Prev 6, 175–194.Google ScholarPubMed
Fernandez, ML, Lin, ECK, Trejo, A & McNamara, DJ (1994) Prickly pear (Opuntia sp.) pectin alters hepatic cholesterol homeostasis without affecting cholesterol absorption in guinea pigs fed a hypercholesterolemic diet. J Nutr 124, 817–824.CrossRefGoogle ScholarPubMed
Fernandez, ML, Sun, DM, Tosca, M & McNamara, DJ (1995) Differential effects of guar gum on LDL and hepatic cholesterol metabolism in guinea pigs fed low and high cholesterol diets. Am J Clin Nutr 61, 127–134.CrossRefGoogle Scholar
Gallaher, DG, Locket, PL & Gallaher, CM (1992) Bile acid metabolism in rats fed two levels of corn oil and brans from oat, rye and barley and sugar beet fiber. J Nutr 122, 473–481.CrossRefGoogle ScholarPubMed
Goel, V, Cheema, SK, Agellon, LB, Ooraikul, B & Basu, TK (1999) Dietary rhubarb (Rheum rhaponticum) stalk fibre stimulates cholesterol 7α-hydroxylase gene expression and bile acids excretion in cholesterol-fed C57BL/6J mice. Br J Nutr 81, 65–71.CrossRefGoogle ScholarPubMed
Hecker, KD, Meier, ML, Newman, RK & Newman, CW (1998) Barley β-glucan is effective as a hypocholesterolaemic ingredient in foods. J Sci Food Agric 77, 179–183.3.0.CO;2-0>CrossRefGoogle Scholar
Hofmann, AF (1994) Intestinal absorption of bile acids and biliary constituents. The intestinal components of the enterohepatic circulation and the integrated system. In Physiology of the Gastrointestinal Tract, 3rd ed., pp, 1845–1865 [Johnson, LR, editor]. New York: Raven Press.Google Scholar
Hofmann, AF (1999) The continuing importance of bile acids in liver and intestinal disease. Arch Intern Med 159, 2647–2658.CrossRefGoogle ScholarPubMed
Hofstad, B, Vatn, MH, Andersen, S, Owen, RW, Larsen, S & Osnes, M (1998) The relationship between faecal bile acid profile with or without supplementation with calcium and antioxidants on recurrence and growth of colorectal polyps. Eur J Cancer Prev 7, 287–294.CrossRefGoogle ScholarPubMed
Hori, T, Matsumoto, K, Sakaitani, Y, Sato, M & Morotomi, M (1998) Effect of deoxycholic acid and cholesterol on faecal steroid concentration and its impact on the colonic crypt cell proliferation in azoxymethane-treated rats. Cancer Lett 124, 79–84.CrossRefGoogle ScholarPubMed
Huth, M, Dongowski, G, Gebhardt, E & Flamme, W (2000) Functional properties of dietary fibre enriched extrudates from barley. J Cereal Sci 32, 115–128.CrossRefGoogle Scholar
Jadhav, SJ, Lutz, SE, Ghorpade, VM & Salhunkhe, DK (1998) Barley: chemistry and value-added processing. Crit Rev Food Sci 38, 123–171.CrossRefGoogle ScholarPubMed
Johansen, HN, Bach Knudsen, KE, Wood, PJ & Fulcher, RG (1997) Physico-chemical properties and the degradation of oat bran polysaccharides in the gut of pigs. J Sci Food Agric 73, 81–92.3.0.CO;2-Z>CrossRefGoogle Scholar
Johansen, HN, Wood, PJ & Bach Knudsen, KE (1993) Molecular weight changes in the (1→3)(1→4)-β- D -glucan of oats incurred by the digestive processes in the upper gastrointestinal tract of pigs. J Agric Food Chem 41, 2347–2352.CrossRefGoogle Scholar
Kahlon, TS, Chow, FI, Knuckles, BI & Chiu, MM (1993) Cholesterol-lowering effects in hamsters of β-glucan-enriched barley fraction, dehulled whole barley, and oat bran and their combinations. Cereal Chem 70, 435–440.Google Scholar
Kalra, S & Jood, S (2001) Effect of dietary barley β-glucan on cholesterol and lipoprotein fractions in rats. J Cereal Sci 32, 141–145.Google Scholar
Kerckhoffs, DAJM, Brouns, F, Hornstra, G & Mensink, RP (2002) Effects on human serum lipoprotein profile of β-glucan, soy protein and isoflavones, plant sterols and stanols, garlic and tocotrienols. J Nutr 132, 2494–2505.CrossRefGoogle ScholarPubMed
Lia, Å, Hallmans, G, Sandberg, A-S, Sundberg, B, Åman, P & Andersson, H (1995) Oat β-glucan increases bile acid excretion and a fiber-rich barley fraction increases cholesterol excretion in ileostomy subjects. Am J Clin Nutr 62, 1245–1251.CrossRefGoogle Scholar
Lifschitz, CH, Grusak, MA & Butte, NF (2002) Carbohydrate digestion in humans from a β-glucan-enriched barley is reduced. J Nutr 132, 2593–2596.CrossRefGoogle ScholarPubMed
Lund, EK, Gee, JM, Brown, JC, Wood, PJ & Johnson, IT (1989) Effect of oat gum on the physical properties of the gastrointestinal contents and on the uptake of d -galactose and cholesterol by rat small intestine in vitro. Br J Nutr 62, 91–101.CrossRefGoogle ScholarPubMed
Lupton, JR, Robinson, MC & Morin, JL (1994) Cholesterol-lowering effect of barley bran flour and oil. J Am Diet Assoc 94, 65–70.CrossRefGoogle ScholarPubMed
McCleary, BV & Mugford, DC (1997) Determination of β-glucan in barley and oat by streamlined enzymatic methods: summary of collaborative study. J Assoc Off Anal Chem Intern 80, 580–583.Google Scholar
McIntosh, GH, Newman, RK & Newman, CW (1995) Barley foods and their influence on cholesterol metabolism. World Rev Nutr Diet 77, 89–108.CrossRefGoogle ScholarPubMed
McIntosh, GH, Whyte, J, McArthur, R & Nestel, PJ (1991) Barley and wheat foods: influence on plasma cholesterol concentrations in hypercholesterolemic men. Am J Clin Nutr 53, 1205–1209.CrossRefGoogle ScholarPubMed
McMillan, L, Butcher, S, Wallis, Y, Neoptolemos, JP & Lord, JM (2000) Bile acids reduce the apoptosis-inducing effects of sodium butyrate in human colon adenoma (AA/C1) cells: implications for colon carcinogenesis. Biochem Biophys Res Commun 273, 45–49.CrossRefGoogle ScholarPubMed
Mälkki, Y & Virtanen, E (2001) Gastrointestinal effects of oat bran and oat gum – a review. Lebensm Wiss Technol 34, 337–347.CrossRefGoogle Scholar
Moghadasian, MH (2000) Pharmacological properties of plant sterols – in vivo and in vitro observations. Life Sci 67, 605–615.CrossRefGoogle ScholarPubMed
Moreau, RA, Whitaker, BD & Hicks, KB (2002) Phytosterols, phytostanols, and their conjugates in foods: structural diversity, quantitative analysis, and health-promoting uses. Progr Lipid Res 41, 457–500.CrossRefGoogle ScholarPubMed
Owen, RW (1997) Faecal steroids and colorectal carcinogenesis. Scand J Gastroenterol 32, Suppl. 222, 76–82.CrossRefGoogle Scholar
Piironen, V, Lindsay, GD, Miettinen, TA, Toivo, J & Lampi, A-M (2000) Plant sterols: biosynthesis, biological function and their importance to human nutrition. J Sci Food Agric 80, 939–966.3.0.CO;2-C>CrossRefGoogle Scholar
Prosky, L, Asp, N-G, Furda, I, de Vries, JW & Schweizer, TF (1988) Determination of insoluble, soluble and total dietary fiber in foods, and foods products: interlaboratory study. J Assoc Off Anal Chem 71, 1017–1023.Google ScholarPubMed
Roberton, AM (1993) Roles of endogenous substances and bacteria in colorectal cancer. Mutat Res 290, 71–78.CrossRefGoogle ScholarPubMed
Robertson, JA, Majsak-Newman, G & Ring, SG (1997) Release of mixed linkage (1→3),(1→4) β- D -glucans from barley by protease activity and effects on ileal effluents. Intern J Biol Macromol 21, 57–60.CrossRefGoogle Scholar
Romero, AL, West, KL, Zern, T & Fernandez, ML (2002) The seeds from Plantago ovata lower plasma lipids by altering hepatic and bile acid metabolism in guinea pigs. J Nutr 132, 1194–1198.CrossRefGoogle ScholarPubMed
Roy, P, Owen, RW, Faivre, J et al. (1999) Faecal neutral sterols and bile acids in patients with adenomas and the large bowel cancer: an ECP case-control study. Eur J Cancer Prev 8, 409–515.CrossRefGoogle ScholarPubMed
Salter, J, Chaplin, M, Dickerson, J & Davies, J (1996) Bile acids and health: is fibre the answer? Nutr Food Sci 6, 29–33.CrossRefGoogle Scholar
Seal, CJ & Mathers, JC (2001) Comparative gastrointestinal and plasma cholesterol responses of rats fed on cholesterol-free diets supplemented with guar gum and sodium alginate. Br J Nutr 85, 317–324.CrossRefGoogle ScholarPubMed
Sundberg, B, Wood, P, Lia, Å et al. (1996) Mixed-linked β-glucan from breads of different cereals is partly degraded in the human ileostomy model. Am J Clin Nutr 64, 878–885.CrossRefGoogle ScholarPubMed
Tejinder, S, Bhupinder, K & Harinder, K (2000) Flow behavior and functional properties of barley and oat water-soluble β- D -glucan rich extractions. Intern J Food Prop 3, 259–274.CrossRefGoogle Scholar
Terpstra, AHM, Lapré, JA, de Vries, HT & Beynen, AC (2000) Hypocholesterolemic effect of dietary psyllium in female rats. Ann Nutr Metabol 44, 223–228.CrossRefGoogle ScholarPubMed
Trautwein, EA, Rieckhoff, D & Erbersdobler, HF (1998) Dietary inulin lowers plasma cholesterol and triacylglycerol and alters biliary bile acid profile in hamsters. J Nutr 128, 1937–1943.CrossRefGoogle ScholarPubMed
Trautwein, EA, Schulz, C, Rieckhoff, D et al. (2002) Effect of esterified 4-desmethysterols and -stanols or 4,4'-dimethylsterols on cholesterol and bile acid metabolism in hamsters. Br J Nutr 87, 227–237.CrossRefGoogle ScholarPubMed
Vanhoof, K & De Schrijver, R (1998) The influence of enzyme-resistant starch on cholesterol metabolism in rats fed on a conventional diet. Br J Nutr 80, 193–198.CrossRefGoogle ScholarPubMed
Vasanthan, T, Jiang, GS, Yeung, J & Li, JH (2002) Dietary fiber profile of barley flour as affected by extrusion cooking. Food Chem 77, 35–40.CrossRefGoogle Scholar
Vranjes, MV & Wenk, C (1995) The influence of extruded vs untreated barley in the feed, with and without dietary enzyme supplement on broiler performance. Anim Feed Sci Technol 54, 21–32.CrossRefGoogle Scholar
Wang, G, Stacey, NH & Earl, J (1990) Determination of individual bile acids in serum by high performance liquid chromatography. Biomed Chromatogr 4, 136–140.CrossRefGoogle ScholarPubMed