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Postprandial lipaemia is associated with increased levels of apolipoprotein A-IV in the triacylglycerol-rich fraction and decreased levels in the denser plasma fractions

Published online by Cambridge University Press:  09 March 2007

Jean Dallongeville ,
Pascal Lebel ,
Henri-Joseph Parra ,
Gérald Luc  and
Jean-Charles Fruchart
Jean Dallongeville
Affiliation:
SERLIA et Unité LVSERM 325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cédex, France
Pascal Lebel
Affiliation:
SERLIA et Unité LVSERM 325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cédex, France
Henri-Joseph Parra
Affiliation:
SERLIA et Unité LVSERM 325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cédex, France
Gérald Luc
Affiliation:
SERLIA et Unité LVSERM 325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cédex, France
Jean-Charles Fruchart
Affiliation:
SERLIA et Unité LVSERM 325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cédex, France
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Abstract

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Apolipoprotein (apo) A-IV is primarily associated with HDL or with the lipoprotein-free fraction of plasma, and in small amounts with chylomicrons and VLDL. The aim of the present study was to assess the effect of a fatty meal on the postprandial variation in plasma apo A-IV and on its distribution among lipoprotein fractions following absorption of fat. Twenty healthy male subjects participated in the study. After an overnight fast, subjects were given a fatty breakfast containing 1 g fat/kg body weight (% energy: fat 65, carbohydrate 20, protein 15). Blood samples were taken every hour during the next 10 h. Apo A-IV was measured by ELISA. Postprandial lipaemia was associated with a moderate, although significant, increase in the plasma levels of apo A-IV. Apo A- IV increased from the median baseline value of 0·15 g/1 to 0·165 g/l (median + 17 %; P < 0·01) 5 h after fat ingestion. The postprandial peak of apo A-IV occurred 1 h after the triacylglycerol peak. There were no statistically significant correlations between baseline lipids, baseline apo A-IV and postprandial changes in apo A-IV levels, or between postprandial changes in lipids and apo A-IV at any time. To assess apo A-IV distribution among lipoproteins, plasma was fractionated by fast performance liquid chromatography at baseline and 3, 6 and 10 h postprandially. There was a substantial heterogeneity in the apo A-IV distribution among lipoproteins following the fatty meal. At 3 h after fat ingestion, apo A-IV levels increased in the triacylglycerol-rich lipoprotein (TRL) fraction and decreased in the denser plasma fraction. At 6 h after the fatty meal, apo A-IV was still present in the TRL but was decreased in the HDL fractions. The findings of the present study support the concept that apo A-IV particles transfer from the denser plasma fraction to TRL during postprandial lipaemia

Keywords

Postprandial metabolismApolipoprotein A-IVLipids
Type
Human and Clinical Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 2 , February 1997 , pp. 213 - 223
Copyright
Copyright © The Nutrition Society 1997

References

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