Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-24T14:10:01.707Z Has data issue: false hasContentIssue false
  • English
  • Français

Short-term ingestion of chlorogenic or caffeic acids decreases zinc but not copper absorption in rats, utilization of stable isotopes and inductively-coupled plasma mass spectrometry technique

Published online by Cambridge University Press:  09 March 2007

Charles Coudray ,
Carole Bousset ,
Jean C. Tressol ,
Denise Pépin  and
Yves Rayssiguier
Charles Coudray*
Affiliation:
Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France
Carole Bousset
Affiliation:
Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France
Jean C. Tressol
Affiliation:
Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France
Denise Pépin
Affiliation:
Laboratoire d'hydrologie, Institut Louise Blanquet, Faculté de Pharmacie, Université d'Auvergne, Clermont-Ferrand, France
Yves Rayssiguier
Affiliation:
Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France
*
*Dr Charles Coudray, fax +33 04 73 62 46 38, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The amount of dietary trace elements absorbed from a meal depends, among other factors, on the quantities of certain minor plant constituents present in the meal. These substances can act as ligands and bind trace elements in the digestive tract in available or unavailable forms for absorption. The present study was designed to investigate the extent to which different polyphenols (PP) may influence Zn and Cu absorption in rats. Different PP of nutritional interest (chlorogenic acid, caffeic acid, catechin and rutin) were studied using meals extrinsically-labelled with stable isotopes67Zn and 65Cu. Male Wistar rats were fed on a non-labelled semi-synthetic diet containing (mg/kg) 38 Fe, 35 Zn and 7·5 Cu for 8 d. PP were dissolved in dimethyl sulfoxide as the solvent and added to the meal at 1 g/kg during 3 d before isotope administration and until the end of the experiment (a further 3 d). The control group received the dimethyl sulfoxide only. After overnight food deprivation, rats were fed on the labelled test meals (4 g diet+0·1 mg 67Zn and 0·1 mg 65Cu) with 0·5 mg Dy as a faecal marker. Faeces and urine pools were collected for 3 d and analysed for 67Zn and65 Cu isotopic enrichment using the inductively-coupled plasma mass spectrometry technique. Zn absorption was significantly less in rats fed on chlorogenic acid or caffeic acid than in the control group. Catechin ingestion non-significantly inhibited 67Zn absorption. However, the PP studied were without effect on Cu absorption. The study illustrates the effect of metal-binding phenolic compounds on mineral nutrition in the rat, and the possible importance of the effects of different foods rich in these compounds on mineral absorption in man.

Keywords

PolyphenolsCu absorptionZn absorptionIsotope technique
Type
Research Article
Information
British Journal of Nutrition , Volume 80 , Issue 6 , December 1998 , pp. 575 - 584
Copyright
Copyright © The Nutrition Society 1998

References

American Institute of Nutrition (1977) Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Arnaud, J, Chappuis, P, Jaudon, MC & Bellanger, J (1993) Marqueurs biologiques nutritionnels des carences en zinc, cuivre et sélénium (Biological nutritional markers of deficiencies in zinc, copper and selenium). Annals de Biologie Clinique 51, 589–604.Google Scholar
Brown, RC, Klein, A, Simmons, WK & Hurrell, RF (1990) The influence of Jamaican herb teas and other polyphenol-containing beverages on iron absorption in the rat. Nutrition Research 10, 342–353.Google Scholar
Brune, M, Rossander, L & Hallberg, L (1989) Iron absorption and phenolic compounds; importance of different phenolic structures. European Journal of Clinical Nutrition 43, 547–558.Google ScholarPubMed
Cook, JD, Reddy, MD & Hurrell, RF (1995) The effect of red and white wine on nonheme-iron absorption in humans. American Journal of Clinical Nutrition 61, 800–804.CrossRefGoogle ScholarPubMed
Cook, NC & Samman, S (1996) Flavonoids: Chemistry, metabolism, cardioprotective effects and dietary sources. Journal of Nutritional Biochemistry 7, 66–76.CrossRefGoogle Scholar
Coudray, C & Fairweather-Tait, S (1988) Do oligosaccharides affect intestinal absorption in humans? American Journal of Clinical Nutrition (In the press).Google Scholar
Coudray, C, Pépin, D, Tressol, JC, Bellanger, J & Rayssiguier, Y (1997) Study of magnesium bioavailability using stable isotopes and the inductively-coupled plasma mass spectrometry technique in the rat: single and double labelling approaches. British Journal of Nutrition 77, 957–970.Google Scholar
De Bièvre, P & Taylor, PDP (1993) Table of isotopic composition of the elements. International Journal of Mass Spectrometry Ion Process 123, 149–166.Google Scholar
Disler, PB, Lynch, SR, Charlton, RW, Torrance, JD, Bothwell, TH, Walker, RB & Mayet, F (1975) The effect of tea on iron absorption. Gut 16, 193–200.Google Scholar
EEC (1986) Guidelines for the Use of Experimental Animals. L358-86/609/EEC. Brussels: Commission of European Community.Google Scholar
Fairweather-Tait, SJ, Minihane, AM, Eagles, J, Owen, L & Crews, HM (1997) Rare earth elements as non-absorbable fecal markers in studies of iron absorption. American Journal of Clinical Nutrition 65, 970–976.CrossRefGoogle Scholar
Fairweather-Tait, SJ & Piper, Z (1991) The effect of tea on iron and aluminium metabolism in the rat. British Journal of Nutrition 65, 61–68.Google Scholar
Flanagan, PR, Cluett, J, Chamberlain, MJ & Valberg, LS (1985) Dual-isotope method for determination of human zinc absorption: the use of a test meal of turkey meat. Journal of Nutrition 115, 111–122.Google Scholar
Gabrielli, GB & De, Sandre G (1995) Excessive tea consumption can inhibit the efficacy of oral iron treatment in iron-deficiency anemia. Haematologica 80, 518–520.Google ScholarPubMed
Ganji, V & Kies, CV (1994) Zinc bioavailability and tea consumption: study in healthy humans consuming self-selected and laboratory-controlled diets. Plant Foods for Human Nutrition 46, 267–276.Google Scholar
Garcia-Lopez, JS, Erdman, JW & Sherman, AR (1990) Iron retention by rats from casein-legume test meals: Effect of tannin level and previous diet. Journal of Nutrition 120, 760–766.Google Scholar
Greger, JL & Lyle, BJ (1988) Iron, copper and zinc metabolism of rats fed various levels and types of tea. Journal of Nutrition 118, 52–60.Google Scholar
Gutnisky, A, Rizzo, N, Castro, ME & Garbossa, G (1992) The inhibition action of chlorogenic acid on the intestinal iron absorption in rats. Acta Physiologica et Pharmacologica Latinoamericana 42, 139–146.Google Scholar
Hertog, MGL & Hollman, PCH (1996) Potential health effects of the dietary flavonol quercetin. European Journal of Clinical Nutrition 50, 63–71.Google ScholarPubMed
Hurrell, RF (1990) The influence of polyphenol-containing beverages on iron absorption. Proceedings of XVth International Meeting on Polyphenols, Strasbourg, Vol. 15, pp. 268–274.Google Scholar
Janghorbani, M & Ting, BTG (1990) Stable isotope methods for studies of mineral/trace element metabolism. Journal of Nutritional Biochemistry 1, 4–19.Google Scholar
McDonald, M, Mila, I & Scalbert, A (1996) Precipitation of metal ions by plant polyphenols: optimal conditions and origin of precipitation. Journal of Agricultural and Food Chemistry 44, 599–606.CrossRefGoogle Scholar
Morck, TA, Lynch, SR & Cook, JD (1983) Inhibition of food iron absorption by coffee. American Journal of Clinical Nutrition 37, 416–420.CrossRefGoogle ScholarPubMed
Record, IR, McInerney, JK & Dreosti, IE (1996) Black tea, green tea, and tea polyphenols: effects on trace element status in weaning rats. Biological Trace Element Research 5, 27–43.CrossRefGoogle Scholar
Reddy, NR, Pierson, MD, Sathe, SK & Salunkhe, DK (1985) Dry bean tannins: a review of nutritional implications. Journal of the American Oil Chemistry Society 62, 541–549.CrossRefGoogle Scholar
Schuette, SA, Janghorbani, M, Young, VR & Weaver, CM (1993) Dysprosium as a nonabsorbable marker for studies of mineral absorption with stable isotope tracers in human subjects. Journal of the American College of Nutrition 12, 307–312.CrossRefGoogle ScholarPubMed
Spencer, CM, Gai, Y, Martin, R, Gaffney, SH, Goulding, PN, Magnolato, D, Lilley, TH & Haslam, E (1988). Polyphenol complexation. Some thoughts and observations. Phytochemistry 27, 2397–2409.Google Scholar
Statistical Analysis Systems (1989) SAS Users Guide. Statistics, 5th ed. pp. 891–996. Cary, NC: SAS Institute.Google Scholar
Tuntawiroon, M, Sritongkul, N, Brune, M, Rossander-Hultén, L, Pleehachinda, R, Suwanik, R & Hallberg, L (1991) Dose-dependent inhibitory effect of phenolic compounds in foods on non heme-iron absorption in men. American Journal of Clinical Nutrition 53, 554–557.Google Scholar
Veillon, C & Patterson, KY (1996) Trace elements in a commercial freeze-dried human urine reference material. Analyst 121, 983–985.Google Scholar