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Profiles of faecal output of rare earth elements and stable isotopic tracers of iron and zinc after oral administration

Published online by Cambridge University Press:  09 March 2007

Ulvi Ulusoy*
Affiliation:
Cumhuriyet University, Department of Chemistry, 58140-Sivas, Turkey
John E. Whitley
Affiliation:
Scottish Universities Research and Reactor Centre, Rankine Avenue, Scottish Enterprise Technology Park, East Kilbride G75 0QF, UK
*
*Corresponding author: Dr Ulvi Ulusoy, fax +90 346 2191186, email [email protected]
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Abstract

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The objectives of this study were to confirm the non-absorbability and the reproducibility of faecal excretion kinetics of orally administered rare earth elements, and to investigate the excretion profiles of rare earth elements and stable isotopic tracers of Fe and Zn to establish the extent to which rare earth element markers duplicate the behaviour of isotopic tracers. Two investigations were performed: (1) six healthy subjects consumed a solution containing five rare earth elements in amounts varying from 1 to 10 mg; (2) seven healthy subjects were given a standard solution labelled with Sm marker and 57Fe tracer, and a meal labelled with Yb marker and 58Fe and 70Zn tracers. Individual faecal samples were collected and analysed to determine recoveries of rare earth elements and unabsorbed isotopic tracers. The mean values for recoveries were 94·1 (SD 4·5) % FOR THE FIVE RARE EARTH ELEMENTS, AND 103 (sd 3·0) % and 99·8 (sd 2·8) % for Sm and Yb respectively. For Fe consumed with the solution, excretion kinetics of the rare earth element marker and unabsorbed tracers with cumulative collections of the first two and three faecal samples were identical, but endogenous excretion of Fe was significant (P<0·05) in stools collected after the third. For Fe and Zn consumed with the meal, the excretion kinetics for the first two individual faecal samples and composites of sequential outputs were identical. Rare earth elements can be used as markers in studies of measurement of absorption. The dose of tracer required for the measurement of absorption would be reduced proportionally to the reduction of the period of faecal sampling, so that studies with stable isotopes would be more economical, thus enabling epidemiological investigations.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2000

References

Allen, LH, Raynolds, WL and Margen, S (1979) Polyethylene glycol as a quantitative faecal marker in human nutrition experiments. American Journal of Clinical Nutrition 32, 427440.Google Scholar
Björn-Rasmussen, E, Hallberg, L and Walker, RB (1972) Food iron absorption in man. I. Isotopic exchange between food iron and inorganic iron salt added to food: studies on maize, wheat, and eggs. American Journal of Clinical Nutrition 25, 317323.Google Scholar
Charlton, RW and Bothwell, TH (1983) Iron absorption. Annual Review of Medicine 34, 5568.CrossRefGoogle ScholarPubMed
Cook, JD, Dassenko, SA and Lynch, SR (1991) Assessment of the role of nonheme-iron availability in iron balance. American Journal of Clinical Nutrition 54, 717722.Google Scholar
Crock, JG and Lichte, FE (1982) Determination of rare earth elements in geological materials by inductively coupled argon plasma/atomic emission spectrometry. Analytical Chemistry 54, 13291332.Google Scholar
Cummings, JH and Wiggins, HS (1976) Transit through the gut measured by analysis of a single stool. Gut 17, 219223.CrossRefGoogle ScholarPubMed
Esposito, M, Collecchi, P, Martines, H, Aste, H, Merlo, F, Oddone, M, Meloni, S, Caramella Crespi, V and Genova, N (1993) Plasma and tissue levels of some lanthanide elements in human colorectal lesions. Journal of Radioanalytical and Nuclear Chemistry 174, 2328.CrossRefGoogle Scholar
Fairweather-Tait, SJ, Fox, TE, Wharf, SG, Eagles, JI and Kennedy, H (1992) Zinc absorption in adult men from chicken sandwich made with white or wholemeal bread, measured by a double-label stable isotope technique. British Journal of Nutrition 67, 411419.Google Scholar
Fairweather-Tait, SJ, Jackson, MJ, Fox, TE, Wharf, SG, Eagles, J and Croghan, PC (1993) The measurement of exchangeable pools of zinc using the stable isotope 70Zn. British Journal of Nutrition 70, 221234.CrossRefGoogle ScholarPubMed
Fairweather-Tait, SJ, Minihane, AM, Eagles, J, Owen, L and Helen, MC (1997) Rare earth elements as nonabsorbable fecal markers in studies of iron absorption. American Journal of Clinical Nutrition 65, 970976.CrossRefGoogle ScholarPubMed
Flanagan, PR, Cluett, J, Chamberline, MJ and Valberg, LS (1985) Dual isotope method for determination of human zinc absorption: the use of a test meal of turkey meat. Journal of Nutrition 115, 111122.CrossRefGoogle ScholarPubMed
Forbes, AL, Adams, CE, Arnaud, MJ, Chicester, CO, Cook, JD, Harrison, BN, Hurrell, RF, Kahn, SG, Morris, ER, Tanner, JT and Whittaker, P (1989) Comparison of in vitro, animal, and clinical determinations of iron bioavailability: International Nutritional Anemia Consultative Group Task Force report on iron bioavailability. American Journal of Clinical Nutrition 49, 225238.Google Scholar
Hasegawa, Y, Sugawara, T and Choppin, GR (1988) Thermodynamic parameters of complexation of lanthanoid(III) with ascorbic acid. Inorganic Chimica Acta 143, 277280.Google Scholar
Hinton, JM, Lennard-Jones, JE and Young, AC (1969) A new method for studying gut transit times using radiopaque markers. Gut 10, 842847.Google Scholar
Hutcheson, DP, Venugopal, B, Gray, DH and Luckey, TD (1975) Studies of nutritional safety of some heavy metals in mice. Journal of Nutrition 105, 670675.CrossRefGoogle ScholarPubMed
Janghorbani, M, Ting, BTG and Young, VR (1980) Accurate analysis of stable isotopes 68Zn, 70Zn and 58Fe in human feces with neutron activation analysis. Clinica Chimica Acta 108, 924.CrossRefGoogle ScholarPubMed
Jasani, BM and Fletcher, J (1972) Determination of iron absorption from measurement of radioactive iron and a non-absorbed radioactive marker in a single faecal sample. Scandinavian Journal of Haematology 9, 547551.Google Scholar
Jasani, BM, Tonge, JL, Fletcher, J and Barnaby, CF (1971) Measurement of the amount of ingested iron temporarily retained in the intestine. British Journal of Haematology 20, 131137.Google Scholar
Johnson, PE, Stuart, MA, Hunt, JR, Mullen, L and Starks, L (1988) 65Copper absorption by women fed intrinsically and extrinsically labelled goose meat, goose liver, peanut butter and sunflower butter. Journal of Nutrition 118, 15221528.CrossRefGoogle ScholarPubMed
Kotb, AR and Luckey, TD (1972) Markers in nutrition. Nutrition Abstracts and Reviews 42, 813844.Google ScholarPubMed
Kramsch, DM, Aspen, AJ and Apstein, CS (1980) Suppression of experimental atherosclerosis by the Ca++-antagonist lanthanum. Journal of Clinical Investigation 65, 967981.CrossRefGoogle ScholarPubMed
Laul, JC, Gosselin, DC, (1989) Radioanalytical methods for REE in geological and biological materials. In Lanthanide Probes in Life, Chemical and Earth Sciences - Theory and Practice, pp. 379388 [Bunzli, CG and Choppin, GR, editors]. Amsterdam: Elsevier.Google Scholar
Laul, JC, Lepel, EA and Smith, MR (1988) Trace rare-earth elemen analysis of briny groundwaters. Journal of Radioanalytical and Nuclear Chemistry 123, 349363.CrossRefGoogle Scholar
Luckey, TD (1979) Whither, intestinal microecology?. American Journal of Clinical Nutrition 32, 109112.Google Scholar
Luckey, TD (1974) Introduction: the villus in chemostat man. American Journal of Clinical Nutrition 27, 12661276.Google Scholar
Luckey, TD, Kotb, A, Vogt, JR and Hutcheson, DP (1975) Feasibility studies in rats fed heavy metals as multiple nutrient markers. Journal of Nutrition 105, 660669.CrossRefGoogle ScholarPubMed
Luckey, TD, Venugopal, B, Gray, D and Hutcheson, D (1977) Lanthanide marker evidence for one and two physiologic compartments in the human alimentary tract. Nutrition Report International 16, 339347.Google Scholar
Magnusson, B, Bjorn-Rasmussen, E, Hallberg, L and Rossander, L (1981) Iron absorption in relation to iron status. Scandinavian Journal of Haematology 27, 201208.CrossRefGoogle ScholarPubMed
Marx, JJM (1979) Mucosal uptake, mucosal transfer, and retention of iron, measured by whole body counting. Scandinavian Journal of Haematology 23, 293302.CrossRefGoogle ScholarPubMed
Mertz, W (1987) Use and misuse of balance studies. Journal of Nutrition 117, 18111813.Google Scholar
Metcalf, AM, Phillips, SF, Zinsmeister, AR, MacCarty, RL, Beart, RW and Wolfe, BG (1987) Simplified assessment of segmental colonic transit. Gastroenterology 92, 4047.CrossRefGoogle ScholarPubMed
Morgan, JB (1986) Use of non-absorbable markers in studies of human nutrient absorption. Human Nutrition: Applied Nutrition 40A, 399411.Google Scholar
O'Dell, BL (1984) Bioavailability of trace elements. Nutrition Reviews 42, 301308.CrossRefGoogle ScholarPubMed
Payton, KB, Flanagan, PR, Stinson, EA, Chodirker, DP, Chamberline, MJ and Valberg, LS (1982) Technique for determination of human zinc absorption from measurement of radioactivity in a fecal sample or the body. Gastroenterology 83, 12641270.CrossRefGoogle ScholarPubMed
Pietra, R, Sabbioni, E, Ubertalli, L, Orvini, E, Vocatura, G, Colombo, F and Zanoni, M (1985) Trace elements in tissues of a worker affected by rare earths pneumoconiosis: a study carried out by neuron activation analysis. Journal of Radioanalytical and Nuclear Chemistry 92, 247259.CrossRefGoogle Scholar
Powell, LW, Campbell, CB and Wilson, E (1970) Intestinal mucosal uptake of iron and iron retention in idiopathic haemochromatosis as evidence for a mucosal abnormality. Gut 11, 727731.CrossRefGoogle ScholarPubMed
Solomons, NW, Janghorbani, M, Ting, BTG, Steinke, FH, Christensen, M, Bijlani, R, Istfan, N and Young, VR (1982) Bioavailability of zinc from a diet based on isolated soy protein: application in young men of the stable isotope tracer 70Zn. Journal of Nutrition 112, 18091821.CrossRefGoogle ScholarPubMed
Talley, NJ, Zinsmeister, AR, Van Dyke, C and Melton, LJ (1991) Epidemiology of colonic symptoms and irritable bowel syndrome. Gastroenterology 101, 927934.CrossRefGoogle ScholarPubMed
Turnlund, JR, Durkin, N, Costa, F and Margen, S (1986) Stable isotope studies of zinc absorption and retention in young and elderly men. Journal of Nutrition 116, 12391247.Google Scholar
Turnlund, JR, Keyes, WR, Hudson, CA, Betschart, AA, Kretsch, MJ and Souberlich, HE (1991) A stable-isotope study of zinc, copper, and iron absorption and retention by young women fed vitamin B-6-deficient diets. American Journal of Clinical Nutrition 54, 10591064.CrossRefGoogle ScholarPubMed
Ulusoy, U (1996) Determination of intestinal uptake of essential trace elements using stable isotopic tracers and rare earth markers PhD Thesis.Google Scholar
Venugopal, B, & Luckey, TD, (1975) Toxicology of non-radioactive heavy metals and their salts. Environmental Quality and Safety, Suppl. 1, PP. 7580 [Coulston, F and Korte, F, editors]. London: Academic Press.Google Scholar