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Protein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolism

Published online by Cambridge University Press:  08 March 2007

Álvaro Belenguer ,
Joaquim Balcells ,
Jose A. Guada ,
Marc Decoux  and
Eric Milne
Álvaro Belenguer
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Miguel Servet 177, Zaragoza 50013, Spain
Joaquim Balcells*
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Miguel Servet 177, Zaragoza 50013, Spain
Jose A. Guada
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Miguel Servet 177, Zaragoza 50013, Spain
Marc Decoux
Affiliation:
Cargill, Paseig Sant Joan 193, Barcelona, Spain
Eric Milne
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK
*
*Corresponding author: Dr Joaquim Balcells, fax +34 976 761590, email [email protected]
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Abstract

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To study the absorption of microbial lysine in growing rabbits, a labelled diet (supplemented with 15NH4Cl) was administered to six animals (group ISOT); a control group (CTRL, four rabbits) received a similar, but unlabelled, diet. Diets were administered for 30d. An additional group of six animals were fed the unlabelled diet for 20d and then the labelled diet for 10d while wearing a neck collar to avoid caecotrophy (group COLL), in order to discriminate it from direct intestinal absorption. At day 30 animals were slaughtered and caecal bacteria and liver samples taken. The 15N enrichment in amino acids of caecal bacteria and liver were determined by GC–combustion/isotope ratio MS. Lysine showed a higher enrichment in caecal microflora (0·925 atom% excess, APE) than liver (0·215 APE) in group ISOT animals, confirming the double origin of body lysine: microbial and dietary. The COLL group showed a much lower enrichment in tissue lysine (0·007 (se 0·0029) APE for liver). Any enrichment in the latter animals was due to direct absorption of microbial lysine along the digestive tract, since recycling of microbial protein (caecotrophy) was avoided. In such conditions liver enrichment was low, indicating a small direct intestinal absorption. From the ratio of [15N]lysine enrichment between liver and bacteria the contribution of microbes to body lysine was estimated at 23%, with 97% of this arising through caecotrophy. Absorption of microbial lysine through caecotrophy was 119 (se 4·0) mg/d, compared with 406 (se 1·8) mg/d available from the diet. This study confirms the importance of caecotrophy in rabbit nutrition (15% of total protein intake).

Keywords

RabbitCaecotrophyMicrobial lysine15N kinetics
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 5 , November 2005 , pp. 763 - 770
Copyright
Copyright © The Nutrition Society 2005

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