Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-22T21:02:23.849Z Has data issue: false hasContentIssue false
  • English
  • Français

Portal net appearance of amino acids in growing pigs fed a barley-based diet with inclusion of three different forage meals

Published online by Cambridge University Press:  09 March 2007

M. Reverter ,
T. Lundh ,
H. L. Gonda  and
J. E. Lindberg
M. Reverter
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, PO Box 7024x, S-750 07 Uppsala, Sweden
T. Lundh
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, PO Box 7024x, S-750 07 Uppsala, Sweden
H. L. Gonda
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, PO Box 7024x, S-750 07 Uppsala, Sweden
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The net absorption of amino acids (AA) in young pigs fed a barley-based control diet (C) and diets where barley was replaced by 200 g/kg fresh weight of dried lucerne (Medicago sativa; L20), white clover (Trifolium repens; W20) or perennial ryegrass (Lolium perenne; PR20) meal was studied. Castrated male pigs were fitted with permanent catheters in the hepatic portal vein and mesenteric artery, and the hepatic portal net absorption of AA was estimated from the porto–arterial plasma concentration differences and the hepatic portal-vein blood flow. In general, the essential AA (EAA) concentrations in the hepatic portal vein reached peak levels 90 min after feeding and thereafter exhibited a transient decline. Maximum porto–arterial differences were reached between 1 and 3 h postprandially for most of the AA. The cumulative net absorption of non-essential AA (NEAA) and EAA did not differ significantly between the barley-based diet and diets W20 and PR20. Due to a lower intake of AA on diet L20, the cumulative net absorption of NEAA and EAA was significantly (P<0·05) lower than diet C. With the exceptions of the EAA arginine, cystine and valine, and the NEAA glutamic acid + glutamine and glycine, there were no significant differences in the absorption coefficients for the EAA and NEAA between the diets. In addition, the pattern of the total EAA in the mixture absorbed postprandially did not differ significantly between the diets. The present study gives support to the contention that the replacement of barley AA with forage meal AA in a barley-based diet for growing pigs should be expected to result in minor differences in the net portal flux of AA.

Keywords

Portal veinAmino acid absorptionFibreForage
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 4 , October 2000 , pp. 483 - 494
Copyright
Copyright © The Nutrition Society 2000

References

Aliev, AA, Ataev, UI and Blinov, VI (1978) Synthesis of plasma proteins in the gastrointestinal tract of animals. Vestnik Sel'skohoziaistvennoi Nauki/Vashni, USSR 1, 54–62.Google Scholar
Andersson, C, (1997) Forages for growing pigs – partition of digestion and nutritive value. PhD Thesis, Swedish University of Agricultural Sciences. Acta Universitatis Agriculturae Sueciae, Agraria 31.Google Scholar
Andersson, C and Lindberg, JE (1997) Forages in diets for growing pigs. 1. Nutrient apparent digestibilities and partition of nutrient digestion in barley-based diets with inclusion of lucerne and white clover meal. Animal Science 65, 483–491.CrossRefGoogle Scholar
Andersson, C and Lindberg, JE (1997) Forages in diets for growing pigs. 2. Nutrient apparent digestibilities and partition of nutrient digestion in barley-based diets including red-clover and perennial ryegrass meal. Animal Science 65, 493–500.CrossRefGoogle Scholar
Bergner, H, Simon, O and Zimmer, M (1975) Einfluß des Gehaltes nativer Rohfaser in Diaeten von Ratten auf die Aminosäurenresoption (Influence of crude fibers in the diet of rats on the absorption of amino acids). Archives für Tierernährung 25, 95–104.CrossRefGoogle Scholar
Biolo, G, Tessari, P, Inchiostro, S, Bruttomesso, D, Frongher, C, Sabadin, L, Fratton, MG, Valerio, A and Tiengo, A (1992) Leucine and phenylalanine kinetics during mixed meal ingestion: a multiple tracer approach. American Journal of Physiology 262, 455–463.Google ScholarPubMed
Backwell, FRC, Bequette, BJ, Wilson, D, Metcalf, JA, Franklin, MF, Beever, DE, Lobley, GE and MacRae, JC (1996) Evidence for the utilization of peptides for milk protein synthesis in the lactating dairy goat. in vivo. American Journal of Physiology 271, R955–R960.Google Scholar
Cohen, SA and De Antonis, KM (1994) Applications of amino acid derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. Analysis of feed grains, intravenous solutions and glycoproteins. Journal of Chromatography 661A, 25–34.Google Scholar
Galibois, I, Simoes, Nunes C, Rérat, A and Savoie, L (1989) Net appearance of amino acids in portal blood during the digestion of casein or rapeseed meal proteins in the pig. Canadian Journal of Physiology and Pharmacology 67, 1409–1417.Google Scholar
Hayes, KC (1985) Taurine requirement in primates. Nutrition Reviews 43, 65–70.Google Scholar
Koeln, LL, Schlagheck, TG and Webb, KE (1993) Amino acid flux across the gastrointestinal tract and liver of calves. Journal of Dairy Science 76, 2275–2285.Google Scholar
Lenis, NP, Bikker, P, van der Meulen, J, Van Diepen, JThM, Bakker, JGM and Jongbloed, AW (1996) Effect of dietary neutral detergent fiber on ileal digestibility and portal flux of nitrogen and amino acids and on nitrogen utilization in growing pigs. Journal of Animal Science 74, 2687–2699.CrossRefGoogle ScholarPubMed
Lindberg, JE and Andersson, C (1998) The nutritive value of barley-based diets with forage meal inclusion for growing pigs based on total tract digestibility and nitrogen utilization. Livestock Production Science 56, 43–52.CrossRefGoogle Scholar
Lindberg, JE and Cortova, Z (1995) The effect of increasing inclusion of lucerne leaf meal in a barley-based diet on the partition of digestion and on nutrient utilization in pigs. Animal Feed Science and Technology 56, 11–20.CrossRefGoogle Scholar
Lindberg, JE, Cortova, Z and Thomke, S (1995) The nutritive value of lucerne leaf meal for pigs based on digestibility and nitrogen utilization. Acta Agriculturae Scandinavica 45A, 245–251.CrossRefGoogle Scholar
Malmöf, K, Simoes, Nunes C and & Ouml;rberg, J (1988) Effects of high dietary fibre level on postprandial porto-arterial differences in the plasma concentrations of immnoreactive insulin, glucose and free amino acids in the growing pig. Swedish Journal of Agricultural Research 18, 67–75.Google Scholar
Matthews, DE, Marano, MA and Campbell, RG (1993) Splanchnic bed utilization of leucine and phenylalanine in humans. American Journal of Physiology 264, 109–118.Google Scholar
Moore, S (1963) On the determination of cystine as cysteic acid. Journal of Biological Chemistry 238, 235–237.Google Scholar
Patterson, DH & Lucas, HL (1962) Change-over designs. Technical Bulletin no. 147. Raleigh, NC: North Carolina State College Agricultural Experimental Station.Google Scholar
Pion, R, Fauconneau, C and Rérat, A (1964) Variation de la composition en acides aminés du sang porte au cours de la digestion chex le porc (Variations in the amino acid composition of the portal blood during digestion in the pig). Annales de Biologie Animale Biochimie Biophysique 4, 383–402.CrossRefGoogle Scholar
Rérat, A (1980) Some quantitative aspects of protein and carbohydrate absorption in the pig. Proceedings of the Nutrition Society 39, 177–184.CrossRefGoogle ScholarPubMed
Rérat, A, (1982) Absorption des sucres et des acides aminés chez le porc (Sugar and amino acid absorption in the pig). In Physiologie Digestive chez le Porc, Les Colloques de l'INRA no 12, pp. 63–85.[Laplace, JP, Corring, T and Rérat, A editors]. Paris: Instiut National de la Recherche Agronomique.Google Scholar
Rérat, A (1990) Absorption of nitrogen and amino acids from exogenous (fish meal proteins) or endogenous sources in the pig. Pig News and Information 11, 173–180.Google Scholar
Rérat, A, Corring, T, (1991) Animal factors affecting protein digestion and absorption. In Proceedings of the Vth International Symposium on Digestive Physiology in Pigs. European Association for Animal Production publication. 5–34.[Verstegen, MWA, Huisman, J and Aden, Hartog L editors].Wageningen: Pudoc.Google Scholar
Rérat, A, Corring, T, Laplace, JP, (1976) Protein digestion and absorption Protein Metabolism and Nutrition. European Association for Animal Production Publication 16 97–138. [Cole, DJA, Boorman, KN, Buttery, PJ, Lewis, D, Neale, RJ and Swan, H editors]. London: Butterworths.Google Scholar
Rérat, A, Jung, J and Kandé, J (1988) Absorption kinetics of dietary hydrolysis products in conscious pigs given diets with different amounts of fish protein. 2. Individual amino acids. British Journal of Nutrition 60, 105–120.CrossRefGoogle ScholarPubMed
Rérat, A, Vaissade, P and Vaugelade, P (1979) Absorption kinetics of amino acids and reducing sugars during digestion of barley or wheat meals in the pig: Preliminary data. Annales de Biologie Animale Biochemie Biophysique 19, 739–747.CrossRefGoogle Scholar
Rérat, A, Vaissade, P and Vaugelade, P (1988) Absorption kinetics of dietary hydrolysis products in conscious pigs given diets with different amounts of fish protein. 1. Amino-nitrogen and glucose. British Journal of Nutrition 60, 91–104.CrossRefGoogle ScholarPubMed
Rérat, A, Vaugelade, P, Villiers, P (1980) A new method for measuring the absorption of nutrients in the pig: critical examination. In Current Concepts of Digestion and Absorption in Pigs, 177–217. [Low, AG and Partridge, IG editors]. Reading, Berks: NIRD.Google Scholar
Reverter, M and Lindberg, JE (1998) Ileal digestibility of amino acids in pigs given a barley-based diet with increasing inclusion of lucerne leaf meal. Animal Science 67, 131–138.Google Scholar
Reverter, M, Lundh, T and Lindberg, JE (1997) Determination of free amino acids in pig plasma by precolumn derivatization with 6-N-aminoquinolyl-N-succinimidyl carbamate and high-performance liquid chromatography. Journal of Chromatography 696B, 1–8.Google Scholar
Reverter, M, Lundh, T and Lindberg, JE (1999) Ileal amino acid digestibilities in pigs of barley-based diets with inclusion of lucerne (Medicago sativa), white clover (Trifolium repens), red clover (Trifolium pratense) and perennial ryegrass (Lolium perenne). British Journal of Nutrition 82, 139–147.Google Scholar
Simoes, Nunes C, Galibois, I, Rérat A, Savoie, L and Vaugelade, P (1991) Hepatic and portal-drained viscera balances of amino acids, insulin, glucagon and gastrin in the pig after the ingestion of casein or rapeseed proteins. Reproduction, Nutrition, Development 31, 217–231.CrossRefGoogle Scholar
Vestergaard, EM, Danielsen, V, Eklundh, Larsen A & Bejerholm, C (1996) Grønmel til slagtesvin og draegtige søer (Dried grass meal for finishing pigs and pregnant sows). Statens Husdyrbrugsforsøg. Landbrugs- og Fiskeriministeriet. Forskingsrapport no. 50. Tjele, Denmark: Danish Institute of Animal Science.Google Scholar
Wiesemüller, W and Poppe, S (1990) Lucerne silage as a feedstuff for sows. World Review of Animal Production 25, 47–53.Google Scholar
Windmueller, HG and Spaeth, AE (1974) Uptake and metabolism of plasma glutamine by the small intestine. Journal of Biological Chemistry 249, 5070–5079.CrossRefGoogle ScholarPubMed
Windmueller, HG and Spaeth, AE (1976) Metabolism of absorbed aspartate, asparagine and arginine by rat small intestine in vivo. Archives of Biochemistry and Biophysics 175, 670–676.Google Scholar
Wu, G (1997) Synthesis of citrulline and arginine from proline in enterocytes of postnatal pigs. American Journal of Physiology 277, 1382–1390.Google Scholar
Wu, G (1998) Intestinal mucosal amino acid catabolism. Journal of Nutrition 128, 1249–1252.CrossRefGoogle ScholarPubMed
Yu, YM, Wagner, DA, Tredget, EE, Walaszewski, JA, Burke, JF and Young, VR (1990) Quantitative role of splanchnic region in leucine metabolism: L-[1-13C, 15N]leucine and substrate balance studies. American Journal of Physiology 259, E36–E51.Google ScholarPubMed