Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-23T03:23:06.694Z Has data issue: false hasContentIssue false
  • English
  • Français

Phytate levels in diverse rat tissues: influence of dietary phytate

Published online by Cambridge University Press:  09 March 2007

F. Grases ,
B. M. Simonet ,
R. M. Prieto  and
J.G March
F. Grases*
Affiliation:
Laboratory of Renal Lithiasis Research, University of Balearic Islands, Palma de Mallorca, Spain
B. M. Simonet
Affiliation:
Laboratory of Renal Lithiasis Research, University of Balearic Islands, Palma de Mallorca, Spain
R. M. Prieto
Affiliation:
Laboratory of Renal Lithiasis Research, University of Balearic Islands, Palma de Mallorca, Spain
J.G March
Affiliation:
Laboratory of Renal Lithiasis Research, University of Balearic Islands, Palma de Mallorca, Spain
*
*Corresponding author: Dr Felix Grases, fax +34 971 17 34 26, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Phytate (inositol hexaphosphate; InsP6) was determined in rat tissues fed on diets with different phytate contents, using a GC–mass detection methodology that permitted the evaluation of the total amount of this substance present in such tissues. The highest InsP6 concentrations were found in brain (5·89×10-2 (SE 5·7×10-3) MG/G DM), WHEREAS THE CONCENTRATIONS DETECTED IN KIDNEYS, LIVER AND BONE WERE SIMILAR TO EACH OTHER (1·96×10-3 (se 0·20×10-3), 3·11×10-3 (se 0·24×10-3), 1·77×10-3 (se 0·17×10-3) mg/g DM respectively) and 10-fold less than those detected in brain. When rats were fed on a purified diet in which InsP6 was undetectable, the InsP6 levels of the organs mentioned earlier decreased dramatically (9·0×10-4, 3·8×10-5, 1·4×10-5 mg/g DM in brain, kidneys and liver respectively) and in some cases became undetectable (bone). The addition of InsP6 to this purified diet led to the increase of InsP6 levels in these tissues. This clearly demonstrated that the majority of the InsP6 found in organs and tissues has a dietary origin and is not a consequence of endogenous synthesis. Consequently, considering that InsP6 could be involved in some important biological roles, the value of any diet on supplying this substance is noteworthy.

Keywords

PhytateBoneCalciumZinc
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 2 , August 2001 , pp. 225 - 231
Copyright
Copyright © The Nutrition Society 2001

References

Anonymous (1987) Phytic acid: new doors open for a chelator. Lancet 19, 664–666.Google Scholar
Brearley, CA & Hanke, DE (1996) Metabolic evidence for the order of addition of individual phosphates esters in the myo-inositol moiety of inositol hexakisphosphate in the duckweed Spirodela polyrhiza L. Biochemical Journal 314, 227–233.CrossRefGoogle ScholarPubMed
Bunce, CM, French, PJ, Allen, P, Mountford, JC, Moor, B, Greaves, MF, Michell, RH & Brown, G (1993) Comparison of the levels of inositol metabolites in transformed haemopoietic cells and their normal counterparts. Biochemical Journal 289, 667–673.Google Scholar
Conte, A, Pizá, P, García-Raja, A, Grases, F, Costa-Bauzá, A & Prieto, RM (1999) Test of urinary lithogen risk: usefulness in the evaluation of renal lithiasis treatment using crystallisation inhibitors (citrate and phytate). Archivos Españoles de Urologia 52, 94–99.Google ScholarPubMed
Davidsson, L, Galan, P, Cherouvrier, F, Kastenmayer, P, Juillerat, MA, Hercberg, S & Hurrell, RF (1997) Bioavailability in infants of iron from infant cereals: effect of dephytinization. American Journal of Clinical Nutrition 65, 916–920.CrossRefGoogle ScholarPubMed
Efanov, AM, Zaitsev, SV & Berggren, P (1997) Inositol hexakisphosphate stimulates non-Ca2+-mediated exocytosis of insulin by activation of protein kinase C. Proceedings of the National Academy of Sciences, USA 94, 4435–4439.CrossRefGoogle ScholarPubMed
French, PJ, Bunce, CM, Stephens, LR, Lord, JM, McConnell, FM, Brown, G, Creba, JA & Michell, RH (1991) Changes in the levels of inositol lipids and phosphates during the differentiation of HL60 promyelocytic cells towards neutrophils or monocytes. Philosophical Transanctions of the Royal Society of London Series B - Biological Science 245, 193–201.Google ScholarPubMed
Folch, J, Lees, M & Stanley, GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Grases, F, García-Gonzalez, R, Torres, JJ & Llobera, A (1998) The effects of phytic acid on renal stone formation in rats. Scandinavian Journal of Urology and Nephrology 32, 262–265.Google ScholarPubMed
Grases, F, March, JG, Prieto, RM, Simonet, BM, Costa-Bauzá, A, García-Raja, A & Conte, A (2000a) Urinary phytate in calcium oxalate stone-formers and healthy people. Dietary effects on phytate excretion. Scandinavian Journal of Urology and Nephrology 34, 162–164.Google Scholar
Grases, F, Prieto, RM, Simonet, BM & March, JG (2000b) Phytate prevents tissue calcifications in female rats. BioFactors 11, 171–177.CrossRefGoogle ScholarPubMed
Grases, F, Simonet, BM, March, JG & Prieto, RM (2000c) Inositol hexakisphosphate in urine: the relationship between oral intake and urinary excretion. British Journal of Urology International 85, 138–142.CrossRefGoogle ScholarPubMed
Harland, BF & Oberleas, D (1987) Phytate in foods. World Review of Nutrition and Dietetics 52, 235–259.CrossRefGoogle ScholarPubMed
Hawkins, PT, Poyner, DR, Jackson, TR, Letcher, AJ, Lander, DA & Irvine, RF (1993) Inhibition of iron-catalysed hydroxyl radical formation by inositol polyphosphates: a possible physiological function for myo-inositol hexakisphosphate. Biochemical Journal 294, 929–934.CrossRefGoogle ScholarPubMed
Hurrell, RF, Juillerat, MA, Reddy, MB, Lynch, SR, Dassenko, SA & Cook, JD (1992) Soy protein, phytate, and iron absorption in humans. American Journal of Clinical Nutrition 56, 573–578.CrossRefGoogle ScholarPubMed
Irvine, RF (1995) Inositide evolution: What can it tell us about functions? Biochemical Society Transactions 23, 27–35.CrossRefGoogle ScholarPubMed
Jackson, TR, Hallam, TJ, Downes, CP & Hanley, MR (1987) Receptor coupled events in bradykinin action: rapid production of inositol phosphates and regulation of cytosolic free Ca2+ in a neural cell line. EMBO Journal 6, 49–54.CrossRefGoogle Scholar
Kelsay, JL (1987) Effects of fiber, phytic acid and oxalic acid in the diet on mineral bioavailability. American Journal of Gastroenterology 32, 983–986.Google Scholar
Larsson, O, Barker, CJ, Sjöholm Å, A, Carlqvist, H, Michell, RH, Bertorello, A, Nilsson, T, Honkanen, RE, Mayr, GW, Zwiller, J & Berggren, P (1997) Inhibition of phosphatases and increased Ca2+ channel activity by inositol hexakisphosphate. Science 278, 471–474.Google Scholar
Luttrell, BM (1993) The biological relevance of the binding of calcium ions by inositol phosphates. Journal of Biological Chemistry 268, 1521–1524.CrossRefGoogle ScholarPubMed
Maga, JA (1982) Phytate: Its chemistry, occurrence, food interactions, nutritional significance, and methods of analysis. Journal of Agricultural and Food Chemistry 30, 1–9.Google Scholar
March, JG, Simonet, BM, Grases, F & Salvador, A (1998) Indirect determination of phytic acid in urine. Analitica Chimica Acta 367, 63–68.Google Scholar
March, JG, Simonet, BM & Grases, F (2000) Determination of phytic acid by GC–MS. Application to biological samples. Journal of Chromatography B. (In the Press).Google Scholar
March, JG, Villacampa, AI & Grases, F (1995) Enzymatic photometric determination of phytic acid with phytase from aspergillus Ficuum. Analitica Chimica Acta 300, 269–272.Google Scholar
Menniti, FS, Oliver, KG, Putney, JW & Shears, SB (1993) Inositol phosphates and cell signaling: new views of InsP6. Trends in Biological Science 18, 53–56.CrossRefGoogle ScholarPubMed
Ongusaha, PP, Hughes, PJ, Davey, J & Michell, RH (1998) Inositol hexakisphosphate in Schizosaccharomyces pombe: synthesis from Ins (1,4,5)P3 and osmotic regulation. Biochemical Journal 335, 671–679.Google Scholar
O'Rourke, F, Matthews, E & Feinstein, MB (1996) Isolation of InsP4 and InsP6 binding proteins for human platelets: InsP4 promotes Ca2+ efflux from inside-out plasma membrane vesicles containing 104 kDa GAP1IP4BP protein. Biochemical Journal 315, 1027–1034.Google Scholar
Pittet, D, Schlegel, W, Lew, DP, Monod, A & Mayr, GW (1989) Mass changes in inositol tetrakis- and pentakisphosphate isomers induced by chemotactic peptide stimulation in HL-60 cells. Journal of Biological Chemistry 264, 18489–18493.CrossRefGoogle ScholarPubMed
Sandberg, AS, Brune, M, Carlsson, NG, Hallberg, L, Skoglund, E & Rossander-Hulthen, L (1999) Inositol phosphate with different numbers of phosphate groups influence iron absorption in humans. American Journal of Clinical Nutrition 70, 240–246.CrossRefGoogle ScholarPubMed
Sandstrom, B & Sandberg, AS (1992) Inhibitory effects of isolated inositol phosphates on zinc absorption in humans. Journal of Trace Elements and Electrolytes in Health and Disease 6, 99–103.Google Scholar
Sasakawa, N, Sharif, M & Hanley, MR (1995) Metabolism and biological activities of inositol pentakisphosphate and inositol hexakisphosphate. Biochemical Pharmacology 50, 137–146.CrossRefGoogle ScholarPubMed
Stephens, LR & Irvine, RF (1990) Stepwise phosphorylation of myo-inositol leading to myo-inositol hexakisphosphate in Dictyostelium. Nature 346, 580–583.CrossRefGoogle ScholarPubMed
Szwergold, BS, Graham, RA & Brown, TR (1987) Observation of inositol pentakis-hexakis-phosphates in mammalian tissues by 31P NMR. Biochemical and Biophysical Research Communications 149, 874–881.Google Scholar
Vallejo, M, Jackson, T, Lightman, S & Hanley, MR (1987) Occurrence and extracellular actions of inositol pentakis- and hexakisphosphate in mammalian brain. Nature 330, 656–658.CrossRefGoogle ScholarPubMed
York, JD, Odom, AR, Murphy, R, Ives, EB & Wente, SR (1999) A phospholiphase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export. Science 285, 96–100.Google Scholar