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Rumen protein degradation and biosynthesis

1. A new method for determination of protein degradation in rumen fluid in vitro

Published online by Cambridge University Press:  24 July 2007

L. Raab ,
B. Cafantaris ,
T. Jilg  and
K. H. Menke
L. Raab
Affiliation:
Institute of Animal Nutrition, University of Hohenheim, Emil-Wolff-Strasse 10, 7000 Stuttgart 70, Federal Republic of Germany
B. Cafantaris
Affiliation:
Institute of Animal Nutrition, University of Hohenheim, Emil-Wolff-Strasse 10, 7000 Stuttgart 70, Federal Republic of Germany
T. Jilg
Affiliation:
Institute of Animal Nutrition, University of Hohenheim, Emil-Wolff-Strasse 10, 7000 Stuttgart 70, Federal Republic of Germany
K. H. Menke
Affiliation:
Institute of Animal Nutrition, University of Hohenheim, Emil-Wolff-Strasse 10, 7000 Stuttgart 70, Federal Republic of Germany
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Abstract

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1. A method is described for the determination of protein degradation based on measurements of ammonia concentration and gas production (Menke et al. 1979) when a feedingstuff was incubated with rumen fluid in vitro.

2. NH3 liberated during incubation is in part used for microbial protein synthesis. Production of carbon dioxide and methane can be regarded as a measure of energy available for protein synthesis. The ratio, gas production: incorporation of NH3-nitrogen was estimated by addition of starch to the substrate. The response in gas production was linear in the range 0–200 mg starch, when starch was added to 0–200mg feedingstuff dry matter and 30 ml rumen fluid-medium mixture.

3. Linear regression between NH3-N concentration (y, mg) and gas production (x, ml) yielded an intercept (b0) representing that amountof NH3-N which would be released when no fermentable carbohydrates were available and consequently no bacterial protein synthesis took place.

4. The difference between this intercept b0 and NH3-N content in the blank (rumen fluid without substrate added) indicated the amount of NH3 liberated from protein and other N-containing compounds of the feedingstuff incubated. In vitro-degradable N (IVDN) was calculated as a proportion of total N by the equation:

Type
Research Article
Information
British Journal of Nutrition , Volume 50 , Issue 3 , November 1983 , pp. 569 - 582
Copyright
Copyright © The Nutrition Society 1983

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