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Prevention of experimental autoimmune encephalomyelitis in Lewis rats by a novel fungal source of γ-linolenic acid

Published online by Cambridge University Press:  09 March 2007

L. S. Harbige ,
N. Yeatman ,
S. Amor  and
M. A. Crawford
L. S. Harbige
Affiliation:
Immunology Department, United Medical and Dental Schools of Guy's and St Thomas' Hospitals, The Rayne Institute, St Thomas' Hospital, London SE1 7EH
N. Yeatman
Affiliation:
Immunology Department, The Royal London Hospital Medical College, London El 2AB
S. Amor
Affiliation:
Immunology Department, United Medical and Dental Schools of Guy's and St Thomas' Hospitals, The Rayne Institute, St Thomas' Hospital, London SE1 7EH
M. A. Crawford
Affiliation:
Institute of Human Nutrition and Brain Chemistry, Queen Elizabeth Hospital for Children, Hayward Building (3rd Floor), Hackney Road, London E2 8PS
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Abstract

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The effects of oral administration of linoleic- and γ-inolenic-acid-rich oils on the clinical and histopathological manifestations of experimental autoimmune encephalomyelitis (EAE) were investigated in Lewis rats 7 d post-inoculation. γ-Linolenic-acid-rich fungal (Mucor javanicus) oil at 500 mg/kg body weight abrogated clinical and histological signs of EAE although at doses of 200 and 1000 mg/kg body weight it was only effective in delaying the onset of clinical disease. Linoleic-acid-rich safflower-seed (Carthamus tinctorius) oil at 500, 750 and 1000 mg/kg body weight decreased the severity of clinical EAE. disease in a dose-dependent manner. The effects in healthy animals of orally administered γ-linolenic-acid-rich fungal oil (500 mg/kg body weight) and linoleic-acid-rich safflower-seed oil (1000 mg/kg body weight) on splenic lymphocyte proliferative responses to the T-cell mitogen concanavalin-A (Con A), membrane fatty acid composition and lymphocyte sub-sets were also studied. Both treatments enhanced the T-cell proliferative response to Con A. There was no significant effect on the proportion of splenic CD8+ or CD4+ lymphocytes. Compositional studies on splenic phosphoglyceride fatty acids of oil-treated animals suggest the above responses were associated with increases in spleen dihomo-γ-linolenic and arachidonic acids.

Keywords

Linoleic acidγ-Linolenic acidExperimental autoimmune encephalomyelitis
Type
γ-Linolenic acid and encephalomyelitis
Information
British Journal of Nutrition , Volume 74 , Issue 5 , November 1995 , pp. 701 - 715
Copyright
Copyright © The Nutrition Society 1995

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