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Rabbits fed on β-carotene have higher serum levels of all-trans retinoic acid than those receiving no β-carotene

Published online by Cambridge University Press:  09 March 2007

Y. Folman ,
R. M. Russell ,
G. W. Tang  and
G. Wolf
Y. Folman
Affiliation:
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139
R. M. Russell
Affiliation:
USDA-Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA
G. W. Tang
Affiliation:
USDA-Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA
G. Wolf
Affiliation:
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139
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Abstract

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The aim of the present work was to determine the effect of giving increasing doses of supplements of β-carotene on serum retinoic acid levels in rabbits. Four groups of 7-week-old female rabbits were fed for 9 weeks on a pelleted diet containing 1.72 mg vitamin A as retinyl acetate/kg and including control gelatin beadlets devoid of β-carotene or 1, 2 or 4 mg β-carotene/kg body-weight per d. Serum was collected at 3, 6 and 9 weeks after the beginning of the experiment and the concentration of all-trans retinoic acid was determined by a gradient reverse-phase high-performance liquid chromatography system following a double-phase extraction. The average concentration of retinoic acid in serum of the combined control and 1 mg β-carotene/kg groups was 3.80, 3.06 and 2.40 nM at 3, 6 and 9 weeks respectively. The concentrations of retinoic acid in serum of the combined 2 and 4 mg β-carotene/kg groups were 4.80 nM (P < 0.05), 3.76 nM (not significant) and 4.90 nM (P < 0.005) at 3, 6 and 9 weeks respectively. A SAS (SAS Institute Inc., 1985) general linear model repeated-measures analysis of variance revealed that the effects of treatment (P <0.01), time (P < 0.05) and treatment x time interaction (P < 0.05) were statistically significant. It is concluded that giving β-carotene is associated with higher concentrations of all-trans retinoic acid in the serum of rabbits than in those receiving no β-carotene.

Keywords

β-CaroteneRetinoic acidRabbit
Type
Research Article
Information
British Journal of Nutrition , Volume 62 , Issue 1 , July 1989 , pp. 195 - 201
Copyright
Copyright © The Nutrition Society 1989

References

REFERENCES

Bankson, D.D., Russell, R.M. & Sadowski, J.A. (1986). Determination of retinyl esters and retinol in serum and plasma by normal phase chromatography: methods and applications. Clinical Chemistry 32, 35–40.CrossRefGoogle Scholar
Barua, A.B. & Olson, J.A. (1986). Retinyl β-glucuronide, an endogenous compound of human blood. American Journal of Clinical Nutrition 43, 481–485.CrossRefGoogle Scholar
Crain, F.D., Lotspeich, F.J. & Krause, R.F. (1967). Biosynthesis of retinoic acid by intestinal enzymes of the rat. Journal of Lipid Research 8, 249–254.CrossRefGoogle ScholarPubMed
Cullum, M.E. & Zile, M.H. (1985). Metabolism of all-trans-retinoic acid and all-trans retinyl acetate. Journal of Biological Chemistry 260, 10590–10596.CrossRefGoogle ScholarPubMed
De Leenheer, A.P., Lambert, W.E. & Claeys, I. (1982). All-trans retinoic acid: measurement of reference values in human serum by high performance liquid chromatography. Journal of Lipid Research 23, 1362–1367.CrossRefGoogle ScholarPubMed
De Ruyter, M.G., Lambert, W.E. & De Leenheer, A.P. (1979). Retinoic acid: an endogenous compound of human blood. Analytical Biochemistry 98, 402–409.CrossRefGoogle ScholarPubMed
Dowling, J.E. & Wald, G. (1960). The biological function of vitamin A acid. Proceedings of the National Academy of Sciences, USA 46, 587–608.CrossRefGoogle ScholarPubMed
Dunagin, P.E., Zachman, R.D. & Olson, J.A. (1964). Identification of free and conjugated retinoic acid as a product of retinal (vitamin A aldehyde) metabolism in the rat in vivo. Biochimica et Biophysica Acta 90, 432–434.CrossRefGoogle ScholarPubMed
Emerick, R.J., Zile, M. & De Luca, H.F. (1967). Formation of retinoic acid from retinol in the rat. Biochemical Journal 102, 606–611.CrossRefGoogle ScholarPubMed
Folman, Y., Ascarelli, I., Kraus, D. & Barash, H. (1987). Adverse effect of β-carotene on fertility of dairy cows. Journal of Dairy Science 70, 357–366.CrossRefGoogle ScholarPubMed
Folman, Y., Rosenberg, M., Ascarelli, I., Kaim, M. & Herz, Z. (1983). The effect of dietary and climatic factors on fertility and on plasma progesterone and oestradiol-17 β-levels in dairy cows. Journal of Steroid Biochemistry 19, 863–868.CrossRefGoogle ScholarPubMed
Frolik, C.A. (1984). Metabolism of retinoids. In The Retinoids, vol. 2, pp. 177–208 [Sporn, M.B., Roberts, A.B. and Goodman, D.S., editors]. Orlando: Academic Press.Google Scholar
Frolik, C.A., Dart, L.L. & Sporn, M.B. (1981). Metabolism of all-trans retinyl acetate to retinoic acid in hamster tracheal organ culture. Biochemica et Biophysica Acta 663, 329–335.CrossRefGoogle ScholarPubMed
Greenberg, E.R., Baron, J.A. & Beck, J.R. (1985). Carotenoids and cancer prevention. In Retinoids: New Trends in Research and Therapy, pp. 360–370 [Saurat, J., editor]. Basel: S. Karger.Google Scholar
Jurkowitz, L. (1962). Determination of vitamin A in human plasma after oral administration. Archives of Biochemistry and Biophysics 98, 337–341.CrossRefGoogle ScholarPubMed
Kemmann, E., Pasquale, S.A. & Skaf, R. (1983). Amenorrhea associated with carotenemia. Journal of the American Medical Association 249, 926–929.CrossRefGoogle ScholarPubMed
Kleiner-Bossaler, A. & De Luca, H.F. (1971). Formation of retinoic acid from retinol in the kidney. Archives of Biochemistry and Biophysics 142, 371–377.CrossRefGoogle ScholarPubMed
Lambert, W.E. & De Leenheer, A.P. (1985). Demonstration of retinoic acid isomers in human urine under physiological conditions. Experientia 41, 359–360.CrossRefGoogle ScholarPubMed
Mahadevan, S., Murthy, S.K. & Ganguly, J. (1962). Enzymic oxidation of vitamin A aldehyde to vitamin A acid by rat liver. Biochemical Journal 85, 326–331.CrossRefGoogle ScholarPubMed
Napoli, J.L., Pramanik, B.C., Williams, J.B., Dawson, M.I. & Hobbs, P.D. (1985). Quantification of retinoic acid by gas-liquid chromatography-mass spectrometry: total versus all-trans-retinoic acid in human plasma. Journal of Lipid Research 26, 387–392.CrossRefGoogle ScholarPubMed
Napoli, J.L. & Race, K.R. (1988). Biogenesis of retinoic acid from β-carotene. Journal of Biological Chemistry 263, 17372–17377.CrossRefGoogle ScholarPubMed
Ong, D.E., Crow, J.A. & Chytil, F. (1982). Radioimmunochemical determination of cellular retinol and cellular retinoic acid-binding proteins in cytosol of rat tissue. Journal of Biological Chemistry 257, 13385–13389.CrossRefGoogle Scholar
Ott, D.B. & Lachance, P.A. (1979). Retinoic acid–a review. American Journal of Clinical Nutrition 32, 2522–2531.CrossRefGoogle ScholarPubMed
Roberts, A.B. & Sporn, M.B. (1984). Cellular biology and biochemistry of the retinoids. In The Retinoids, vol. 2, pp. 209–286 [Sporn, M.B., Roberts, A.B. and Goodman, D.S., editors]. Orlando: Academic Press.Google Scholar
SAS Institute Inc. (1985). SAS User's Guide: Statistics, version 5, pp. 478–483. Cary, North Carolina: SAS Institute Press.Google Scholar
Shapiro, S.S., Mott, D.J. & Machlin, L.J. (1984). Kinetic characteristics of β-carotene uptake and depletion in rat tissue. Journal of Nutrition 114, 1924–1933.CrossRefGoogle ScholarPubMed
Thompson, J.N., Howell, J. McC., Pitt, G.A.J. & McLaughlin, C.I. (1969). The biological activity of retinoic acid in the domestic fowl and the effects of vitamin A deficiency on the chick embryo. British Journal of Nutrition 23, 471–490.CrossRefGoogle ScholarPubMed
Williams, D.A. (1972). The comparison of several dose levels with a zero dose control. Biometrics 28, 519–531.CrossRefGoogle ScholarPubMed
Wolf, G. (1984). Multiple functions of vitamin A. Physiological Reviews 64, 873–937.CrossRefGoogle ScholarPubMed
Zile, M.H. & Cullum, M.E. (1983). The function of vitamin A: current concepts. Proceedings of the Society for Experimental Biology and Medicine 172, 139–152.CrossRefGoogle ScholarPubMed