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Protein v. enzymic protein hydrolysates. Nitrogen utilization in starved rats

Published online by Cambridge University Press:  09 March 2007

Julio J. Boza ,
Olga Martínez-Augustin ,
Luis Baró ,
M. Dolores Suarez  and
Angel Gil
Julio J. Boza
Affiliation:
Department of Biochemistry and Molecular Biology, University of Granada, Spain
Olga Martínez-Augustin
Affiliation:
PULEVA Research and Development, Granada, Spain
Luis Baró
Affiliation:
PULEVA Research and Development, Granada, Spain
M. Dolores Suarez
Affiliation:
Department of Biochemistry and Molecular Biology, University of Granada, Spain
Angel Gil
Affiliation:
PULEVA Research and Development, Granada, Spain
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Abstract

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The present study was carried out to compare the effects of four isoenergetic and isonitrogenous diets on the N utilization, total serum protein concentration and serum amino acid profile in starved rats at weaning. These diets differed only in the molecular form of two milk proteins (whey protein and casein), which were either native or partly hydrolysed. Male Wistar rats at weaning were fasted for 3 d and then refed with one of the four diets for 48 h. No differences were observed in the body weight gain, protein digestibility and total serum protein concentration between groups after the refeeding period and all the N balances were positive. N retention was higher in the two groups of rats given the protein-hydrolysate-based diets compared with those given the intact-protein-based diets. This was associated with a lower urinary N excretion in rats, given the whey-protein-hydrolysate and the casein-hydrolysate diets. Despite this fact, the serum amino acid pattern of rats given the hydrolysed protein diet was very similar to that of those given the corresponding native protein diet. In conclusion, we have proved that enzymic hydrolysates from milk proteins have equivalent effects to native proteins in recovery after starvation in rats at weaning, on N absorption, total serum protein concentration and serum amino acid profile, and even give a higher N retention. We did not observe any harmful effect in using protein hydrolysates instead of native proteins.

Keywords

ProteinNitrogenStarvation
Type
Digesta kinetics and feed intake in silage-fed animals
Information
British Journal of Nutrition , Volume 73 , Issue 1 , January 1995 , pp. 65 - 71
Copyright
Copyright © The Nutrition Society 1995

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