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Prediction of the suitability of soya-bean products for feeding to preruminant calves by an in-vitro immunochemical method

Published online by Cambridge University Press:  24 July 2007

J. W. Sissons ,
R. H. Smith ,
D. Hewitt  and
A. Nyrup
J. W. Sissons
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
R. H. Smith
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
D. Hewitt
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
A. Nyrup
Affiliation:
Aarhus Oliefabrik A/S, PO Box 50, DK-8100 Aarhus C., Denmark
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Abstract

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1. An immunochemical test for predicting the suitability of different soya-bean products for feeding to preruminant calves was studied. Calves fitted with abomasal and re-entrant ileal cannulas, were first given a series of feeds containing heated soya-bean flour and two with casein as the protein source, and then given further test feeds in which different experimental soya-bean products, prepared under conditions which might be used commercially, provided the nitrogren source. These products (Q, R, S and T) were prepared respectively by extracting fat-free soya-bean flour with aqueous ethanol at 960, 650, 650 and 550 ml/l and at 60, 60, 78 and 78° and then treating the residue with steam for 30 min.

2. After infusion of a test feed into the abmasum measurements were made of transit time through the small intestine, flow-rate of ileal digesta, recovery of polyethylene glycol (a water-soluble marker added to the feed) and net N absorption up to the distal ileum. Glycinin and β-conglycinin, antigenic constituents of soya-bean protein, were determined in saline (9 g sodium chloride/l) extracts of soya-bean products by haemagglutination inhibition assay.

3. After calves had been given a series of four feeds containing heated soya-bean flour, they showed hypersensitivity to the flour by developing disturbances in digesta movement and N uptake. When experiments were made to compare the effects of giving feeds based on casein with those containing products R, S and T it was shown that some differences in transit times and flow-rates of digesta through, and N absorption from, the small intestine occurred. Disturbances were, however, much less than those observed with product Q feeds.

4. Haemaggultination inhibition assay of products Q, R, S and T gave titres (log2 reciprocal of highest dilution of saline extract inhibiting agglutination) of 12, 5, 3 and 2 for glycinin and 13, 3, 3 and 2 for β-conglycinin respectively. After steam treatment only product Q gave measurable titres; 10 and 11 for glycinin and β-conglycinin respectively.

5. It is concluded that the haemagglutination inhibition assay is a suitable in vitro method of indicating whether soya-bean products given in liquid feeds to preruminant calves will cause gastrointestinal allergic reactions. Furthermore, results show that the removal of deleterious factors from soya-bean meal by treating with ethanol, is critically dependent on the proportion of water present in the ethanol and the temperature of the treatment.

Type
Research Article
Information
British Journal of Nutrition , Volume 47 , Issue 2 , March 1982 , pp. 311 - 318
Copyright
Copyright © The Nutrition Society 1982

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