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Polyunsaturated fatty acids and conjugated linoleic acid isomers in breast milk are associated with plasma non-esterified and erythrocyte membrane fatty acid composition in lactating women

Published online by Cambridge University Press:  08 March 2007

Alexandre G. Torres ,
Jacqueline G. Ney ,
Flávia Meneses  and
Nádia M. F. Trugo
Alexandre G. Torres*
Affiliation:
Universidade Federal do Rio de Janeiro, Cidade Universitária, Laboratório de Bioquímica Nutricional e de Alimentos, Instituto de Química, CT/ Bl. A, 21949–900, Rio de JaneiroBrazil
Jacqueline G. Ney
Affiliation:
Universidade Federal do Rio de Janeiro, Cidade Universitária, Laboratório de Bioquímica Nutricional e de Alimentos, Instituto de Química, CT/ Bl. A, 21949–900, Rio de JaneiroBrazil
Flávia Meneses
Affiliation:
Universidade Federal do Rio de Janeiro, Cidade Universitária, Laboratório de Bioquímica Nutricional e de Alimentos, Instituto de Química, CT/ Bl. A, 21949–900, Rio de JaneiroBrazil
Nádia M. F. Trugo
Affiliation:
Universidade Federal do Rio de Janeiro, Cidade Universitária, Laboratório de Bioquímica Nutricional e de Alimentos, Instituto de Química, CT/ Bl. A, 21949–900, Rio de JaneiroBrazil
*
*Corresponding authorDr Alexandre G. Torres, fax +55 21 2562 7266, email [email protected]
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Abstract

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Maternal adipose tissue is a major contributor to breast milk long-chain fatty acids, probably through the pool of plasma NEFA. The fatty acid composition of the erythrocyte membrane (EM) is a biochemical index of the intake of fatty acids not synthesized endogenously and of PUFA and long-chain PUFA fatty acid status. The present study investigated the associations between breast milk fatty acid composition and the composition of plasma NEFA and of EM fatty acids with special reference to PUFA, long-chain PUFA and conjugated linoleic acid (CLA). The detailed fatty acid composition of mature breast milk was also reported. Thirty-three healthy, lactating Brazilian women donated milk samples; of these, twenty-four also donated blood samples in an observational cross-sectional study. Breast milk fatty acid composition presented several associations with NEFA and EM composition, which explained most (≥50%) of the variability of selected milk PUFA, long-chain PUFA and CLA. Milk CLA was associated with fatty acids that are markers of dairy fat intake in the diet, NEFA and EM. In general, breast milk n-3 fatty acids and CLA, but not n-6 fatty acids, were associated with EM composition, whereas both the n-6 and n-3 fatty acids and CLA in milk were associated with NEFA composition, possibly owing to its role as a direct source of fatty acids for breast milk. These findings emphasize the contribution of the NEFA pool derived from the adipose tissue to the long-chain fatty acid composition of breast milk.

Keywords

Fatty acid metabolismNon-esterified fatty acidsErythrocyte fatty acidsLactation
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 3 , March 2006 , pp. 517 - 524
Copyright
Copyright © The Nutrition Society 2006

References

Bligh, EG&Dyer, WJA 1959 A rapid method of total lipid extraction and purification.. Can J Biochem Physiol 37 911–917.CrossRefGoogle ScholarPubMed
Broekhuyse, RM 1974 Long-term storage of erythrocytes for quantitative analyses of lipids. Clin Chim Acta 52 53–58.CrossRefGoogle ScholarPubMed
Brossard, NCroset, MNormand, SPousin, JLecerf, JLaville, MTayot, JL &Lagarde, M 1997 Human plasma albumin transports [13C]docosahexaenoic acid in two lipid forms to blood cells.. J Lipid Res 38 1571–1582.CrossRefGoogle ScholarPubMed
Butte, NF&Hopkinson, JM 1998 Body composition changes during lactation are highly variable among women. J Nutr 128 381S–385S.CrossRefGoogle ScholarPubMed
Butte, NFHopkinson, JMMehta, NMoon, JK & Smith, EO'B 1999 Adjustments in energy expenditure and substrate utilization during late pregnancy and lactation.. Am J Clin Nutr 69 299–307.CrossRefGoogle ScholarPubMed
Calabro, MAPrasad, MRWakil, SJ&Joshi, VC 1982 Stearoyl-coenzyme A desaturase activity in the mammary gland and liver of lactating rats.. Lipids 17 397–402.CrossRefGoogle ScholarPubMed
Cunha, Jda Costa, THM&Ito, MKInfluences of maternal dietary intake and suckling on breast milk lipid and fatty acid composition in low-income women from Brasýlia, Brazil.. Early Human Dev 2005 81 303–311.CrossRefGoogle Scholar
Demmelmair, HBaumheuer, MKoletzko, BDokoupil, K &Kratl, G 1998 Metabolism of U13C-labeled linoleic acid in lactating women.. J Lipid Res 39 1389–1396.CrossRefGoogle ScholarPubMed
Hachey, DLThomas, MREmken, EAGarza, CBrown-Booth, LAdlof, RO & Klein, PD 1987 Human lactation: maternal transfer of dietary triglycerides labeled with stable isotopes.. J Lipid Res 28 1185–1192.CrossRefGoogle ScholarPubMed
Hellerstein, MKChristiansen, MKaempfer, SKletke, CWu, K&Reid, JS 1991 Measurement of de novo hepatic lipogenesis in humans using stable isotopes.. J Clin Invest 87 1841–1852.CrossRefGoogle ScholarPubMed
Hunter, D Biochemical indicators of dietary intake. In Nutritional Epidemiology, 2nd ed. New York:[W Willett, editor]. Oxford University Press. 1998 174–243.Google Scholar
Innis, SM 1992 Plasma and red blood cell fatty acid values as indexes of essential fatty acids in the developing organs of infants fed with milk or formulas.. J Pediatr 120 78S–86S.CrossRefGoogle ScholarPubMed
Jensen, RG 1999 Lipids in human milk.. Lipids 34 1243–1271.CrossRefGoogle ScholarPubMed
Jensen, RGBitman, JCarlson, SECouch, SCHamosh, M&Newburg, DSMilk lipids: A. Human milk lipids.In Handbook of Milk Composition San Diego, CA:[RG Jensen, editor]. Academic Press. 1995 495–575.Google Scholar
Jensen, RG, Lammi-Keefe, C & Koletzko, B 1997 Representative sampling of human milk and the extraction of fat for analysis of environmental lipophilic contaminants. Toxicol Environ Chem 62 229–247.CrossRefGoogle Scholar
Jones, PJH & Kubow, SLipids, sterols, and their metabolites. In Modern Nutrition in Health and Disease 9th ed. Shils, ME, Olson, JA, Shike, M & Ross, ACPennsylvania: Williams & Wilkins 1999Google Scholar
Katan, MB, Deslypere, JP, van Birgelen, PJM, Penders, M & Zegwaard, M 1997 Kinetics of the incorporation of dietary fatty acids into serum cholesteryl esters, erythrocyte membranes, and adipose tissue: an 18-month controlled study. J Lipid Res 38 2012–2022.CrossRefGoogle ScholarPubMed
Koletzko, B & Rodriguez-Palmero, M 1999 Polyunsaturated fatty acids in human milk and their role in early infant development. J Mammary Gland Biol Neoplasia 4 269–284.CrossRefGoogle ScholarPubMed
Kramer, JKG, Fellner, V, Dugan, MER, Sauer, FD, Mossoba, MM & Yurawecz, MP 1997 Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids. Lipids 32 1219–1228.CrossRefGoogle ScholarPubMed
Lepage, G & Roy, CC 1986 Direct transesterification of all classes of lipids in a one-step reaction. J Lipid Res 27 114–120.CrossRefGoogle Scholar
Lin, H, Boylston, TD, Chang, MJ, Luedecke, LO & Shultz, TD 1995 Survey of the conjugated linoleic acid content of dairy products. J Dairy Sci 78 2358–2365.CrossRefGoogle ScholarPubMed
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RJ 1951 Protein measurement with the Folin phenol reagent. J Biol Chem 193 265–275.CrossRefGoogle ScholarPubMed
Macdiarmid, J & Blundell, J 1998 Assessing dietary intake: who, what and why of under-reporting. Nutr Res Rev 11 231–253.CrossRefGoogle ScholarPubMed
Marangoni, F, Agostoni, C, Lammardo, AM, Bonvissuto, M, Giovannini, M, Galli, C & Riva, E 2002 Polyunsaturated fatty acids in maternal plasma and in breast milk. Prostaglandins Leukot Essent Fatty Acids 66 535–540.CrossRefGoogle ScholarPubMed
Martin, J-C, Bougnoux, P, Fignon, A, Theret, V, Antoine, JM, Lamisse, F & Couet, C 1993 Dependence of human milk essential fatty acids on adipose stores during lactation. Am J Clin Nutr 58 653–659.CrossRefGoogle ScholarPubMed
Monteiro, CA, Mondini, L & Costa, RBL 2000 Mudanças na composição e adequação da dieta familiar nas áreas metropolitanas do Brasil (1988–1996) [Changes in composition and appropriate nutrition of family diet in the metropolitan areas of Brazil (1988–1996)]. Rev Saúde Pućblica 34 251–258.CrossRefGoogle Scholar
Monteiro, CA, Mondini, L, de Souza, AL & Popkin, BM 1995 The nutrition transition in Brazil. Eur J Clin Nutr 49 105–113.Google ScholarPubMed
NEPA/UNICAMP (Núcleo de Estudos e Pesquisas em Alimentos/Universidade Estadual de Campinas) Tabela Brasileira de Composição de Alimentos – TACO [Brazilian Food Composition Table – TACO]. Campinas, Brazil: UNICAMP/ Ministério da Saúde 2004Google Scholar
Neville, MC & Picciano, MF 1997 Regulation of milk lipid secretion and composition. Annu Rev Nutr 17 159–184.CrossRefGoogle Scholar
Ney, JG, Torres, AG & Trugo, NMF 2004 An´lise de ácidos graxos nãoesterificados de plasma humano por cromatografia gasosa capilar cominjeçáo sem divisão de fluxo [Analysis of nonesterified fatty acids in human plasma by capillary gas-chromatography with splitless injection]. Quím Nova 27 561–566.CrossRefGoogle Scholar
Pariza, MW, Park, Y & Cook, ME 2000 Mechanisms of action of conjugated linoleic acid: evidence and speculation. Proc Soc Exp Biol Med 223 8–13.Google ScholarPubMed
Park, Y, McGuire, MK, Behr, R, McGuire, MA, Evans, MA & Shultz, TD 1999 High-fat dairy product consumption increases Δ9c, 11t-18:2 (rumenic acid) and total lipid concentrations of human milk.. Lipids 34 543–549.CrossRefGoogle Scholar
Perry, NA & Doan, FJA 1950 A picric acid method for the simultaneous determination of lactose and sucrose in dairy products. J Dairy Sci 33 176–185.CrossRefGoogle Scholar
Polette, A, Durand, P, Floccard, B & Blache, D 1992 A method for specific analysis of free fatty acids in biological samples by capillary gas chromatography. Anal Biochem 206 241–245.CrossRefGoogle ScholarPubMed
Precht, D & Molkentin, J 1997 Effect of feeding on conjugated cis delta 9, trans delta 11-octadecadienoic acid and other isomers of linoleic acid in bovine milk fats. Nahrung 41 330–335.CrossRefGoogle ScholarPubMed
Rocquelin, G, Tapsoba, S, Dop, MC, Mbemba, F, Traissac, P & Martin-Prével, Y 1998 Lipid content and essential fatty acid (EFA) composition of mature congolese breast milk are influenced by mothers’ nutritional status: impact on infants’ EFA supply.. Eur J Clin Nutr 52 164–171.CrossRefGoogle ScholarPubMed
Silva, MHL, Silva, MTC, Brandão, SCC, Gomes, JC, Peternelli, LA & Franceschini, SCC 2005 Fatty acid composition of mature breast milk in Brazilian women. Food Chem 93 297–303.CrossRefGoogle Scholar
Torres, AG, Meneses, F & Trugo, NMF 2000 Fatty acid content of erythrocyte membrane in Brazilian lactating women and its relation with dietary intake. FASEB J 14 A209Google Scholar
Torres, AG, Meneses, F & Trugo, NMF 2002a Content of conjugated linoleic acids, cis-9, trans-11, 18:2 and trans-10, cis-12, 18:2 in breast milk from Brazilian women: association with milk composition and diet. Adv Exp Med Biol 503 273–274.Google Scholar
Torres, AG, Ney, JG, Ribeiro, M & Trugo, NMF 2004 Plasma nonesteri-fied fatty acid composition is different in lactating and in non-pregnant non-lactating women. Adv Exp Med Biol 554 511–514.CrossRefGoogle Scholar
Torres, AG, Trugo, NMF & Trugo, LC 2002b Mathematical method for the prediction of retention times of fatty acid methyl esters in temperature-programmed capillary gas-chromatography. J Agric Food Chem 50 4156–4163.CrossRefGoogle ScholarPubMed
Trugo, NMF, Donangelo, CMKoury, JC, Silva, MI & Freitas, LA 1988 Concentration and distribution pattern of selected micronutrients in preterm and term milk from urban Brazilian mothers during early lactation. Eur J Clin Nutr 42 497–507.Google ScholarPubMed
United States Department of Agriculture – Agricultural Research Service (2000) USDA Nutrient Database for Standard Reference, Release 13.http://www.nal.usda.gov/fnic/foodcomp (accessed March 2001)Google Scholar
Vlaadingerbroek, H & Hornstra, G 2004 Essential fatty acids in erythrocyte phospholipids during pregnancy and at delivery in mothers and their neonates: comparison with plasma phospholipids. Prostaglandins Leukot Essent Fatty Acids 71 363–374.CrossRefGoogle Scholar
Wolff, RL, Bayard, CC & Fabien, RJ 1995 Evaluation of sequential methods for the determination of butterfat fatty acid composition with emphasis on trans-18:1 acids. Application to the study of seasonal variations in French butters. J Am Oil Chem Soc 72 1471–1483.CrossRefGoogle Scholar
World Health Organization The Use and Interpretation of Anthropometry, Technical Report Series No. 854. Geneva World Health Organization 1995Google Scholar