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Protein synthesis in vivo in rats fed on lipid-rich liquid diets

Published online by Cambridge University Press:  09 March 2007

Ernesto Estornell ,
Teresa Barber  and
José Cabo
Ernesto Estornell
Affiliation:
Departament de Bioquímica i Biologia Molecular, Universitat de Valéncia. Facultat de Farmàcia, Avgda. Vicent Andrés Estellés s/n, 46100-Burjassot (Valéncia), Spain.
Teresa Barber
Affiliation:
Departament de Bioquímica i Biologia Molecular, Universitat de Valéncia. Facultat de Farmàcia, Avgda. Vicent Andrés Estellés s/n, 46100-Burjassot (Valéncia), Spain.
José Cabo
Affiliation:
Departament de Bioquímica i Biologia Molecular, Universitat de Valéncia. Facultat de Medicina i Odontologia, Avgda. Vicent Andrés Estellés s/n, 46100-Burjassot (Valéncia), Spain.
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Abstract

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Changes in tissue composition and protein synthesis ratio were studied in the major tissues of the body in young rats fed on lipid-rich, isonitrogenous purified liquid diets, a convenient method for inducing voluntary overfeeding under controlled nutritional conditions. Overfed rats showed faster growth induced by the energy excess. Analysis of tissue composition (protein, DNA and RNA contents) revealed that growth was due mainly to tissue hyperplasia in which protein and DNA contents increased in parallel. Fractional protein synthesis ratio measured in vivo by the flooding-dose method of phenylalanine showed a marked increase in all tissues. This change could be attributed to an increase in the ribosomal activity for protein synthesis in most tissues. Therefore, our results indicate that addition of a supplementary energy source (as lipids) to a well-balanced diet improves growth and protein synthesis in growing rats.

Keywords

OverfeedingTissue compositionProtein synthesis
Type
Protein synthesis on low-fat diets
Information
British Journal of Nutrition , Volume 72 , Issue 4 , October 1994 , pp. 509 - 517
Copyright
Copyright © The Nutrition Society 1994

References

REFERENCES

American Institute of Nutrition (1977). Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
American Institute of Nutrition (1980). Second report of the ad hoc committee on standards for nutritional studies. Journal of Nutrition 110, 1726.CrossRefGoogle Scholar
Barber, T., Estornell, E., Estelle´s, R., Go´mez, D. & Cabo, J. (1987). Studies on the role of insulin in N metabolism changes in cafeteria-fed rats. Molecular and Cellular Endocrinology 50, 15–22.CrossRefGoogle Scholar
Barber, T., Garcia de la Asuncio´n, J., Puertes, I. R. & Vin´a, J. R. (1990). Amino acid metabolism and protein synthesis in lactating rats fed on a liquid diet. Biochemical Journal 270, 77–82.Google Scholar
Barber, T., Vina, J. R., Vina, J. & Cabo, J. (1985). Decreased urea synthesis in cafeteria-diet-induced obesity in the rat. Biochemical Journal 230, 675–681.CrossRefGoogle ScholarPubMed
Estornell, E., Barber, T. & Cabo, J. (1994). Improved nitrogen metabolism in rats fed on lipid-rich liquid diets. British Journal of Nutrition 71, 361–373.CrossRefGoogle ScholarPubMed
Garlick, P. J., Fern, E. B., McNurlan, M. A. & Preedy, V. R. (1983). The effect of insulin infusion and food intake on muscle protein synthesis in postabsorptive rats. Biochemical Journal 210, 669–676.CrossRefGoogle ScholarPubMed
Garlick, P. J., McNurlan, M. A. & Preedy, V. R. (1980). A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine. Biochemical Journal 192, 719–723.CrossRefGoogle ScholarPubMed
Glick, Z., McNurlan, M. A. & Garlick, P. J. (1982). Protein synthesis rate in liver and muscle of rats following four days of overfeeding. Journal of Nutrition 112, 391–397.Google Scholar
Goldspink, D. F. & Kelly, F. J. (1984). Protein turnover and growth in the whole body, liver and kidney of the rat from the foetus to senility. Biochemical Journal 217, 507–516.CrossRefGoogle ScholarPubMed
Jansen, G. R. & Hunsaker, H. (1986). Effect of dietary protein and energy on protein synthesis during lactation in rats. Journal of Nutrition 116, 957–968.CrossRefGoogle ScholarPubMed
Kinney, J. M. & Elwyn, D. H. (1983). Protein metabolism and injury. Annual Review of Nutrition 3, 433–466.CrossRefGoogle ScholarPubMed
Kita, K., Muramatsu, T., Tasaki, I. & Okumura, J. (1989). Influence of dietary non-protein energy intake on whole-body protein turnover in chicks. British Journal of Nutrition 61, 235–244.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle Scholar
McNurlan, M. A., Fern, E. B. & Garlick, P. J. (1982). Failure of leucine to stimulate protein synthesis in vivo. Biochemical Journal 204, 831–838.CrossRefGoogle ScholarPubMed
McNurlan, M. A., Tomkins, A. M. & Garlick, P. J. (1979).The effect of starvation on the rate of protein synthesis in rat liver and small intestine. Biochemical Journal 178, 373–379.CrossRefGoogle Scholar
Minari, O. & Zilversmit, D. B. (1963). Use of KCN for stabilization of color in direct Nesslerization of Kjeldahl digest. Analytical Biochemistry 6, 320–327.Google Scholar
Munro, H. N. (1951). Carbohydrate and fat as factors in protein utilization and metabolism. Physiological Reviews 31,449–488.CrossRefGoogle ScholarPubMed
Munro, H. N. (1964). General aspects of the regulation of protein metabolism by diet and by hormones. In Mammalian Protein Metabolism, Vol. 1, pp. 381–481 [Munro, H. N. and Allison, J. B., editors]. New York: Academic Press.Google Scholar
Munro, H. N. (1978). Energy and protein intakes as determinants of nitrogen balance. Kidney International 14, 313–316.CrossRefGoogle ScholarPubMed
Munro, H. N. & Fleck, A. (1969). Analysis of tissues and body fluids for nitrogenous constituents. In Mammalian Protein Metabolism, Vol. 3, pp. 423–525 [Munro, H. N., editor]. New York: Academic Press.CrossRefGoogle Scholar
Muramatsu, T. & Okumura, J. I. (1985). Whole-body protein turnover in chicks at early stages of growth. Journal of Nutrition 115, 483–490.CrossRefGoogle ScholarPubMed
Muramatsu, T., Takai, D., Kita, K. & Okumura, J. I. (1990). Studies on the measurement of whole-body protein degradation In vivo in the chicken. International Journal of Biochemistry 22, 153–158.Google ScholarPubMed
National Research Council (1978). Nutrient requirements of laboratory animals. In Nutrient Requirements of Domestic Animals, no. 10, 3rd ed., pp. 7–37. Washington D.C: National Academy of Sciences.Google Scholar
Odedra, B. R., Bates, P. C. & Millward, D. J. (1983). Time course of the effect of catabolic doses of corticosterone on protein turnover in rat skeletal muscle and liver. Biochemical Journal 214, 617–627.CrossRefGoogle ScholarPubMed
Reeds, P. J., Cadenhead, A., Fuller, M. F., Lobley, G. E. & McDonald, J. D. (1980). Protein turnover in growing pigs. Effects of age and food intake. British Journal of Nutrition 43, 445–455.CrossRefGoogle ScholarPubMed
Reeds, P. J., Fuller, M. F., Cadenhead, A., Lobley, G. E. & McDonald, J. D. (1981). Effects of changes in the intakes of protein and non-protein energy on whole-body protein turnover in growing pigs. British Journal of Nutrition 45, 539–546.CrossRefGoogle ScholarPubMed
Reeds, P. J. & James, W. P. T. (1983). Protein turnover. Lancet 1, 571–574.CrossRefGoogle ScholarPubMed
Sampson, D. A., Masor, M. & Jansen, G. R. (1984). Protein synthesis in rat tissue during lactation: no effect of diethyl ether anaesthesia. Biochemical Journal 234, 681–683.CrossRefGoogle Scholar
Waterlow, J. C. (1986). Metabolic adaptation to low intakes of energy and protein. Annual Review of Nutrition 6, 495–526.Google Scholar
Waterlow, J. C., Garlick, P. J. & Millward, D. J. (1978). Protein Turnover in Mammalian Tissues and in the Whole Body. Amsterdam: North-Holland.Google Scholar
Waterlow, J. C. & Jackson, A. A. (1981). Nutrition and protein turnover in man. British Medical Bulletin 37, 5–10.CrossRefGoogle ScholarPubMed