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Protein expression of pectoralis major muscle in chickens in response to dietary methionine status

Published online by Cambridge University Press:  08 March 2007

A. Corzo ,
M. T. Kidd ,
W. A. Dozier III ,
L. A. Shack  and
S. C. Burgess
A. Corzo*
Affiliation:
Department of Poultry Science, Mississippi State University, MS 39762, USA
M. T. Kidd
Affiliation:
Department of Poultry Science, Mississippi State University, MS 39762, USA
W. A. Dozier III
Affiliation:
USDA-ARS Poultry Research Unit, Mississippi State, MS 39762, USA
L. A. Shack
Affiliation:
Department of Basic Sciences, Mississippi State University, MS 39762, USA
S. C. Burgess
Affiliation:
Department of Basic Sciences, Mississippi State University, MS 39762, USA
*
*Corresponding author: Dr Alejandro Corzo, fax +1 662 325 8292, email [email protected]
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Abstract

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The present study evaluated the effect of dietary methionine on breast-meat accretion and protein expression in skeletal muscle of broiler chickens in vivo. All broilers received a common pre-test diet up to 21d of age, and were subsequently fed either a methionine-deficient (MD) or -adequate (MA) diet (3·1 V. 4·5g/kg diet) from age 21 to 42d. Dietary cystine levels were 3·7 V. 3·6g/kg diet for the MD and MA diet, respectively. Detrimental effects on carcass yield (P=0·004), abdominal fat percentage (P=0·001), and breast-meat weight (P=0·001), yield (P=0·001), and uniformity (P=0·002) were observed and validated in birds fed MD diets. Via tandem MS, a total of 190 individual proteins were identified from pectoralis major (PM) muscle tissue. From the former composite, peptides from three proteins were observed to be present exclusively in breast muscle from those chickens fed the MD diet (pyruvate kinase, myosin alkali light chain-1, ribosomal-protein-L-29). No proteins were observed to be uniquely expressed in chickens fed MA diets. Research is warranted to further explore the possibility of the proteins pyruate kinase, myosin alkali light chain-1, or ribosomal protein L-29, as potential biological indicators of differences in protein expression of PM of chickens in response to a dietary methionine deficiency.

Keywords

Mass spectrometryMethionineProtein expressionProteomics
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 4 , April 2006 , pp. 703 - 708
Copyright
Copyright © The Nutrition Society 2006

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