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Plasma responses in human subjects after ingestions of multiple doses of natural α-cryptoxanthin: a pilot study

Published online by Cambridge University Press:  08 March 2007

Jörg Schlatterer ,
Dietmar E. Breithaupt ,
Maike Wolters  and
Andreas Hahn
Jörg Schlatterer
Affiliation:
Institute of Food Chemistry, University of Hohenheim, Garbenstrasse 28, 70593 Stuttgart, Germany
Dietmar E. Breithaupt*
Affiliation:
Institute of Food Chemistry, University of Hohenheim, Garbenstrasse 28, 70593 Stuttgart, Germany
Maike Wolters
Affiliation:
Institute of Food Science, University of Hannover, Wunstorfer Strasse 14, 30453 Hannover, Germany
Andreas Hahn
Affiliation:
Institute of Food Science, University of Hannover, Wunstorfer Strasse 14, 30453 Hannover, Germany
*
*Corresponding author: Dr Dietmar E. Breithaupt, fax +49 711 4594096, email [email protected]
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Abstract

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Xanthophylls have attracted a lot of interest since their health benefits were documented. Unfortunately, studying their intestinal absorption is often affected by high baseline levels present in the fasting plasma. As α-cryptoxanthin is rarely found in the traditional European diet, its concentration in human plasma is extremely low. A pilot human intervention study was designed using α-cryptoxanthin for the first time as a marker xanthophyll in a minimally formulated cellulose-based supplement. α-Cryptoxanthin was administered in gelatin soft-gel capsules in multiple doses of 156μg/d to three male volunteers (age 27·3 (sd 4·7) years; BMI 21·6 (sd 0·3) kg/m2) for 16d after a 2-week carotenoid depletion period. Fasting blood samples were taken before the intervention and after 3, 6, 9, 13 and 16d. Plasma HPLC analyses allowed for determination of the concentration; liquid chromatography–MS in the single ion monitoring mode was used to confirm peak assignment. The concentrations of α-cryptoxanthin increased significantly after only 3d of supplementation. The concentration-time plots showed a characteristic shape with a first maximum after day 6, a decline until day 9 and a gradual second rise until the end of the study. Standardisation of plasma α-cryptoxanthin concentrations to triacylglycerol or total cholesterol did not influence the characteristics. The maximum concentrations reached at the end of the intervention period ranged from 0·077 to 0·160μmol/l. These results suggest a high intestinal absorption and an enrichment of α-cryptoxanthin in the plasma even from a minimally formulated cellulose-based supplement.

Keywords

α-CryptoxanthinPlasma xanthophyll responseLiquid chromatography–mass spectroscopy in the single ion monitoring modePilot studies
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 2 , August 2006 , pp. 371 - 376
Copyright
Copyright © The Nutrition Society 2006

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