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Partition of oleic acid between the lymph and portal blood in rats having a diverted bile-pancreatic duct

Published online by Cambridge University Press:  09 March 2007

Y. Mathieu ,
C. Caselli ,
A. Bernard  and
H. Carlier
Y. Mathieu
Affiliation:
Département de Nutrition, EA DRED 580, Ecole Nationale Supirieure de BiologieAppliquée à la Nutrition et à I 'Alimentalion (ENS.BANA), Université de Bourgogne, 1 Esplanade Erasme, 21000 Dijon, France
C. Caselli
Affiliation:
Département de Nutrition, EA DRED 580, Ecole Nationale Supirieure de BiologieAppliquée à la Nutrition et à I 'Alimentalion (ENS.BANA), Université de Bourgogne, 1 Esplanade Erasme, 21000 Dijon, France
A. Bernard
Affiliation:
Département de Nutrition, EA DRED 580, Ecole Nationale Supirieure de BiologieAppliquée à la Nutrition et à I 'Alimentalion (ENS.BANA), Université de Bourgogne, 1 Esplanade Erasme, 21000 Dijon, France
H. Carlier
Affiliation:
Département de Nutrition, EA DRED 580, Ecole Nationale Supirieure de BiologieAppliquée à la Nutrition et à I 'Alimentalion (ENS.BANA), Université de Bourgogne, 1 Esplanade Erasme, 21000 Dijon, France
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Abstract

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The present study examines the suggestion that in the absence of adequate bile and pancreatic juice, which support the absorption from the gut of long-chain fatty acidsinto lymph, the fatty acids are absorbed directly into the portal blood. Oleic acid (18:l) partitioning between lymph and portal blood was investigated in intact and bile- and pancreatic juice-diverted rats. In a first set of experiments, 18: 1 absorption from the gut into lymph and blood was studied by continuous recovery of the mesenteric lymph for 6 h and mesenteric portal venous blood for 1 h. In a second set of experiments, esterification processes were investigated by study of the mucosal distribution of labelled lipids and by mono- and diacylglycerol acyltransferase (EC 2.3.1.22 and EC 2.3.1.20 respectively) specific activities. In the bile- and pancreatic juice-diverted rats the absorption of labelled 18:l into lymph was significantly reduced during the first 3 h of intraluminal infusion of this substrate. In such rats a compensatory absorption of labelled 18: 1 into mesenteric portal blood was not observed. At 6 h after micellar lipid- mixture infusion, the overload of lipids both in free form and as triacylglycerols persisting in the mucosa paralleled the lower acyltransferase specific activities observed in bile- and pancreatic juice-diverted rats. These studies demonstrate the absence of a previously proposed compensatory absorption of 18: 1 into portal blood when absorption into lymph is impaired by an inadequate supply of bile and pancreatic juice.

Keywords

Chyloportal partitionBileAcylglycerol acyltransferase
Type
Lipid metabolism
Information
British Journal of Nutrition , Volume 75 , Issue 2 , February 1996 , pp. 249 - 261
Copyright
Copyright © The Nutrition Society 1996

References

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