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Phospholipid fatty acids of brain and liver are modified by α-tocopherol and dietary fat in growing chicks

Published online by Cambridge University Press:  09 March 2007

H. Fuhrmann
Affiliation:
Institute of Physiological Chemistry, Hannover Veterinary School, Buenteweg 17, D-30559, Hannover, Germany
H. P. Sallmann
Affiliation:
Institute of Physiological Chemistry, Hannover Veterinary School, Buenteweg 17, D-30559, Hannover, Germany
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Abstract

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Dietary fatty acids modify phospholipid fatty acids in brain and liver of growing chickens post-hatching.The effect of vitamin E deficiency on this process is unknown and may be relevant to the pathogenesis of chick nutritional encephalomalacia (NE). Therefore laying hens received a diet low in vitamin E (10 mg α-tocopherol/kg feed). Resulting chicks were assigned to nine dietary groups each fed with either oleic (18:ln-9, 58 g/kg), linoleic (18:2n-6, 57 g/kg) or linolenic (18:3n-3, 56 g/kg) acid together with 5, 25 or 125 mg α-tocopherol/kg feed. NE affecting the cerebellum only occurred in the group given linoleic acid and 5 mg α-tocopherol/kg.In l-d-old chicks and after 1 and 2 weeks the phospholipid fatty acid composition of liver, cerebrum and cerebellum (additionally after 3 weeks) was determined. The feed fatty acids were incorporated into the liver very efficiently during the first week of life. Unsaturation of liver membranes decreased in the orderdietary linolenic >linoleic >oleic acid. In liver, also, the effect of α-tocopherol supplementation on phospholipid fatty acids was most pronounced. Theunsaturation index increased during deficiency, whereas n-9 fatty acids decreased. In the chicken brain the alterations were delayed and less distinct. The cerebellum phospholipids were rich in n-9 fatty acids and as a whole more saturated in comparison with the cerebrum. Cerebellar unsaturation increased when linolenic or linoleic acid was given. However, NE-producing dietary conditions were not accompanied by specific alterations in cerebellar phospholipid fatty acids due to the α-tocopherol content of the diet. Rather the alterations of membrane fatty acids in the liver seem to play a role in the pathogenesis of NE.

Type
Animal Nutrition
Copyright
Copyright © The Nutrition Society 1996

References

REFERENCES

Anderson, G. J. (1994). Developmental sensitivity of the brain to dietary n3 fatty acids. Journal of Lipid Research 35,105111CrossRefGoogle Scholar
Anderson, G. J., Connor, W. E. & Corliss, J. D. (1990). Docosahexaenoic acid is the preferred dietary n-3 fatty acid for the development of the brain and retina. Pediatric Research 27, 8997.CrossRefGoogle ScholarPubMed
Anderson, G. J., Connor, W. E., Corliss, J. D. & Lin, D. S. (1989). Rapid modulation of then-3 docosahexaenoic acid levels in the brain and retina of the newly hatched chick. Journal of Lipid Research 30, 433441.CrossRefGoogle Scholar
Anderson, G. J., van Winkle, S. &Connor, W. E. (1992). Reversibility of the effects of dietary fish oil on the fatty acid composition of the brain and retina of growing chicks. Biochimica er Biophysica Acta 1126, 237246.CrossRefGoogle ScholarPubMed
Aust, S. D., Chignell, C. F., Bray, T. M., Kalyanaraman, B. & Mason, R. P. (1993). Free radicals in toxicology. Toxicology and Applied Pharmacology 120, 168178.CrossRefGoogle ScholarPubMed
Bartov, I. & Bornstein, S. (1980). Susceptibility of chicks to nutritional encephalopathy: effect of fat and α-tocopherol content of the breeder diet. Poultry Science 59, 264267.CrossRefGoogle ScholarPubMed
Bligh, E.G. & Dyer, W. G. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911917.CrossRefGoogle ScholarPubMed
Bourre, J.-M. & Clement, M. (1991). Kinetics of rat peripheral nerve, forebrain and cerebellum α-tocopherol depletion: comparison with different organs. Journal of Nutrition 121, 12041207.CrossRefGoogle ScholarPubMed
Budowski, P., Bartov, I., Dror, Y. & Frankel, E. N. (1979). Lipid oxidation products and chick nutritional encephalopathy. Lipids 14, 768772.CrossRefGoogle ScholarPubMed
Budowski, P. & Crawford, M. A. (1985). α-Linolenic acid as a regulator of the metabolism of arachidonic acid: dietary implications of the ratio, n-6:n-3 fatty acids. Proceedings of the Nutrition Society 44, 221229.CrossRefGoogle Scholar
Budowski, P., Leighfield, M. J. & Crawford, M. A. (1987). Nutritional encephalomalacia in the chick: an exposure of the vulnerable period for cerebellar development and the possible need for both ω6- and ω3-fatty acids. British Journal of Nutrition 58, 511520.CrossRefGoogle Scholar
Buttriss, J. L. & Diplock, A. T. (1988). The relationship between α-tocopherol and phospholipid fatty acids in rat liver subcellular membrane fractions. Biochimica et Biophysica Acta 962, 8190.CrossRefGoogle ScholarPubMed
Chamblee, T. N., Brake, J. D., Schultz, C. D. & Thaxton, J. P. (1992). Yolk sac absorption and initiation of growth in broilers. Poultry Science 71, 18111816.CrossRefGoogle ScholarPubMed
Clement, M. & Bourre, J. M. (1993). Alteration of brain and liver microsomal polyunsaturated fatty acids following vitamin E deficiency. Neuroscience Letters 164, 163166.CrossRefGoogle ScholarPubMed
Cunnane, S. C. (1988). Vitamin E intake affects serum thromboxane and tissue essential fatty acid composition in the rat. Annals of Nutrition and Metabolism 32, 9096.CrossRefGoogle ScholarPubMed
D'Aquino, M., di Felice, M. & Tomassi, G. (1985). Vitamin E status and effects of thennooxidised fats on structural α-tocopherol and fatty acid of different rat tissues. Nutrition Reports International 32, 11791186.Google Scholar
DiMascio, P., Murphy, M. E. & Sies, H. (1991). Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols. American Journal of Clinical Nutrition 53, S194S200.CrossRefGoogle Scholar
Friedman, P. (1986). Dynastar Professional Statistics and Graphics, version 3.3. Washington, DC: Dynamic Microsystems Inc.Google Scholar
Fuhrmann, H., Balthazary, S. T. & Sallmann, H. P. (1994). Bioefficiency of different tocopherols as assessed by haemolysis test and microsomal pentane production. British Journal of Nutrition 71, 605614.CrossRefGoogle ScholarPubMed
Fuhrmann, H. & Sallmann, H. P. (1995). α-Tocopherol and phospholipase A2 in liver and brain of chicks post-hatching: the influence of dietary fat and vitamin E. Annals of Nutrition and Metabolism 39, 302309.CrossRefGoogle Scholar
Garg, M. L., Thomson, A. B. R. & Clandinin, M. T. (1990). Interactions of saturated, n-3 and n-6 polyunsaturated fatty acids to modulate arachidonic acid metabolism. Journal of Lipid Research 31, 271277.CrossRefGoogle ScholarPubMed
Hassan, S., Hakkarainen, J., Jösson, L. & Työppöen, J. (1990). Histopathological and biochemical changes associated with selenium and vitamin E deficiency in chicks. Journal of Veterinary Medicine A 37, 708720.CrossRefGoogle ScholarPubMed
Infante, J. P. (1986). Vitamin E and selenium participation in fatty acid desaturation. A proposal for an enzymatic function of these nutrients. Molecular and Cellular Biochemistry 69, 93108.CrossRefGoogle ScholarPubMed
Jeffcoat, R. & James, A. T. (1984). The regulation of desaturation and elongation of fatty acids in mammals. In Fatty Acid Metabolism and its Regulation, pp. 85112 [Numa, S., editor]. Amsterdam: Elsevier Science Publishers.CrossRefGoogle Scholar
Kaluzny, M. A., Duncan, L. A., Merrit, M. V. & Epps, D. E. (1985). Rapid separation of lipid classes in high yield and purity using bonded phase columns. Journal of Lipid Research 26, 135144.CrossRefGoogle ScholarPubMed
Klaus, A.-M., Fuhrmann, H. & Sallmann, H. P. (1995). Peroxidative and antioxidative metabolism of the broiler chicken as influenced by dietary linoleic acid and vitamin E. Archiv fur Gefluegelkunde 59, 135144.Google Scholar
Leat, W. M. F. (1983). Nutritional deficiencies and fatty acid metabolism. Proceedings of the Nutrition Society 42, 333342.CrossRefGoogle ScholarPubMed
Lepage, G. & Roy, C. C. (1986).Direct transesterification of all classes of lipids in a one-step reaction. Journal of Lipid Research 27, 114120.CrossRefGoogle Scholar
Lin, D. S., Connor, W. E. & Anderson, G. J. (1991). The incorporation of n-3 and n-6 essential fatty acids into the chick embryo from egg yolks having vastly different fatty acid compositions. Pediatric Research 29, 601605.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265275.CrossRefGoogle ScholarPubMed
Moore, S. A., Yoder, E., Murphy, S., Dutton, G. R. & Spector, A. A. (1991). Astrocytes, notneurons, produce docosahexaenoic acid (22: 6ω-3) and arachidonic acid (20:4ω-6). Journal of Neurochemistry 56, 518524.CrossRefGoogle Scholar
Noble, R. C., Cocchi, M. & Bath, H. M. (1993). α-Tocopherol absorption and polyunsaturated fatty acid metabolism in the developing chick embryo. British Poultry Science 34, 815818.CrossRefGoogle ScholarPubMed
Okayasu, T., Kameda, K., Ono, T. & Imai, Y. (1977). Effect of dietary vitamin B2 and vitamin E on the delta-9-desaturase and catalase activities in rat liver microsomes. Biochimica et Biophysica Acta 489, 379402.Google ScholarPubMed
Sallmann, H. P., Fuhrmann, H., Molnar, S. & Stegmanns, T. (1991). Endogenous lipid peroxidation in broiler chickens under dietary loads. Fat Science and Technology 93, 457462.Google Scholar
Sardesai, V. M. (1992). Nutritional role of polyunsaturated fatty acids. Journal of Nutritional Biochemistry 3, 154166.CrossRefGoogle Scholar
Witting, L. A. (1969). Effect of antioxidant-deficiency on phospholipid composition in the chick brain. Journal of Neurochemistry 16, 12531256.CrossRefGoogle ScholarPubMed