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Stability of tryptophan during food processing and storage

2. A comparison of methods used for the measurement of tryptophan losses in processed foods

Published online by Cambridge University Press:  24 July 2007

Henrik K. Nielsen
Affiliation:
Research Department, Nestlé Products Technical Assistance Co. Ltd, CH-1814 La Tour-de-Peilz, Switzerland
A. Klein
Affiliation:
Research Department, Nestlé Products Technical Assistance Co. Ltd, CH-1814 La Tour-de-Peilz, Switzerland
R. F. Hurrell
Affiliation:
Research Department, Nestlé Products Technical Assistance Co. Ltd, CH-1814 La Tour-de-Peilz, Switzerland
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Abstract

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1. Tryptophan losses in stored milk powders and in different model systems representing the major reactions of food proteins during processing and storage were determined using four different chemical methods and in a rat assay.

2. Similar tryptophan values were obtained by the three chemical methods which included high pressure liquid chromatography (HPLC) after sodium hydroxide hydrolysis. colorimetric reaction with p-dimethylamino- benzaldehyde (p-DAB) after barium hydroxide hydrolysis, and fluorescence of the Norharman derivative after NaOH hydrolysis.

3. Tryptophan losses in the treated proteins as measured by the alkaline-hydrolysis methods were generally smaller than those determined by the rat assay. Good agreement however was obtained when the chemical value was multiplied by the true nitrogen digestibility.

4. Determination of tryptophan by reaction with p-DAB after papain (EC 3.4.22.2) digestion gave lower values in the processed proteins than the other chemical methods or the rat assay.

5. A method using alkaline-hydrolysis is recommended, preferably combined with HPLC-measurement of the liberated tryptophan.

Type
Papers on General Nutrition
Copyright
Copyright © The Nutrition Society 1985

References

Boyne, A. W., Ford, J. E., Hewitt, D. & Shrimpton, D. H. (1975). British Journal of Nutrition 34, 153162.CrossRefGoogle Scholar
Buttery, P. J. & Soar, J. B. (1975). Journal of the Science of Food and Agriculture 26, 12731277.Google Scholar
Dworschak, E. & Hegedüs, M. (1974). Acta Alimentaria 3, 337347.Google Scholar
Finot, P. A., Magnenat, E., Guignard, G. & Hurrell, R. F. (1982). International Journal for Vitamin and Nutrition Research 52, 226.Google Scholar
Friedman, M. & Finley, J. W. (1975). In Protein Nutritional Quality of Food and Feeds, part 1, pp. 423452 [Friedman, M., editor]. New York: Marcel Dekker Inc.Google Scholar
Friedman, M., Levin, C. E., Noma, A. T., Montagne, W. C. & Zahnley, J. C. (1984). In Progress in Serotonin and Tryptophan Research, pp. 119123 [Schlossberger, H. G., Kochen, W., Linzen, B. and Steinhart, H., editors]. Berlin: Walter de Gruyter & Co.Google Scholar
Gruen, L. C. & Rivett, D. E. (1971). Analytical Biochemistry 44, 519522.CrossRefGoogle Scholar
Hopkins, D. T. (1981). In Protein Quality in Humans: Assessment and In Vitro Estimation, pp. 169193 [Bodwell, C. E., Adkins, J. S. and Hopkins, D. T., editors]. Westport, Conn.: Avi Publishing Co., Inc.Google Scholar
Hugli, T. E. & Moore, S. (1972). Journal of Biological Chemistry 247, 28282834.Google Scholar
Hurrell, R. F., Carpenter, K. J., Sinclair, W. J., Otterburn, M. S. & Asquith, R. S. (1976). British Journal of Nutrition 35, 383395.Google Scholar
Hurrell, R. F., Finot, P. A. & Ford, J. E. (1983). British Journal of Nutrition 49, 343354.Google Scholar
Inglis, A. S., McMahon, D. T. W., Roxborough, C. M. & Takayanagi, H. (1976). Analytical Biochemistry 72, 8694.Google Scholar
Liu, T.-Y. & Chang, Y. H. (1971). Journal of Biological Chemistry 246, 28422848.Google Scholar
Mauron, J., Mottu, F. & Egli, R. H. (1960). Annales de la Nutrition et de l'Alimentation 14, 135150.Google Scholar
Miller, E. L. (1967). Journal of the Science of Food and Agriculture 18, 381386.CrossRefGoogle Scholar
Nielsen, H. K., de Week, D., Finot, P. A., Liardon, R. & Hurrell, R. F. (1985). British Journal of Nutrition 53, 281292.Google Scholar
Nielsen, H. K. & Hurrell, R. F. (1985). Journal of the Science of Food and Agriculture (In the Press).Google Scholar
Spackman, D. H., Stein, W. H. & Moore, S. H. (1958). Analytical Chemistry 30, 11901206.Google Scholar
Wilkinson, M., Iacobucci, G. A. & Myers, D. V. (1976). Analytical Biochemistry 70 470478.Google Scholar
Williams, A. P., Hewitt, D. & Buttery, P. J. (1982). Journal of the Science of Food and Agriculture 33, 860865.Google Scholar