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Relationship between the fatty acid composition of rat lymphocytes and immune functions

Published online by Cambridge University Press:  09 March 2007

Marguerite Moussa ,
Jean Tkaczuk ,
Jeannie Ragab ,
Jésus Garcia ,
Michel Abbal ,
Elie Ohayon ,
Jacques Ghisolfi  and
Jean-Paul Thouvenot
Marguerite Moussa*
Affiliation:
Group d'Etudes en Nutrition Infantile, Laboratoire de Biochimie, CHU Purpan, 31059 Toulouse Cedex, France
Jean Tkaczuk
Affiliation:
Immunologie CHU Rangueil, 31054 Toulouse Cedex, France
Jeannie Ragab
Affiliation:
Group d'Etudes en Nutrition Infantile, Laboratoire de Biochimie, CHU Purpan, 31059 Toulouse Cedex, France
Jésus Garcia
Affiliation:
Group d'Etudes en Nutrition Infantile, Laboratoire de Biochimie, CHU Purpan, 31059 Toulouse Cedex, France
Michel Abbal
Affiliation:
Immunologie CHU Rangueil, 31054 Toulouse Cedex, France
Elie Ohayon
Affiliation:
Immunologie CHU Rangueil, 31054 Toulouse Cedex, France
Jacques Ghisolfi
Affiliation:
Group d'Etudes en Nutrition Infantile, Laboratoire de Biochimie, CHU Purpan, 31059 Toulouse Cedex, France
Jean-Paul Thouvenot
Affiliation:
Group d'Etudes en Nutrition Infantile, Laboratoire de Biochimie, CHU Purpan, 31059 Toulouse Cedex, France
*
*Corresponding author: Dr Marguerite Moussa, fax +33 5 61 77 77 91, email [email protected]
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Abstract

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The effects of dietary lipids on the fatty acid composition, activation and proliferation of lymphocytes were investigated. Weanling male Wistar rats were fed for 8 weeks on one of two low-fat diets which contained 50 g lipid/kg, or one of two high-fat diets containing 200 g lipid/kg, from either coconut oil or soyabean oil. The fatty acid composition of phospholipids from splenocyte membranes was affected by dietary lipid manipulation, and these differences influenced lymphocyte functions. Increased levels of linoleic acid in spleen lymphocytes correlated negatively with interleukin-2 receptor α-chain expression determined either by measuring the mean fluorescence or by the proportion of cells staining positive for CD25, and with the cell proliferation index. However, we found a positive correlation between interleukin-2 receptor α-chain expression determined by measuring the mean fluorescence and the cell proliferation index with the oleic acid concentration of spleen lymphocytes. Since phospholipid hydrolysis occurs early in lymphocyte activation, immunosuppressive effects induced by polyunsaturated fatty acids, described in the literature, could be due to an increase of linoleic acid or a decrease of oleic acid affecting many components of plasma-membrane-associated events involved in lymphocyte activation.

Keywords

Lymphocyte proliferationOleic acidLinoleic acidInterleukin-2 receptor
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 3 , March 2000 , pp. 327 - 333
Copyright
Copyright © The Nutrition Society 2000

References

Berger, A, German, JB and Chiang, BL (1993) Influence of feeding unsaturated fats on growth and immune status of mice. Journal of Nutrition 123, 225–233.Google ScholarPubMed
Calder, PC (1998) Dietary fatty acids and immune system. Nutrition Reviews 56, 570–583.Google Scholar
Calder, PC, Bevan, SJ and Newsholme, EA (1992) The inhibition of T-lymphocyte proliferation by fatty acids is via an eicosanoid independent mechanism. Immunology 75, 108–115.Google Scholar
Calder, PC, Bond, JA, Bevan, SJ, Hunt, SV and Newsholme, EA (1991) Effect of fatty acids on the proliferation of concanavalin A-stimulated rat lymph node lymphocyte. International Journal of Biochemistry 23, 579–588.CrossRefGoogle Scholar
Calder, PC, Costarosa, LFBP and Curi, R (1995) Effects of feeding lipids of different fatty acid compositions upon rat lymphocyte proliferation. Life Sciences 56, 455–463.CrossRefGoogle ScholarPubMed
Calder, PC and Newsholme, EA (1992) Polyunsaturated fatty acids suppress human peripheral blood lymphocyte proliferation and interleukin-2 production. Clinical Science 82, 695–700.CrossRefGoogle ScholarPubMed
Calder, PC and Newsholme, EA (1992) Unsaturated fatty acids suppress interleukin-2 production and transferrin receptor expression by concanavalin A stimulated rat lymphocytes. Mediators of Inflammation 1, 107–112.CrossRefGoogle Scholar
Calder, PC, Yaqoob, P, Harvey, DJ, Watts, A and Newsholme, EA (1994) Incorporation of fatty acids by concanavalin A stimulated lymphocytes and the effect on fatty acid composition and membrane fluidity. Biochemical Journal 300, 509–518.CrossRefGoogle ScholarPubMed
Erickson, KL, Adams, DA and McNeill CJ (1983) Dietary lipid modulation of immune responsiveness. Lipids 18, 468–474.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M and Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497–509.Google Scholar
Fowler, KH, McMurray, DN, Fan, YY, Aukema, HM and Chapkin, RS (1993) Purified dietary. n-3 polyunsaturated fatty acids alter diacylglycerol mass and molecular species composition in concanavalin A-stimulated murine splenocytes. Biochimica et Biophysica Acta 1210, 89–96.Google Scholar
Fra, AM, Williamson, E, Simons, K and Parton, RG (1994) Detergent-insoluble glycolipid microdomains in lymphocytes in the absence of caveolae. Journal of Biological Chemistry 269, 30745–30748.CrossRefGoogle ScholarPubMed
Garcia, J, Ghisolfi, J, Lapalu-Traon C, Periquet, B, Olives, JP, Boyer, MJ and Thouvenot, JP (1986) Depistage d'une carence en acides gras essentiels chez l'enfant (Screening for essential fatty acid deficiency in the infant). Annales de Biologie Clinique 44, 380–383.Google Scholar
Gogos, CA and Kalfarentzos, F (1995) Total parenteral nutrition and immune system activity: a review. Nutrition 11, 339–344.Google ScholarPubMed
Guillou, PJ (1993) The effects of lipids on some aspects of the cellular immune response. Proceedings of the Nutrition Society 52, 91–100.CrossRefGoogle ScholarPubMed
Holman, RT (editor) (1968) Essential Fatty Acid Deficiency. Oxford: Pergamon Press.Google Scholar
Innis, SM (1991) Essential fatty acids in growth and development. Progress in Lipid Research 30, 39–103.Google Scholar
Jeffery, NM, Yaqoob, P, Newsholme, EA and Calder, PC (1996) The effects of olive oil upon rat serum lipid levels and lymphocyte function appear to be due to oleic acid. Annals of Nutrition and Metabolism 40, 71–80.Google Scholar
Levy, JA, Ibrahim, AB, Shirai, I, Okta, K, Nagasawa, R, Yoshida, H, Estes, J and Gardner, M (1982) Dietary fat affects immune response, production of antiviral factors and immune complex disease in NZB/NZW mice. Proceedings of the National Academy of Sciences USA 79, 1974–1978.Google Scholar
Lockniskar, M, Nauss, KM and Newberne, PM (1983) The effect of quality and quantity of dietary fat on the immune system. Journal of Nutrition 113, 951–961.Google Scholar
Meydani, SN, Endres, S, Woods, MM, Golden, BR, Soo, C, Morrill-Labrode A, Dinarello, CA and Gorbach, SL (1991) Oral (. n-3) fatty acid supplementation suppresses cytokine production and lymphocyte proliferation: comparison between young and older women. Journal of Nutrition 121, 547–555.Google Scholar
Mossman, T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65, 55–63.Google Scholar
Moussa, M, Garcia, J, Ghisolfi, J, Periquet, B and Thouvenot, JP (1996) Dietary essential fatty acid deficiency differentially affects tissues of rats. Journal of Nutrition 126, 3040–3045.Google Scholar
Ossman, JB, Erickson, KL and Canolty, NL (1980) Effects of saturation and concentration of dietary fats on lymphocyte transformation in mice. Nutrition Reports International 22, 279.Google Scholar
Palmblad, J (1991) Intravenous lipid emulsions and host defence — a critical review. Clinical Nutrition 10, 303–308.CrossRefGoogle ScholarPubMed
Potier de Courcy, G, Durand, G, Abraham, J and Gueguen, L (1989) Recommandations sur les conditions d'alimentation des animaux de laboratoire (rats et souris) (Recommendations on feeding conditions for laboratory animals (rats and mice)). Science des Aliments 9, 209–217.Google Scholar
Sanderson, P, Yaqoob, P and Calder, PC (1985) Effects of dietary lipid manipulation upon rat spleen lymphocyte functions and the expression of lymphocyte surface molecules. Journal of Nutritional and Environmental Medicine 5, 119–132.CrossRefGoogle Scholar
Soyland, E, Nenseter, MS, Braathen, L and Drevon, CA (1993) Very long chain. n-3 and. n-6 polyunsaturated fatty acids inhibit proliferation of human T-lymphocytes in vitro. European Journal of Clinical Investigation 23, 112–121.CrossRefGoogle ScholarPubMed
Tsang, WM, Weyman, C and Smith, AD (1977) Effect of fatty acid mixtures on phytohaemagglutinin-stimulated lymphocytes from different species. Biochemical Society Transactions 5, 153–159.Google Scholar
Weiss, A, Shield, R, Newton, M, Manger, B and Imboden, J (1987) Ligand receptor inter-actions required for commitment to the activation of the interleukin 2 gene. Journal of Immunology 138, 2169–2176.Google Scholar
Yaqoob, P, Newsholme, EA and Calder, PC (1994) The effect of dietary lipid manipulation on rat lymphocyte subsets and proliferation. Immunology 82, 603–610.Google Scholar