Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-26T20:42:56.694Z Has data issue: false hasContentIssue false
  • English
  • Français

The responses of blood galactose to oral doses of lactose, galactose plus glucose and milk to piglets

Published online by Cambridge University Press:  09 March 2007

P. H. Bird ,
C. S. Atwood  and
P. E. Hartmann
P. H. Bird
Affiliation:
Department of Biochemistry, University of Western Australia, Nedlands WA 6009, Australia
C. S. Atwood
Affiliation:
Department of Biochemistry, University of Western Australia, Nedlands WA 6009, Australia
P. E. Hartmann
Affiliation:
Department of Biochemistry, University of Western Australia, Nedlands WA 6009, Australia
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The capacity of intestinal lactase (EC 3.2.1.23) of piglets to hydrolyse lactose in vivo was investigated by measuring the response of blood galactose to doses of lactose, galactose plus glucose and both whole and skimmed milk. Following the administration of oral doses of lactose dissolved in water to piglets from 2 to 18 d of age the adjusted galactose area under the curve (AUC) was between 1·12 and 1·36 arbitrary units, while following a dose of galactose plus glucose dissolved in water it was between 1·56 and 1·98 arbitrary units. Whereas these results suggest that the rate of digestion of lactose appeared to limit the amount of galactose reaching the peripheral blood after a dose of lactose dissolved in water, there was no significant correlation between the capacity of piglets to hydrolyse physiological amounts of lactose and the age of the piglets (2- to 18-d-old piglets; r 0·11). Following oral doses of sow's milk containing either lactose, or galactose plus glucose, the adjusted galactose AUC values were 0·94 and 1·00 arbitrary units respectively, in 10-d-old piglets. Thus, the limitation to the digestion of lactose observed when it was present in water was not evident for lactose in sow's milk. Since there was no significant difference between the adjusted galactose AUC following a dose of whole milk (0·95 arbitrary units) and that following a dose of skimmed milk (1·03 arbitrary units), the presence of fat in sow's milk did not appear to affect the utilization of lactose by the sucking piglets.

Keywords

PigletLactoseMilkBloodGalactose
Type
Lactose digestion in piglets
Information
British Journal of Nutrition , Volume 73 , Issue 5 , May 1995 , pp. 753 - 761
Copyright
Copyright © The Nutrition Society 1995

References

Arthur, P. G., Kent, J. C. & Hartmann, P. E. (1989 a). Milk lactose, citrate and glucose as markers of lactogenesis in normal and diabetic women. Journal of Pediatric Gastroenterology and Nutrition 9, 488–496.Google Scholar
Arthur, P. G., Kent, J. C. & Hartmann, P. E. (1989 b). Microanalysis of the metabolic intermediate of lactose synthesis in human milk and plasma using bioluminescent methods. Analytical Biochemistry 176, 449–456.CrossRefGoogle ScholarPubMed
Atwood, C. S. & Hartmann, P. E. (1992). Collection of fore and hind milk from the sow and the changes in milk composition during suckling. Journal of Dairy Research 59, 287–298.Google Scholar
Bailey, C. B., Kitts, W. D. & Wood, A. J. (1956). The development of the digestive enzyme system of the pig during its pre-weaning phase of growth. Canadian Journal of Agricultural Science 36, 51–59.Google Scholar
Bird, P. H. & Hartmann, P. E. (1994). The response in the blood of piglets to oral doses of galactose and glucose and intravenous administration of galactose. British Journal of Nutrition 71, 553–561.Google Scholar
Cooke, A. R. (1975). Control of gastric emptying and motility. Gastroenterology 68, 804–816.Google Scholar
Ekstrom, K. E., Benevenga, N. J. & Grummer, H. (1975). Changes in the intestinal lactase activity in the small intestine of two breeds of swine from birth to 6 weeks of age. Journal of Nutrition 105, 1032–1038.CrossRefGoogle ScholarPubMed
Fischer, J. E. & Sutton, T. S. (1949). Effects of lactose on gastro-intestinal motility: a review. Journal of Dairy Science 32, 139–162.CrossRefGoogle Scholar
Gregory, P. C., McFadyn, M. & Rayner, D. V. (1989). Control of gastric emptying in the pig: influence of duodenal infusions of glucose and emulsified fat. Quarterly Journal of Experimental Physiology 74, 109–119.Google Scholar
Hartman, P. A., Hays, V. W., Baker, R. O., Neagle, L. H. & Catron, D. V. (1961). Digestive enzyme development in the young pig. Journal of Animal Science 20, 114–123.Google Scholar
Hess, H. H., Lees, M. B. & Derr, J. E. (1978). A linear Lowry-Folin assay for both water-soluble and sodium dodecyl sulphate-solubilized proteins. Analytical Biochemistry 85, 295–300.CrossRefGoogle Scholar
James, P. S., Smith, M. W., Tivey, D. R. & Wilson, T. J. G. (1987). Epidermal growth factor selectively increases maltase and sucrase activities in neonatal piglet intestine. Journal of Physiology 393, 583–594.Google Scholar
Kulski, J. K. & Buehring, G. C. (1982). Microanalysis of lactose in tissue culture medium using an enzymatic-fluorometric method. Analytical Biochemistry 119, 341–350.Google Scholar
Kunst, A., Draeger, B. & Ziegenhorn, J. (1985). 2·4 D-glucose. 2·4·1. UV-methods with hexokinase and glucose-6-phosphate dehydrogenase. In Methods of Enzymatic Analysis, Vol. 6, pp. 163–172 [Bergmeyer, H. U., Bergmeyer, J. and Grabl, M., editors]. Weinheim: Verlag, Chemie.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.Google Scholar
Manners, M. J. & Stevens, J. A. (1972). Changes from birth to maturity in the pattern of distribution of lactase and sucrase activity in the mucosa of the small intestine of pigs. British Journal of Nutrition 28, 113–127.CrossRefGoogle ScholarPubMed
Nieft, M. L. & Deuel, H. J. (1947). The effect of fat on the absorption and utilisation of galactose by the rat. Journal of Biological Chemistry 167, 521–525.Google Scholar
Stern, I. & Shapiro, B. (1953). A rapid and simple method for the determination of esterified fatty acids and for total fatty acids in blood. Journal of Clinical Pathology 6, 158–160.Google Scholar
Walker, D. M. (1959). The development of the digestive system of the young animal. II. Carbohydrase enzyme development in the young pig. Journal of Agricultural Science 52, 357–363.Google Scholar
Widdowson, E. M., Colombo, V. E. & Artavanis, C. A. (1976). Changes in the organs of pigs in response to feeding for the first 24 hours after birth. II. The digestive tract. Biology of the Neonate 28, 272–281.Google Scholar
Williams, C. A., Philips, T. & Macdonald, I. (1983). The influence of glucose on serum galactose levels in man. Metabolism 32, 250–256.CrossRefGoogle ScholarPubMed
Woolridge, M. W. & Fisher, C. (1988). Colic, ‘overfeeding’, and symptoms of lactose malahsorption in the breast-fed baby: a possible artefact of feed management? Lancet 2, 382–385.Google Scholar
Yeh, K. C. & Kwan, K. C. (1978). A comparison of numerical integrating algorithms by trapezoidal, lagrange and spline approximation. Journal of Pharmacokinetics and Biopharmaceutics 6, 79–98.Google Scholar